Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   LL046_RS12085 Genome accession   NZ_CP086088
Coordinates   2349740..2350741 (-) Length   333 a.a.
NCBI ID   WP_003131994.1    Uniprot ID   Q9CDL3
Organism   Lactococcus lactis subsp. lactis strain EIP13A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2344740..2355741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL046_RS12050 (LL046_12000) - 2345029..2345394 (-) 366 WP_003131985.1 MerR family transcriptional regulator -
  LL046_RS12055 (LL046_12005) - 2345495..2345932 (-) 438 WP_010906378.1 low molecular weight protein-tyrosine-phosphatase -
  LL046_RS12060 (LL046_12010) - 2346076..2346240 (-) 165 WP_003131988.1 hypothetical protein -
  LL046_RS12065 (LL046_12015) - 2346345..2346962 (-) 618 WP_017865237.1 AAA family ATPase -
  LL046_RS12070 (LL046_12020) - 2346959..2348077 (-) 1119 WP_235585843.1 ABC transporter permease -
  LL046_RS12075 (LL046_12025) - 2348209..2348493 (-) 285 WP_226898144.1 hypothetical protein -
  LL046_RS12080 (LL046_12030) - 2348719..2349603 (-) 885 WP_017865240.1 Rgg/GadR/MutR family transcriptional regulator -
  LL046_RS12085 (LL046_12035) ruvB 2349740..2350741 (-) 1002 WP_003131994.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  LL046_RS12090 (LL046_12040) ruvA 2350868..2351461 (-) 594 WP_017865241.1 Holliday junction branch migration protein RuvA Machinery gene
  LL046_RS12095 (LL046_12045) hexB 2351574..2353544 (-) 1971 WP_026138979.1 DNA mismatch repair endonuclease MutL Machinery gene
  LL046_RS12100 (LL046_12050) - 2353673..2354368 (-) 696 WP_017865243.1 hypothetical protein -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 37633.17 Da        Isoelectric Point: 4.5825

>NTDB_id=540328 LL046_RS12085 WP_003131994.1 2349740..2350741(-) (ruvB) [Lactococcus lactis subsp. lactis strain EIP13A]
MNDILNKEPMEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMAFVIANELGVNI
KQTAGPAIEKPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMEDFYIDIMLGSGDGSRSVHLDLPPFTLVGATTR
AGMLSNPLRARFGISSHMEYYQERDLEEIVKRTADIFEVEVIDNAALEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVD
KAITDKALSILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLMRTKQGRKVTQR
AYEHLGYVYNEED

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=540328 LL046_RS12085 WP_003131994.1 2349740..2350741(-) (ruvB) [Lactococcus lactis subsp. lactis strain EIP13A]
ATGAACGATATATTAAACAAAGAGCCAATGGAAGAAGATTACGGTATTGAAAAATCTCTACGTCCGCAATTTTTTAATCA
ATATATTGGTCAAGATAAAGTCAAAGAACAATTAGAAATCTTTATTAAAGCAGCAAAAATGCGTGAAGAAGTTTTGGACC
ACGTGTTACTTTTTGGTCCTCCGGGACTTGGTAAGACAACAATGGCATTTGTCATTGCCAATGAGTTGGGGGTTAATATT
AAACAAACAGCGGGTCCTGCCATTGAAAAGCCAGGAGATTTAGTTGCGATTTTGAATGAATTGGAACCTGGCGATGTGCT
ATTTATTGATGAAATTCATCGGATGCCAATGCAAGTTGAAGAAGTTCTTTATAGCGCTATGGAAGATTTCTACATTGATA
TTATGCTTGGTTCTGGTGATGGTTCACGCTCGGTTCATTTAGATTTGCCGCCATTTACTCTAGTTGGGGCAACAACAAGA
GCTGGAATGCTTTCGAATCCATTGCGTGCCCGTTTTGGAATTTCAAGTCATATGGAGTATTATCAAGAGCGTGATTTAGA
AGAAATTGTTAAAAGAACAGCTGATATTTTTGAAGTCGAAGTTATTGATAATGCCGCTTTAGAGATTGCTTTACGTAGTC
GAGGGACTCCACGGATTGCCAATCGTTTACTTAAACGAGTGCGTGATTTTGCTCAAATAATGGGGGATGGCCGAGTGGAT
AAAGCAATTACCGATAAGGCCTTGTCTATTCTTGATGTTGATGCCGCTGGTTTGGACTATATTGACCAAAAAATTCTCCG
CACAATGATTGAAATGTACCACGGTGGTCCTGTTGGGATTGGAACTTTGGCGGTAAATATTGCCGAAGACCGTGAAACGG
TCGAGGATATGTATGAACCTTATTTGATTCAAAAAGGATTTTTGATGAGAACTAAACAGGGACGTAAGGTGACCCAAAGA
GCCTATGAACACTTAGGTTACGTTTATAATGAAGAAGATTGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-325)

RuvB AAA lid domain (AAA_lid_4)

(181-254)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(20-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CDL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Streptococcus pneumoniae R6

78.614

99.7

0.784
  ruvB Streptococcus pneumoniae D39

78.614

99.7

0.784
  ruvB Streptococcus pneumoniae TIGR4

78.313

99.7

0.781
  ruvB Bacillus subtilis subsp. subtilis str. 168

61.491

96.697

0.595
  ruvB Helicobacter pylori 26695

51.242

96.697

0.495
  ruvB Synechocystis sp. PCC 6803

51.613

93.093

0.48