Detailed information NTG0540197

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: JWZ98_RS02245
• Genome accession: NZ_CP070494
• Coordinates: 482799..483956 (+)
• Length: 385 a.a.
• NCBI ID: WP_205454376.1
• Uniprot ID: -
• Organism: Methylomonas sp. EFPC1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 477799..488956

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JWZ98_RS02230 (JWZ98_02230)	-	478828..479250 (-)	423	WP_129871332.1	hypothetical protein	-
JWZ98_RS02235 (JWZ98_02235)	-	479392..480024 (-)	633	WP_033155855.1	response regulator transcription factor	-
JWZ98_RS02240 (JWZ98_02240)	-	480021..482609 (-)	2589	WP_205452960.1	PAS domain S-box protein	-
JWZ98_RS02245 (JWZ98_02245)	pilU	482799..483956 (+)	1158	WP_205454376.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
JWZ98_RS02250 (JWZ98_02250)	-	484187..484510 (-)	324	WP_205452962.1	YqfO family protein	-
JWZ98_RS02255 (JWZ98_02255)	-	484559..484708 (-)	150	WP_165385910.1	hypothetical protein	-
JWZ98_RS02260 (JWZ98_02260)	lexA	484718..485347 (-)	630	WP_205452963.1	transcriptional repressor LexA	-
JWZ98_RS02265 (JWZ98_02265)	-	485744..486004 (-)	261	WP_205452966.1	hypothetical protein	-
JWZ98_RS23100	-	486187..486393 (-)	207	WP_225892983.1	winged helix-turn-helix domain-containing protein	-
JWZ98_RS23105	-	486392..486769 (+)	378	WP_240348182.1	hypothetical protein	-
JWZ98_RS02275 (JWZ98_02275)	-	486919..487638 (-)	720	WP_033155859.1	cytochrome c1	-

Sequence

Protein

• Length: 385 a.a.
• Molecular weight: 42362.60 Da
• Isoelectric Point: 6.3765

>NTDB_id=540197 JWZ98_RS02245 WP_205454376.1 482799..483956(+) (pilU) [Methylomonas sp. EFPC1]
MEFKDYLKILVQHDGSDLYLTAGAPPAAKFQGSLKPLENVKLSRERIKEIADGIMDAEQRAAFEQVPEMNLAITEEGIGR
FRVNIFKQRNCHALVIRNIKVDIPSADALGLPQILKDKIMEKRGLILFVGGTGSGKSTSLAALIDYRNSNSSGHIITIED
PIEFVHPHKKSLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRPQLLMDLSLNLQAFISQRLVPTVEGKRVAAIEILLGTKLVSDLIHKGDIHAIKEAMEKSENIGMQTFDSHL
LKLYKGGVITLEEALRNSDSPNNLKLKINLSEGLGSATKKPSSLAGGLALEEITKKEGDEDSGGH

Nucleotide

• Length: 1158 bp

>NTDB_id=540197 JWZ98_RS02245 WP_205454376.1 482799..483956(+) (pilU) [Methylomonas sp. EFPC1]
ATGGAATTTAAAGACTATCTAAAAATCCTCGTCCAACACGATGGTTCGGACCTTTACCTGACGGCGGGCGCTCCACCGGC
GGCCAAGTTCCAAGGCAGCTTAAAACCGTTGGAAAACGTCAAACTTAGCCGTGAACGCATCAAGGAAATTGCTGACGGTA
TCATGGACGCCGAGCAACGCGCCGCCTTCGAACAGGTGCCGGAAATGAATCTGGCGATCACCGAAGAAGGAATCGGACGG
TTTCGGGTGAATATTTTTAAACAGCGCAATTGCCATGCCTTGGTAATTCGCAACATCAAGGTCGATATTCCCAGTGCCGA
CGCCTTGGGCTTGCCGCAAATCCTCAAAGATAAAATCATGGAAAAGCGCGGGCTGATTTTGTTTGTCGGCGGCACCGGTT
CCGGTAAATCGACGTCCTTGGCGGCCTTGATAGATTATCGCAATAGCAACTCATCCGGGCATATCATTACCATCGAAGAT
CCTATCGAGTTTGTGCATCCGCATAAAAAATCCTTGGTCAATCAACGCGAAGTCGGTGTCGATACCCTCAGTTATGAGGA
TGCATTGAAAAACACTCTGCGGCAGGCGCCGGATGTGATTCTGATTGGCGAAATTCGCAGCCAGGAGACCATGGAACACG
CTTTGGCTTTTGCCGAAACCGGGCATTTGTGCTTGTCCACCTTACACGCCAATAACGCCAATCAGGCCTTGGACCGGATC
ATCAACTTTTTCCCGGAAGAGCGCCGTCCGCAGTTGTTGATGGACTTGTCCTTGAATCTGCAAGCCTTTATTTCCCAGCG
CCTGGTGCCTACTGTCGAAGGTAAGCGGGTGGCGGCTATCGAAATTTTGCTGGGTACTAAATTGGTCAGCGATTTGATCC
ATAAAGGCGATATTCACGCCATCAAAGAAGCGATGGAAAAATCCGAAAATATCGGTATGCAGACTTTTGACAGCCATTTA
CTCAAGCTCTATAAAGGCGGCGTGATCACCTTGGAGGAGGCCTTGCGCAATTCGGATTCGCCGAACAATTTAAAACTTAA
AATCAATTTGAGCGAAGGTTTGGGGTCGGCGACCAAAAAACCCAGTTCTTTGGCCGGCGGTCTTGCTTTGGAAGAAATCA
CCAAGAAAGAGGGTGATGAGGATAGTGGGGGACATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (60-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.959	100	0.561
pilU	Acinetobacter baylyi ADP1	55.367	91.948	0.509
pilU	Vibrio cholerae strain A1552	49.718	91.948	0.457
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.096	94.805	0.39
pilT	Legionella pneumophila strain Lp02	42.727	85.714	0.366
pilT	Legionella pneumophila strain ERS1305867	42.727	85.714	0.366