Detailed information NTG0534535

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: JQM34_RS06000
• Genome accession: NZ_CP069427
• Coordinates: 1275113..1275574 (-)
• Length: 153 a.a.
• NCBI ID: WP_070568120.1
• Uniprot ID: A0A1E9G859
• Organism: Streptococcus oralis strain SF100
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1270113..1280574

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JQM34_RS05970 (JQM34_0001217)	-	1270523..1271350 (-)	828	WP_000728097.1	membrane protein insertase YidC	-
JQM34_RS05975 (JQM34_0001218)	rnpA	1271325..1271696 (-)	372	WP_000748120.1	ribonuclease P protein component	-
JQM34_RS05980 (JQM34_0001219)	-	1271846..1273036 (-)	1191	WP_049490206.1	acetate kinase	-
JQM34_RS05985 (JQM34_0001220)	comYH	1273087..1274040 (-)	954	WP_049490205.1	class I SAM-dependent methyltransferase	Machinery gene
JQM34_RS05990 (JQM34_0001221)	-	1274099..1274689 (-)	591	WP_049490204.1	class I SAM-dependent methyltransferase	-
JQM34_RS05995 (JQM34_0001222)	comGG/cglG	1274722..1275135 (-)	414	WP_070568123.1	competence type IV pilus minor pilin ComGG	Machinery gene
JQM34_RS06000 (JQM34_0001223)	comGF/cglF	1275113..1275574 (-)	462	WP_070568120.1	competence type IV pilus minor pilin ComGF	Machinery gene
JQM34_RS06005 (JQM34_0001224)	comGE/cglE	1275537..1275839 (-)	303	WP_200370534.1	competence type IV pilus minor pilin ComGE	Machinery gene
JQM34_RS06010 (JQM34_0001225)	comGD/cglD	1275802..1276236 (-)	435	WP_200370535.1	competence type IV pilus minor pilin ComGD	Machinery gene
JQM34_RS06015 (JQM34_0001226)	comGC/cglC	1276199..1276522 (-)	324	WP_200370536.1	competence type IV pilus major pilin ComGC	Machinery gene
JQM34_RS06020 (JQM34_0001227)	comGB/cglB	1276524..1277540 (-)	1017	WP_000355379.1	competence type IV pilus assembly protein ComGB	Machinery gene
JQM34_RS06025	comGA	1277488..1278428 (-)	941	Protein_1185	competence type IV pilus ATPase ComGA	-
JQM34_RS06030 (JQM34_0001230)	-	1278498..1278863 (-)	366	WP_000286396.1	DUF1033 family protein	-
JQM34_RS06035 (JQM34_0001231)	nagA	1279018..1280169 (-)	1152	WP_001134447.1	N-acetylglucosamine-6-phosphate deacetylase	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17914.66 Da
• Isoelectric Point: 9.6234

>NTDB_id=534535 JQM34_RS06000 WP_070568120.1 1275113..1275574(-) (comGF/cglF) [Streptococcus oralis strain SF100]
MVQRSCWILKSSKVRAFTLLESLIALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEVELERSQFEKVEGN
RLYMKQDGKDISIGKSKSDDFRKTDTSGRGYQPMVYGLKSAQITEENQLVRFRFQFQKGLEREFIYRVEKAKS

Nucleotide

• Length: 462 bp

>NTDB_id=534535 JQM34_RS06000 WP_070568120.1 1275113..1275574(-) (comGF/cglF) [Streptococcus oralis strain SF100]
ATGGTTCAGAGAAGTTGCTGGATCTTAAAGAGCAGTAAGGTAAGAGCGTTCACTCTTTTAGAATCTCTGATTGCTCTTAT
CGTCATTAGCGGAGGCTTGCTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGTTACCAGCAGCAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGATCAGCTGGAGGTAGAGTTAGAGCGTTCGCAGTTTGAAAAGGTAGAAGGCAAT
CGCCTCTACATGAAACAGGATGGTAAGGATATCTCTATAGGGAAGTCTAAATCGGATGATTTTCGAAAAACGGATACCAG
CGGACGGGGGTATCAGCCGATGGTTTATGGACTCAAATCAGCACAGATTACAGAGGAAAACCAATTGGTTCGCTTTCGCT
TCCAATTTCAAAAGGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGCAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

3D structure

• AlphaFold DB: A0A1E9G859

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus mitis SK321	91.503	100	0.915
comGF/cglF	Streptococcus mitis NCTC 12261	90.196	100	0.902
comGF/cglF	Streptococcus pneumoniae Rx1	89.542	100	0.895
comGF/cglF	Streptococcus pneumoniae D39	89.542	100	0.895
comGF/cglF	Streptococcus pneumoniae R6	89.542	100	0.895
comGF/cglF	Streptococcus pneumoniae TIGR4	89.542	100	0.895
comGF	Lactococcus lactis subsp. cremoris KW2	40.714	91.503	0.373