Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   I6J48_RS10660 Genome accession   NZ_CP070518
Coordinates   2263908..2264657 (+) Length   249 a.a.
NCBI ID   WP_005038271.1    Uniprot ID   A0AAW8QS31
Organism   Acinetobacter calcoaceticus strain FDAARGOS_1218     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2258908..2269657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J48_RS10645 (I6J48_10645) ponA 2259489..2262050 (-) 2562 WP_205468437.1 penicillin-binding protein PBP1a -
  I6J48_RS10650 (I6J48_10650) pilM 2262212..2263270 (+) 1059 WP_205468438.1 pilus assembly protein PilM Machinery gene
  I6J48_RS10655 (I6J48_10655) pilN 2263270..2263911 (+) 642 WP_205468439.1 PilN domain-containing protein Machinery gene
  I6J48_RS10660 (I6J48_10660) pilO 2263908..2264657 (+) 750 WP_005038271.1 type 4a pilus biogenesis protein PilO Machinery gene
  I6J48_RS10665 (I6J48_10665) pilP 2264668..2265195 (+) 528 WP_003653811.1 pilus assembly protein PilP Machinery gene
  I6J48_RS10670 (I6J48_10670) pilQ 2265258..2267411 (+) 2154 WP_205468440.1 type IV pilus secretin PilQ family protein Machinery gene
  I6J48_RS10675 (I6J48_10675) aroK 2267423..2267992 (+) 570 WP_003653807.1 shikimate kinase AroK -
  I6J48_RS10680 (I6J48_10680) aroB 2268021..2269103 (+) 1083 WP_205468441.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 249 a.a.        Molecular weight: 28118.99 Da        Isoelectric Point: 4.8667

>NTDB_id=540490 I6J48_RS10660 WP_005038271.1 2263908..2264657(+) (pilO) [Acinetobacter calcoaceticus strain FDAARGOS_1218]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITMEATGDYHAFGAFVSSIAALPRIVTMHDFVVEAAPVKDSKSDIPVLNYSIKAKTYRYMGAVENAEHPENNASASVAPV
SSTANTQSK

Nucleotide


Download         Length: 750 bp        

>NTDB_id=540490 I6J48_RS10660 WP_005038271.1 2263908..2264657(+) (pilO) [Acinetobacter calcoaceticus strain FDAARGOS_1218]
ATGAGTCCGGATGAACTTCATGAGTTGTCGCTAGAGCAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTTCT
TCAGCAATTTAACACACTAGATATGAACAGTTATGGTAGCTGGCCATTATCTGTGAAAATTACTTGTTGGATTTTTATTT
TCTTTGCTGTGTTGGCTTTGGGATATTTTGTTGTTATTCAGCCAAAGCTACAAGCAATTGATAATGCACAGGCACAAGAA
AGTAATTTGTTGAATGAGTTTCGAGAAAAAGATTCAAAATTGCGTAACTTGCAGCAATATCAGCTTCAACTTCAAGAGAT
GCAAGCAAACTTTAATCAGCAGTTAGAGCAACTGCCAAAAGAAACCGAAATTCCAAGTTTGGTTGAAGATATTAACTTAA
CTGGGGTGAATTCTGGCCTGAAGTTTAAAAATATCCGTTTAGAAGATGAAGTGAAGCAAGAAATTTTTATTGAACAGCCT
ATTACGATGGAAGCAACCGGCGATTATCACGCTTTTGGTGCTTTTGTCAGTAGTATTGCGGCATTACCACGTATTGTGAC
AATGCATGACTTTGTTGTAGAAGCTGCACCTGTTAAAGATAGTAAGTCAGATATTCCTGTGCTTAATTATTCTATTAAAG
CAAAAACTTATCGCTATATGGGCGCCGTAGAAAATGCTGAACATCCGGAAAACAACGCTTCGGCTTCAGTAGCACCTGTT
TCCTCTACTGCTAATACACAGTCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

83.133

100

0.831
  comO Acinetobacter baylyi ADP1

69.758

99.598

0.695