Detailed information NTG0540468

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I6J48_RS04700
• Genome accession: NZ_CP070518
• Coordinates: 1022606..1023724 (+)
• Length: 372 a.a.
• NCBI ID: WP_004641663.1
• Uniprot ID: A0A0B2UBU9
• Organism: Acinetobacter calcoaceticus strain FDAARGOS_1218
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1017606..1028724

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6J48_RS04690 (I6J48_04690)	-	1020730..1021422 (-)	693	WP_205469757.1	YggS family pyridoxal phosphate-dependent enzyme	-
I6J48_RS04695 (I6J48_04695)	pilT	1021549..1022586 (+)	1038	WP_003653239.1	type IV pilus twitching motility protein PilT	Machinery gene
I6J48_RS04700 (I6J48_04700)	pilU	1022606..1023724 (+)	1119	WP_004641663.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I6J48_RS04705 (I6J48_04705)	fur	1023794..1024231 (-)	438	WP_003653242.1	ferric iron uptake transcriptional regulator	-
I6J48_RS04710 (I6J48_04710)	-	1024343..1024741 (+)	399	WP_003653245.1	outer membrane protein assembly factor BamE	-
I6J48_RS04715 (I6J48_04715)	-	1024809..1025120 (-)	312	WP_205469758.1	RnfH family protein	-
I6J48_RS04720 (I6J48_04720)	-	1025120..1026187 (-)	1068	WP_205469759.1	hypothetical protein	-
I6J48_RS04725 (I6J48_04725)	-	1026339..1026794 (-)	456	WP_017388663.1	bacteriohemerythrin	-
I6J48_RS04730 (I6J48_04730)	-	1027046..1027894 (-)	849	WP_003653249.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41873.07 Da
• Isoelectric Point: 6.6400

>NTDB_id=540468 I6J48_RS04700 WP_004641663.1 1022606..1023724(+) (pilU) [Acinetobacter calcoaceticus strain FDAARGOS_1218]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGDVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNTKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=540468 I6J48_RS04700 WP_004641663.1 1022606..1023724(+) (pilU) [Acinetobacter calcoaceticus strain FDAARGOS_1218]
ATGGATTTTAATGACCTACTCAACCTCATGGTTGAAAAAAAATCATCCGATCTTTTTATTACAGATGGTGTTGCACCATC
TATGAAAATTAACGGGCAAATTGTTCCAATTTCGAAAAATAGTCTTTCTGGGGACGTTATTGGTCAGCTTTTACATTCCA
TCATGAGTGAAAAACAGCGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACGGCC
CGCTTTCGTGTGAGCGCCTTTCAGCAGCGTGACATGCCAGGCATGGTGCTACGCCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCGCCCGTTCTTAAAGATTTATCAATGACCAAACGCGGTATCATTATTTTTGTTGGTGCAACAG
GTACTGGTAAGTCAACGTCTTTGGCTTCAATGATCAGCTATCGCAATCACAACACTAAAGGTCATATCATTACCATTGAA
GACCCAATCGAGTTTATTCATGAGCATGCGGGCTGTATCATTACTCAACGTGAAGTCGGTATTGATACAGACTCATTTGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCAGATGTCATTTTAATTGGTGAGATTCGATCTCGTGAAGTTATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTGGCTACGATGCACGCTAACAACGCCAACCAAGCGCTCGATCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGCCAGCTTTACATGGACTTATCGCTTAACTTAAAGGCCATGATTGCGCA
GCAACTTATTCCAACACCGGATGGCAATTCACGTCGTGCAGCCATTGAGATTTTGATTAACTCGCCATTAATTTCTGACT
ACATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAGCGTTCGCGTGAACTCGGTATGCAAACCTTTGACCAA
GCGTTGTTTGATCTCTATAAAGCAGGCCAAATTACCTATAAAGATGCATTAAAACATGCCGACTCACCGAACGATTTACG
TTTGACCATTAAGCTTGCCGAAGAAGGACCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A0B2UBU9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	67.655	99.731	0.675
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.659	97.849	0.398
pilT	Legionella pneumophila strain Lp02	39.645	90.86	0.36
pilT	Legionella pneumophila strain ERS1305867	39.645	90.86	0.36