Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6J48_RS07370 Genome accession   NZ_CP070518
Coordinates   1577604..1579316 (+) Length   570 a.a.
NCBI ID   WP_205469974.1    Uniprot ID   -
Organism   Acinetobacter calcoaceticus strain FDAARGOS_1218     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1572604..1584316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J48_RS07335 (I6J48_07335) nusA 1573490..1574974 (-) 1485 WP_003654584.1 transcription termination factor NusA -
  I6J48_RS07340 (I6J48_07340) rimP 1575011..1575535 (-) 525 WP_005037183.1 ribosome maturation factor RimP -
  I6J48_RS07360 (I6J48_07360) secG 1576176..1576505 (-) 330 WP_005037186.1 preprotein translocase subunit SecG -
  I6J48_RS07365 (I6J48_07365) tpiA 1576518..1577312 (-) 795 WP_003654580.1 triose-phosphate isomerase -
  I6J48_RS07370 (I6J48_07370) pilB 1577604..1579316 (+) 1713 WP_205469974.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6J48_RS07375 (I6J48_07375) pilC 1579343..1580569 (+) 1227 WP_004640845.1 type II secretion system F family protein Machinery gene
  I6J48_RS18485 (I6J48_07380) pilD 1580569..1581429 (+) 861 WP_132071018.1 A24 family peptidase Machinery gene
  I6J48_RS07385 (I6J48_07385) coaE 1581431..1582027 (+) 597 WP_003654573.1 dephospho-CoA kinase -
  I6J48_RS07390 (I6J48_07390) - 1582024..1582938 (-) 915 WP_056483131.1 DMT family transporter -
  I6J48_RS07395 (I6J48_07395) rlmB 1582974..1583723 (-) 750 WP_005037200.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  I6J48_RS07400 (I6J48_07400) - 1583826..1584152 (-) 327 WP_003654567.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63572.94 Da        Isoelectric Point: 5.0251

>NTDB_id=540476 I6J48_RS07370 WP_205469974.1 1577604..1579316(+) (pilB) [Acinetobacter calcoaceticus strain FDAARGOS_1218]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKQDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDINQI
PRDLVDQKLILKHRVLPLVQRGQILYVATSNPNNIEAIDAIRFNSKLMVEQVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLDTSTAPEEDEDTPQGDEAPIVKYVNKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLSFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKVPSDIPKQSLLEMGF
TEQDLTHPDFQVFQPIGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFSNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=540476 I6J48_RS07370 WP_205469974.1 1577604..1579316(+) (pilB) [Acinetobacter calcoaceticus strain FDAARGOS_1218]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAAAGAGCTAAGCAGGATACAGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATATCAACCAGATT
CCTAGAGATTTGGTCGACCAGAAACTGATTTTAAAACATCGGGTCTTGCCTCTCGTACAAAGAGGACAGATTTTATATGT
TGCAACCAGCAATCCCAATAACATTGAAGCTATTGATGCTATTCGCTTCAACAGCAAGCTTATGGTTGAGCAAGTTATTG
TCGAGCACCATAAACTTGAGAAAATTCTGGCGCAGCAATTTACTGAAGAAAGTAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGATCTTGATACCTCAACCGCTCCAGAAGAGGATGAGGATACTCCGCAAGGCGATGAAGCACCTATTGT
TAAATATGTTAACAAGTTATTAATAGATGCGATTCGTATGGGGGCTTCAGACTTGCATTTCGAACCCTATGAGAAATCGT
ATCGGGTCCGTTATCGGGTCGATGGCGTTTTGCGTCAGATTGCAAATCCGCCTTTGCAACTCGCCACTCGGTTAGCTTCA
CGTTTAAAAGTTATGTCTCAAATGGACATTTCCGAAAAGCGTGTTCCTCAAGATGGTCGTATCAAACTCAAGCTATCGAA
ATCTAAAGCAATTGATTTTCGTGTGAACTCTCTTCCGACTTTATTTGGTGAAAAGCTAGTTCTACGTATTCTCGATCCAT
CTAGTGCCATGCTGGGAATTGATGCTCTAGGATATGAAGAAGAGCAAAAAGCACTCTTTATGGAAGCATTAGATAAGCCA
CAAGGTATGCTTTTAATTACTGGTCCTACAGGTTCTGGTAAAACAGTATCCTTATATACGGGCCTCAACATTTTAAACAC
TGAAAGTTCCAATATTTCTACTGCCGAAGATCCAGTCGAAATTAACCTTGAAGGTATTAATCAGGTTAACGTAAACCCTA
AGGTAGGTCTTAGTTTTTCGGCCGCGCTTAAATCATTTTTACGTCAGGATCCTGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACAGCTGAAATCGCGATTAAGGCTGCTCAGACGGGTCACATGGTAATGTCCACACTACATACCAACAGTGC
CCCTGAAACACTTACTCGTCTACGCAATATGGGAGTTCCATCTTTTAATATTGCGACATCGGTCAATTTGGTCATTGCTC
AACGTTTGGCACGTCGATTATGCCCTCAATGTAAGGTGCCTAGTGACATTCCTAAACAAAGCCTATTAGAAATGGGTTTT
ACCGAACAAGATCTAACACATCCCGATTTTCAAGTTTTTCAACCGATCGGTTGTCCTGAATGTCGTGAGGGTTACAAAGG
TCGAGTAGGTATTTATGAAGTAATGAAAGTCACACCTGAGATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAATTGGGGTTTAGTAATCTGCGTCGCTCAGGTTTAAAGAAAGTTATGCAAGGTGTTACTTCT
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

93.158

100

0.932
  pilB Acinetobacter baylyi ADP1

78.772

100

0.788
  pilB Legionella pneumophila strain ERS1305867

51.761

99.649

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.692

93.333

0.482
  pilF Neisseria gonorrhoeae MS11

48.577

98.596

0.479
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384