Detailed information NTG0535439

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I6J44_RS07450
• Genome accession: NZ_CP069537
• Coordinates: 1548636..1549754 (-)
• Length: 372 a.a.
• NCBI ID: WP_016802720.1
• Uniprot ID: -
• Organism: Acinetobacter pittii strain FDAARGOS_1214
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1543636..1554754

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6J44_RS07420 (I6J44_07420)	-	1544465..1545313 (+)	849	WP_002121748.1	class II glutamine amidotransferase	-
I6J44_RS07425 (I6J44_07425)	-	1545564..1546019 (+)	456	WP_002121698.1	bacteriohemerythrin	-
I6J44_RS07430 (I6J44_07430)	-	1546173..1547240 (+)	1068	WP_005075306.1	hypothetical protein	-
I6J44_RS07435 (I6J44_07435)	-	1547240..1547554 (+)	315	WP_002121714.1	RnfH family protein	-
I6J44_RS07440 (I6J44_07440)	omlA	1547619..1548017 (-)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
I6J44_RS07445 (I6J44_07445)	fur	1548129..1548566 (+)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
I6J44_RS07450 (I6J44_07450)	pilU	1548636..1549754 (-)	1119	WP_016802720.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I6J44_RS07455 (I6J44_07455)	pilT	1549774..1550811 (-)	1038	Protein_1426	type IV pilus twitching motility protein PilT	-
I6J44_RS07460 (I6J44_07460)	yggS	1550938..1551630 (+)	693	WP_016802719.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41959.20 Da
• Isoelectric Point: 6.6404

>NTDB_id=535439 I6J44_RS07450 WP_016802720.1 1548636..1549754(-) (pilU) [Acinetobacter pittii strain FDAARGOS_1214]
MDFNDLLNLMVEKKSSDLFITDGLAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRTREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=535439 I6J44_RS07450 WP_016802720.1 1548636..1549754(-) (pilU) [Acinetobacter pittii strain FDAARGOS_1214]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCCTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAACAGTCTTTCAGGCGAAATTATTGGGCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTGCTACGTCGAATTGAAACCAAAATTCCTTCAAT
TGACGACTTACAATTACCGCCAGTGCTTAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTTGA
AATTGCTTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATCCGTACGCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACGGGTCACTTGGTCTTAGCCACGATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
ACAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATCGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTCTATAAGTCAGGACAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.733	99.731	0.685
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	97.849	0.403
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363