Detailed information NTG0535187

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I6J45_RS12605
• Genome accession: NZ_CP069504
• Coordinates: 2700734..2701852 (+)
• Length: 372 a.a.
• NCBI ID: WP_016802720.1
• Uniprot ID: -
• Organism: Acinetobacter pittii strain FDAARGOS_1215
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2695734..2706852

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6J45_RS12595 (I6J45_12595)	yggS	2698858..2699550 (-)	693	WP_016802719.1	YggS family pyridoxal phosphate-dependent enzyme	-
I6J45_RS12600 (I6J45_12600)	pilT	2699677..2700714 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
I6J45_RS12605 (I6J45_12605)	pilU	2700734..2701852 (+)	1119	WP_016802720.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I6J45_RS12610 (I6J45_12610)	fur	2701922..2702359 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
I6J45_RS12615 (I6J45_12615)	omlA	2702471..2702869 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
I6J45_RS12620 (I6J45_12620)	-	2702934..2703248 (-)	315	WP_002121714.1	RnfH family protein	-
I6J45_RS12625 (I6J45_12625)	-	2703248..2704315 (-)	1068	WP_005075306.1	hypothetical protein	-
I6J45_RS12630 (I6J45_12630)	-	2704469..2704924 (-)	456	WP_002121698.1	bacteriohemerythrin	-
I6J45_RS12635 (I6J45_12635)	-	2705176..2706024 (-)	849	WP_002121748.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41959.20 Da
• Isoelectric Point: 6.6404

>NTDB_id=535187 I6J45_RS12605 WP_016802720.1 2700734..2701852(+) (pilU) [Acinetobacter pittii strain FDAARGOS_1215]
MDFNDLLNLMVEKKSSDLFITDGLAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRTREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=535187 I6J45_RS12605 WP_016802720.1 2700734..2701852(+) (pilU) [Acinetobacter pittii strain FDAARGOS_1215]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCCTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAACAGTCTTTCAGGCGAAATTATTGGGCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTGCTACGTCGAATTGAAACCAAAATTCCTTCAAT
TGACGACTTACAATTACCGCCAGTGCTTAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTTGA
AATTGCTTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATCCGTACGCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACGGGTCACTTGGTCTTAGCCACGATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
ACAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATCGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTCTATAAGTCAGGACAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.733	99.731	0.685
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	97.849	0.403
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363