Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   IUB69_RS12350 Genome accession   NZ_ABACVG020000001
Coordinates   2574792..2576312 (-) Length   506 a.a.
NCBI ID   WP_032185546.1    Uniprot ID   -
Organism   Escherichia coli strain 2020CK-00188     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 2569792..2581312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUB69_RS12340 (IUB69_012305) hdfR 2573470..2574309 (-) 840 WP_032185545.1 HTH-type transcriptional regulator HdfR -
  IUB69_RS12345 (IUB69_012310) maoP 2574428..2574766 (+) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  IUB69_RS12350 (IUB69_012315) comM 2574792..2576312 (-) 1521 WP_032185546.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  IUB69_RS12355 (IUB69_012320) ilvL 2576665..2576763 (+) 99 WP_001311244.1 ilv operon leader peptide -
  IUB69_RS12360 (IUB69_012325) ilvX 2576850..2576900 (+) 51 WP_001387183.1 peptide IlvX -
  IUB69_RS12365 (IUB69_012330) ilvG 2576903..2578549 (+) 1647 WP_001012636.1 acetolactate synthase 2 catalytic subunit -
  IUB69_RS12370 (IUB69_012335) ilvM 2578546..2578809 (+) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  IUB69_RS12375 (IUB69_012340) ilvE 2578829..2579758 (+) 930 WP_000208520.1 branched-chain-amino-acid transaminase -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55231.35 Da        Isoelectric Point: 7.3875

>NTDB_id=53688 IUB69_RS12350 WP_032185546.1 2574792..2576312(-) (comM) [Escherichia coli strain 2020CK-00188]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLTASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=53688 IUB69_RS12350 WP_032185546.1 2574792..2576312(-) (comM) [Escherichia coli strain 2020CK-00188]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCCGGCTTAACTATGGTGGGCTTACCAGAAACAACGGTAAAAGAAGCTCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATCAACCTTGCTCCAGCCGATCTGCCAAAAGAAGGGGGACGA
TATGATTTGCCTATCGCTATTGCGTTACTGACGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGCGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAGGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGCGGACACAACCTTT
TACTGATTGGACCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTGTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGCGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCTCATCACAGTGCGTCGTTAACTGCGATGGTGGGCGGTGGTGCAATTCCAGGGCCTGGTGAAATTTCGCTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGTTACCTGAATTTGAACGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACGCGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTCGCGGCGATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACACCAGAGCAAACGCTGCGTTATCTCAACCGACTCTCTGGTC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCGCCACCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAGCAGCGCCACCGTTAAACAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTG
GCTGGATAGTCCGGAAATACGCCAATTCTGCAAGCTTGAGAGCGAAGATGCGCAGTGGCTGGAAGAAACGCTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTACTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCCGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGTGCCATTGACCGTTTGCTCATCCATCTGCAAAAACTACTGACATA
A

Domains

Predicted by InterProScan.

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.513

100

0.591
  comM Vibrio cholerae O1 biovar El Tor strain E7946

59.163

99.209

0.587
  comM Vibrio cholerae strain A1552

59.163

99.209

0.587
  comM Glaesserella parasuis strain SC1401

58.383

100

0.585
  comM Vibrio campbellii strain DS40M4

57.968

99.209

0.575
  comM Acinetobacter baylyi ADP1

48.996

98.419

0.482
  comM Legionella pneumophila str. Paris

47.992

98.419

0.472
  comM Legionella pneumophila strain ERS1305867

47.992

98.419

0.472
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.071

100

0.441

Multiple sequence alignment