Detailed information NTG0534620

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: JQN69_RS12420
• Genome accession: NZ_CP069430
• Coordinates: 2572616..2573230 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain TB918
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2567616..2578230

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JQN69_RS12390 (JQN69_12390)	rpsT	2567919..2568185 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
JQN69_RS12395 (JQN69_12395)	holA	2568202..2569243 (-)	1042	Protein_2408	DNA polymerase III subunit delta	-
JQN69_RS12400 (JQN69_12400)	-	2569283..2569434 (-)	152	Protein_2409	hypothetical protein	-
JQN69_RS12405 (JQN69_12405)	-	2569475..2569609 (+)	135	WP_003152870.1	YqzM family protein	-
JQN69_RS12410 (JQN69_12410)	comEC	2569628..2571979 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
JQN69_RS12415 (JQN69_12415)	-	2571980..2572549 (-)	570	WP_094031848.1	ComE operon protein 2	-
JQN69_RS12420 (JQN69_12420)	comEA	2572616..2573230 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
JQN69_RS12425 (JQN69_12425)	comER	2573289..2574110 (+)	822	WP_012118027.1	late competence protein ComER	-
JQN69_RS12430 (JQN69_12430)	-	2574179..2574916 (-)	738	WP_076424998.1	class I SAM-dependent methyltransferase	-
JQN69_RS12435 (JQN69_12435)	rsfS	2574913..2575269 (-)	357	WP_007408260.1	ribosome silencing factor	-
JQN69_RS12440 (JQN69_12440)	yqeK	2575287..2575847 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
JQN69_RS12445 (JQN69_12445)	-	2575837..2576406 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
JQN69_RS12450 (JQN69_12450)	yhbY	2576417..2576707 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
JQN69_RS12455 (JQN69_12455)	aroE	2576701..2577543 (-)	843	WP_094031849.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=534620 JQN69_RS12420 WP_043021637.1 2572616..2573230(-) (comEA) [Bacillus velezensis strain TB918]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=534620 JQN69_RS12420 WP_043021637.1 2572616..2573230(-) (comEA) [Bacillus velezensis strain TB918]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363