Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   JW980_RS01555 Genome accession   NZ_CP070866
Coordinates   321663..322385 (+) Length   240 a.a.
NCBI ID   WP_004691497.1    Uniprot ID   A0A380U8X3
Organism   Acinetobacter johnsonii strain XY27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 316663..327385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JW980_RS01540 (JW980_01540) ponA 317258..319786 (-) 2529 WP_201701771.1 penicillin-binding protein PBP1a -
  JW980_RS01545 (JW980_01545) pilM 319952..321010 (+) 1059 WP_125273594.1 pilus assembly protein PilM Machinery gene
  JW980_RS01550 (JW980_01550) pilN 321010..321666 (+) 657 WP_005401354.1 PilN domain-containing protein Machinery gene
  JW980_RS01555 (JW980_01555) pilO 321663..322385 (+) 723 WP_004691497.1 type 4a pilus biogenesis protein PilO Machinery gene
  JW980_RS01560 (JW980_01560) pilP 322385..322912 (+) 528 WP_094148569.1 pilus assembly protein PilP Machinery gene
  JW980_RS01565 (JW980_01565) pilQ 322932..325010 (+) 2079 WP_205667517.1 type IV pilus secretin PilQ Machinery gene
  JW980_RS01570 (JW980_01570) - 325099..325581 (+) 483 WP_207778853.1 shikimate kinase -
  JW980_RS01575 (JW980_01575) aroB 325612..326697 (+) 1086 WP_094148567.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 240 a.a.        Molecular weight: 27004.43 Da        Isoelectric Point: 4.9937

>NTDB_id=541134 JW980_RS01555 WP_004691497.1 321663..322385(+) (pilO) [Acinetobacter johnsonii strain XY27]
MSRDEFEELTSENHAVTKKKMTVDKFFQQFNTLDPNNYGSWPFSVKLTCWIFIILVIGALGYFVVIKPKLDAISNAHAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLEQLPKETEIPGLVEDINVSGVNSGLKFKNIRLEPEIRQEFFIEQP
ISIEATGDYHSFGSFVSGIAALPRIVTLHDFDISASSNNEKKTDIPQISYSVKAKTYRYVGATAAASVENTAKADAGGAQ

Nucleotide


Download         Length: 723 bp        

>NTDB_id=541134 JW980_RS01555 WP_004691497.1 321663..322385(+) (pilO) [Acinetobacter johnsonii strain XY27]
ATGAGTCGCGATGAATTTGAAGAATTAACATCAGAAAACCATGCTGTAACCAAGAAGAAAATGACGGTTGATAAGTTTTT
TCAACAGTTTAATACCTTAGATCCAAACAATTACGGTAGTTGGCCCTTTTCAGTCAAATTGACTTGTTGGATCTTTATCA
TTTTAGTGATTGGGGCTTTGGGTTATTTTGTCGTGATTAAGCCGAAACTTGATGCGATTTCAAATGCACATGCACAAGAA
CAAAATCTACTGAATGAGTTTCGCGAAAAAGACTCAAAACTACGTAACTTGCAGCAATATCAAGCGCAATTGCAAGAAAT
GGAAGCGAATTTCAATCAGCAGTTAGAACAGTTGCCGAAAGAAACTGAAATTCCGGGTTTGGTTGAAGACATTAACGTGA
GCGGCGTAAATTCTGGATTAAAGTTTAAAAATATTCGTTTAGAGCCAGAAATTCGCCAAGAGTTCTTTATCGAACAGCCG
ATTTCCATTGAAGCAACGGGTGATTATCATTCCTTTGGTTCATTTGTCAGTGGAATTGCAGCTTTGCCGCGTATTGTGAC
CTTGCATGACTTCGATATCTCAGCATCAAGTAATAATGAGAAAAAGACGGATATTCCGCAAATTTCATACAGTGTAAAGG
CTAAAACTTACCGCTATGTCGGGGCGACAGCTGCTGCAAGTGTGGAAAATACAGCTAAAGCAGATGCAGGAGGTGCTCAG
TAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A380U8X3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

72.385

99.583

0.721
  comO Acinetobacter baylyi ADP1

67.755

100

0.692