Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JWV26_RS13375 Genome accession   NZ_CP070505
Coordinates   2901321..2903420 (+) Length   699 a.a.
NCBI ID   WP_196457961.1    Uniprot ID   -
Organism   Ectopseudomonas toyotomiensis strain SM2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2896321..2908420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWV26_RS13355 (JWV26_13355) pilM 2898483..2899547 (+) 1065 WP_017678539.1 pilus assembly protein PilM Machinery gene
  JWV26_RS13360 (JWV26_13360) - 2899547..2900122 (+) 576 WP_206417051.1 PilN domain-containing protein -
  JWV26_RS13365 (JWV26_13365) pilO 2900119..2900745 (+) 627 WP_196457603.1 type 4a pilus biogenesis protein PilO -
  JWV26_RS13370 (JWV26_13370) pilP 2900742..2901266 (+) 525 WP_196457600.1 type 4a pilus biogenesis lipoprotein PilP -
  JWV26_RS13375 (JWV26_13375) pilQ 2901321..2903420 (+) 2100 WP_196457961.1 type IV pilus secretin PilQ Machinery gene
  JWV26_RS13380 (JWV26_13380) aroK 2903426..2903944 (+) 519 WP_206417052.1 shikimate kinase AroK -
  JWV26_RS13385 (JWV26_13385) aroB 2903996..2905099 (+) 1104 WP_072423358.1 3-dehydroquinate synthase -
  JWV26_RS13390 (JWV26_13390) - 2905110..2906759 (+) 1650 WP_206417053.1 AAA family ATPase -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75419.67 Da        Isoelectric Point: 4.7556

>NTDB_id=540328 JWV26_RS13375 WP_196457961.1 2901321..2903420(+) (pilQ) [Ectopseudomonas toyotomiensis strain SM2]
MNNSFSRFGLSVAAFLMSPALLAASLNGLDVAALPGDRVELKLTFDEPIPAPRGYTIEQPARIALDLPGVSNRLGSKNRE
IGMGNARSLTVVEAKDRTRLIVNLTNLAPYTTRVDGNTLFVVVGDGPVSSSPSVSSVPVASPAPVVSAPAARAMAGVRSV
SDIDFQRGEQGEGNIVISLSDPKISPDVQEQGGKIRIDFPKTQLPESLRVRLDVKDFATPVQFVNASSSADRASIVVEPA
GLYDYLVFQADNKLTVSVKPLTEEDAVRRKADRFTYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVRGNITLRLQ
NVPWDQALDLVLKTKGLDQRKVGNVLMVAPADEIAAREQQELAVQKQVAELAPLRKDIIQLNYAKASDIAKLYEEGSSGA
LKDDMRGSVIFDDRTNSLIVTLTQERIDELRRIVTQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNIGSGNWNAWGKN
GNLELGEETDGVQQIDVPFNTPFVDMGVNGSTSGIGIGFITNNTVLDLQLSAMEKSGSGEVVSQPKVVTSDKETAKILKG
TEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRVLMEVKVTKDAPDFSVASSTGGIPAISKNEVNAKVLVADGETIV
IGGVYSNTQSKSVDKVPFLGDLPFIGRVFKRDLVQDRKAELLVFLTPRIMNNQAIAVNR

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=540328 JWV26_RS13375 WP_196457961.1 2901321..2903420(+) (pilQ) [Ectopseudomonas toyotomiensis strain SM2]
ATGAACAACTCGTTCTCGCGATTCGGTCTTTCTGTTGCGGCCTTTTTAATGTCTCCTGCCCTGCTCGCAGCAAGCTTGAA
CGGCTTGGATGTCGCAGCTCTGCCAGGAGACCGGGTCGAGTTGAAATTAACCTTCGATGAGCCTATTCCAGCGCCTCGAG
GCTATACTATCGAGCAGCCTGCACGTATTGCGCTCGACTTGCCGGGTGTATCCAATCGTCTGGGTAGCAAGAATCGTGAA
ATAGGCATGGGCAACGCGCGTAGCCTGACCGTAGTTGAAGCCAAGGATCGCACCCGCCTTATTGTTAATCTGACTAATCT
GGCACCGTATACGACTCGCGTTGACGGCAATACGCTGTTCGTGGTTGTCGGAGATGGGCCAGTATCTTCTTCGCCAAGTG
TCAGCTCTGTGCCGGTTGCAAGCCCTGCTCCTGTCGTATCGGCACCGGCTGCTCGCGCTATGGCTGGTGTTCGTTCTGTG
AGTGACATCGACTTCCAGCGTGGCGAGCAAGGTGAGGGGAATATAGTTATCTCCTTGTCGGATCCCAAGATCAGTCCAGA
TGTTCAAGAGCAGGGAGGGAAGATCCGAATCGACTTTCCCAAGACTCAATTGCCCGAGTCACTGCGTGTGCGGCTAGATG
TAAAGGATTTCGCTACGCCGGTTCAGTTCGTCAATGCCAGTTCCTCCGCTGATCGTGCGAGTATCGTGGTGGAGCCAGCC
GGGCTTTATGATTATCTGGTGTTTCAGGCGGACAACAAGCTGACGGTCAGCGTCAAGCCTCTTACGGAAGAGGATGCCGT
TCGCCGTAAGGCTGATCGCTTCACCTACACAGGTGAAAAGCTTTCTCTGAACTTCCAGGACATTGATGTGCGTTCGGTCC
TGCAGTTGATCGCCGATTTCACGGATCTGAACTTGGTTGCCAGTGATACGGTTCGCGGCAATATCACCTTGCGTCTGCAG
AACGTGCCTTGGGACCAGGCCTTGGATCTGGTGCTCAAGACCAAGGGGCTCGATCAGCGCAAGGTCGGTAACGTTCTGAT
GGTCGCCCCGGCAGATGAGATTGCTGCTCGTGAACAGCAGGAGTTGGCCGTGCAGAAGCAAGTTGCCGAGCTTGCGCCGC
TGCGTAAAGACATCATTCAGTTGAACTACGCGAAGGCATCCGACATTGCCAAACTCTACGAAGAGGGTTCTTCCGGTGCG
CTGAAGGATGATATGCGGGGCTCGGTGATTTTCGATGACCGTACCAATAGTCTCATCGTAACCCTGACTCAGGAGCGTAT
CGACGAATTGCGTCGTATTGTCACCCAGTTGGATATTCCGGTACGCCAAGTAATGATCGAGGCACGTATCGTCGAGGCGA
ATGTCGATTACGACAAGTCGCTTGGTGTTCGCTGGGGTGGGAACATCGGTTCTGGCAATTGGAATGCCTGGGGTAAGAAC
GGTAATCTCGAACTTGGCGAAGAGACCGATGGTGTTCAGCAGATCGATGTTCCTTTCAACACACCTTTCGTTGATATGGG
TGTGAACGGAAGCACCTCGGGTATTGGTATTGGCTTCATTACGAATAACACTGTCTTGGACCTGCAGTTGAGTGCGATGG
AGAAGTCTGGTTCTGGTGAGGTTGTTTCACAGCCGAAGGTCGTGACGTCCGATAAGGAAACCGCGAAGATCCTCAAAGGC
ACCGAAGTGCCTTATCAGGAGGCGAGTTCGAGCGGTGCAACCAGCACTTCTTTCAAAGAGGCTGCGCTATCACTCGAGGT
GACGCCGCAAATCACGCCCGACAATCGCGTTCTCATGGAGGTGAAGGTTACCAAGGATGCTCCTGACTTCTCTGTGGCAT
CTTCAACGGGTGGCATCCCCGCCATCAGCAAGAACGAAGTCAATGCCAAGGTTCTTGTGGCTGATGGTGAGACGATCGTC
ATCGGTGGTGTGTATTCCAACACCCAGTCCAAGTCAGTGGACAAGGTGCCCTTCCTGGGAGATCTCCCTTTTATCGGTCG
GGTGTTCAAGCGTGATCTGGTTCAGGACAGAAAGGCGGAATTGCTTGTATTCCTCACTCCGCGCATCATGAACAACCAAG
CCATTGCCGTGAACCGATGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(304-350)

AMIN domain (AMIN)

(39-122)

Bacterial type II and III secretion system protein (Secretin)

(532-690)

Bacterial type II/III secretion system short domain (Secretin_N)

(378-443)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.983

100

0.742
  pilQ Acinetobacter baumannii strain A118

40.244

100

0.425
  pilQ Acinetobacter baumannii D1279779

40.244

100

0.425
  comQ Acinetobacter baylyi ADP1

40.191

100

0.422