Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   JTE92_RS27770 Genome accession   NZ_CP069812
Coordinates   3325984..3327762 (+) Length   592 a.a.
NCBI ID   WP_063241558.1    Uniprot ID   A0A976GAI0
Organism   Cupriavidus oxalaticus strain Ox1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3320984..3332762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JTE92_RS27735 (JTE92_27735) proB 3321157..3322275 (-) 1119 WP_063241562.1 glutamate 5-kinase -
  JTE92_RS27740 (JTE92_27740) obgE 3322325..3323422 (-) 1098 WP_063241561.1 GTPase ObgE -
  JTE92_RS27745 (JTE92_27745) rpmA 3323546..3323806 (-) 261 WP_010814747.1 50S ribosomal protein L27 -
  JTE92_RS27750 (JTE92_27750) rplU 3323846..3324157 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  JTE92_RS27755 (JTE92_27755) - 3324353..3324646 (-) 294 WP_063241560.1 hypothetical protein -
  JTE92_RS27760 (JTE92_27760) ispB 3324645..3325574 (+) 930 WP_063241559.1 octaprenyl diphosphate synthase -
  JTE92_RS27770 (JTE92_27770) pilF 3325984..3327762 (+) 1779 WP_063241558.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  JTE92_RS27775 (JTE92_27775) - 3327842..3329113 (+) 1272 WP_063241557.1 type II secretion system F family protein -
  JTE92_RS27780 (JTE92_27780) pilD 3329117..3330019 (+) 903 WP_063241556.1 A24 family peptidase Machinery gene
  JTE92_RS27785 (JTE92_27785) coaE 3330071..3330709 (+) 639 WP_063241555.1 dephospho-CoA kinase -
  JTE92_RS27790 (JTE92_27790) zapD 3330947..3331705 (+) 759 WP_063241554.1 cell division protein ZapD -
  JTE92_RS27795 (JTE92_27795) - 3331710..3331901 (+) 192 WP_029048086.1 DNA gyrase inhibitor YacG -
  JTE92_RS27800 (JTE92_27800) - 3331955..3332392 (-) 438 WP_063241553.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 592 a.a.        Molecular weight: 65121.91 Da        Isoelectric Point: 7.7693

>NTDB_id=536874 JTE92_RS27770 WP_063241558.1 3325984..3327762(+) (pilF) [Cupriavidus oxalaticus strain Ox1]
MTLGLALAQSRRIAPALLAQLEQAAREKQSQLIDEIVGSGTMSAHDLALFAADKYQLPLLDLNQYNLTKVPPGLAGNREF
HAHRLLPLGRRENRLVLGLSDPSNQAGLDAIKEKYKLPVEAVVVEHDKLMKYVRSAGEALGTLKNISPVQAERKMIEYDP
VAAAGAQRNRTAADAIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRVRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLIALPKDTKDNKDGKDGKDAKDPKDKDARETVEKAVDFRVSTLPTLFGEKIVMRILESSSDK
LDIDQLGYEPQQKALLLDVIKRPYGMVLVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGL
TFAAALRSFLRQDPDVIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLA
RRLCTCKRPGELPRETLLDAGFREPDLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQQIILSHGTALQIAEQA
RKDGVLSLREAGLLKVRAGVTSLEEVLATTNT

Nucleotide


Download         Length: 1779 bp        

>NTDB_id=536874 JTE92_RS27770 WP_063241558.1 3325984..3327762(+) (pilF) [Cupriavidus oxalaticus strain Ox1]
ATGACACTCGGTCTTGCCCTGGCCCAGAGCCGGCGTATCGCGCCCGCCCTGCTTGCTCAGCTGGAGCAGGCCGCCCGTGA
AAAGCAGTCGCAGCTGATCGATGAGATCGTCGGCAGCGGCACCATGAGCGCGCACGACCTGGCTCTGTTCGCGGCGGACA
AGTACCAGCTGCCGTTGCTGGACCTCAACCAGTACAACCTGACCAAGGTGCCGCCGGGGCTGGCGGGCAACCGCGAATTC
CATGCGCACCGCCTGCTGCCGCTGGGCCGGCGCGAAAACCGGTTGGTGCTGGGGCTGTCCGATCCGTCCAACCAGGCCGG
GCTGGACGCGATCAAGGAAAAGTACAAGCTGCCGGTCGAAGCGGTGGTGGTCGAGCACGACAAGCTGATGAAGTACGTGC
GCTCCGCCGGCGAGGCGCTGGGCACGCTCAAGAACATCTCGCCGGTGCAGGCCGAGCGCAAGATGATCGAATACGACCCG
GTGGCGGCCGCCGGCGCGCAGCGCAACCGCACTGCCGCCGATGCCATCGACGACGCCCCCGTGGTGCGCTTCCTGCAGAA
GCTGCTGACCGAAGCCTTCCACCGCGGCGCATCCGACCTGCATTTCGAGCCGTTCGAGACCTTCTACCGGGTCCGCTTCC
GCGTGGACGGCGTGCTGCAGGAGGTGGCAAGGCCGCCGCTGGATATTCGCGACAAGATCGCCACCCGCATCAAGGTGCTG
TCCCGGCTGGATATTTCTGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCGCGCTGCCCAAGGATACGAA
GGACAACAAGGACGGCAAGGACGGCAAGGACGCGAAGGATCCCAAGGACAAGGACGCCAGGGAAACCGTCGAGAAGGCCG
TCGATTTCCGCGTCTCGACGCTGCCCACGCTGTTCGGCGAGAAGATCGTGATGCGGATCCTGGAATCGTCGTCCGACAAG
CTCGATATCGACCAGCTCGGCTACGAGCCGCAGCAGAAGGCGCTGCTGCTGGACGTGATCAAGCGCCCGTACGGCATGGT
GCTGGTGACCGGCCCCACCGGCAGCGGCAAGACGGTGTCGCTGTACACCTTCCTGAACCTGCTGAACCAGGGCGACATCA
ATATCTCGACCGCCGAGGACCCGGCCGAAATCCAGCTGCCCGGCATCAACCAGGTCAATGTCAACGACAAGGCCGGCCTG
ACCTTTGCCGCGGCGCTGCGCTCGTTCCTGCGGCAGGATCCGGACGTCATCATGGTCGGCGAAATCCGCGACCTGGAGAC
CGCCGATATCTCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCGACGCTGCACACCAACGACGCGCCCACCACGC
TGACGCGGCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCCTCCAGCGTGCTGCTGATCACCGCGCAGCGGCTGGCG
CGGCGGCTGTGCACCTGCAAGCGCCCCGGCGAGCTGCCGCGCGAGACGCTGCTGGATGCGGGCTTCCGCGAACCTGATCT
GGACGGCAGCTGGCAGCCCTACCACCCGGTCGGGTGCGAGCGCTGCAACGGCAGCGGCTACAAGGGCCGCTGCGGCATCT
ACCAGGTCATGCCGATCACCGAGGCGATGCAGCAGATCATCCTGTCGCACGGCACCGCGCTGCAGATCGCCGAGCAGGCG
CGCAAGGACGGCGTGCTATCGTTGCGCGAGGCTGGGCTGCTCAAGGTCAGAGCGGGCGTCACGTCACTCGAAGAGGTGCT
GGCGACCACGAATACGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(288-482)

Type II/IV secretion system protein (T2SSE)

(187-260)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

50.333

100

0.51
  pilB Acinetobacter baumannii D1279779

49.831

100

0.498
  pilB Acinetobacter baylyi ADP1

50

95.946

0.48
  pilB Legionella pneumophila strain ERS1305867

48.506

96.115

0.466
  pilB Vibrio cholerae strain A1552

43.813

100

0.443
  pilB Vibrio campbellii strain DS40M4

45.081

94.426

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

45.063

94.088

0.424
  pilF Thermus thermophilus HB27

37.983

100

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.653

96.453

0.363