Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JN757_RS23940 Genome accession   NZ_CP069352
Coordinates   5479068..5480768 (+) Length   566 a.a.
NCBI ID   WP_203419529.1    Uniprot ID   -
Organism   Pseudomonas granadensis isolate CT364     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5474068..5485768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN757_RS23920 (JN757_23910) - 5474254..5477205 (+) 2952 WP_203419526.1 DUF748 domain-containing protein -
  JN757_RS23925 (JN757_23915) - 5477205..5477501 (+) 297 WP_093098244.1 DUF2845 domain-containing protein -
  JN757_RS23930 (JN757_23920) - 5477769..5478122 (-) 354 WP_203419527.1 BON domain-containing protein -
  JN757_RS23935 (JN757_23925) - 5478420..5478842 (-) 423 WP_203419528.1 pilin -
  JN757_RS23940 (JN757_23930) pilB 5479068..5480768 (+) 1701 WP_203419529.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JN757_RS23945 (JN757_23935) pilC 5480771..5481988 (+) 1218 WP_203419530.1 type II secretion system F family protein Machinery gene
  JN757_RS23950 (JN757_23940) pilD 5481990..5482862 (+) 873 WP_203419531.1 A24 family peptidase Machinery gene
  JN757_RS23955 (JN757_23945) coaE 5482859..5483482 (+) 624 WP_203419532.1 dephospho-CoA kinase -
  JN757_RS23960 (JN757_23950) yacG 5483479..5483679 (+) 201 WP_093098232.1 DNA gyrase inhibitor YacG -
  JN757_RS23965 (JN757_23955) - 5483686..5483901 (-) 216 WP_203419533.1 hypothetical protein -
  JN757_RS23970 (JN757_23960) - 5483967..5484656 (-) 690 WP_203419534.1 energy-coupling factor ABC transporter permease -
  JN757_RS23975 (JN757_23965) - 5484774..5485400 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62703.72 Da        Isoelectric Point: 5.8308

>NTDB_id=534140 JN757_RS23940 WP_203419529.1 5479068..5480768(+) (pilB) [Pseudomonas granadensis isolate CT364]
MNDIALSGLAKQLVQAELLTEQSAQQAWQQAQRNRVSLVNYLVHNKLVKSRQIAEIASEHFGMALLDLSCLDKETQPKGL
VSEKLIRQHCALPLWRRGNKLFVGLSDPSNQQAISDIQFSTGLSTEAILVEDDKLSDAIDKFFDSHATGLEEMADVDLDG
LDVESVSDSKQDTIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGMLREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKELYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCNHCKKPIEVPRETLIKEGFPE
ERIGSFTIYEPVGCDHCNSGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDMQMRRDGFDDLRTAGLHKAMQGTTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=534140 JN757_RS23940 WP_203419529.1 5479068..5480768(+) (pilB) [Pseudomonas granadensis isolate CT364]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTGACGGAACAAAGTGCCCAGCAAGC
CTGGCAACAGGCGCAGCGCAATCGCGTGTCGCTGGTGAACTACCTGGTGCACAACAAACTGGTGAAGAGCCGGCAGATCG
CCGAGATTGCCTCGGAACACTTTGGCATGGCCCTGCTCGACCTCAGTTGCCTCGATAAGGAAACCCAGCCAAAGGGCCTG
GTCAGCGAAAAACTGATACGCCAGCACTGCGCTCTACCATTGTGGCGACGCGGCAACAAACTGTTCGTGGGCCTCTCCGA
CCCGAGCAACCAGCAGGCAATCAGTGATATTCAGTTCAGTACAGGCCTGAGTACCGAAGCGATTCTGGTCGAGGATGACA
AGCTCAGCGATGCCATCGACAAGTTCTTCGACAGTCATGCCACCGGCCTGGAAGAAATGGCCGACGTCGACCTCGATGGC
CTTGACGTCGAATCCGTCAGCGACAGCAAACAGGACACCATCGGCGGGCTCGATGCCGACGATGCGCCGGTGGTGCGCTT
CGTTCACAAGATGCTGCTCGACGCGATCAAAAGCGGCTCCTCCGACCTGCACTTCGAACCTTATGAGAAGAACTATCGGG
TGCGGGTGCGCACCGACGGCATGCTGCGTGAAGTGGCCAAACCGCCGATTCAACTGGCCGGGCGCATCGCCGCGCGCCTG
AAGGTCATGGCCAGCCTCGATATCTCGGAGCGACGCAAACCGCAGGACGGGCGGATCAAGATGCGCCTGTCGAAAAGCAA
GTCGATCGACTTCCGGGTCAATACCCTGCCGACGCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGCG
CGCAGATGGGTATCGATGCGCTCGGTTATGAACCGGAACAGAAAGAGTTGTACATGGCCGCGCTGAAACAGCCGCAAGGC
ATGATTCTGGTGACCGGCCCTACCGGGTCGGGGAAAACCGTGTCGCTGTACACCGGGCTCAATATCCTCAACACCGTCGA
TATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAATCAGGTCAACGTCAATCCGCGCCAAG
GGCTGGACTTTGCCCAGGCCCTGCGTTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATTGCCATCAAAGCCGCGCAGACCGGTCACCTCGTGCTCTCGACCCTGCACACCAACAGCGCCGCAGA
AACCCTCACCCGTCTGCACAACATGGGCATTCCCGGTTTCAACATTGCTACCTCGGTCAGTCTGATCATCGCTCAGCGCC
TGGCACGCAAGCTGTGCAATCACTGCAAGAAACCTATTGAAGTGCCACGTGAAACCCTGATCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCAGTTTCACGATCTACGAGCCGGTCGGTTGCGATCATTGCAACAGCGGTTACAAGGGACGCGTGGGGAT
TTATGAAGTGGTGAAGAACACACCTGAGCTGCAACGCCTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATATGCAGA
TGCGCCGCGACGGCTTCGACGATCTGCGCACCGCAGGTCTGCACAAGGCCATGCAAGGCACTACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.761

99.823

0.587
  pilB Acinetobacter baumannii D1279779

57.27

99.647

0.571
  pilB Legionella pneumophila strain ERS1305867

56.537

100

0.565
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

50.267

99.117

0.498
  pilF Neisseria gonorrhoeae MS11

49.645

99.647

0.495
  pilB Vibrio campbellii strain DS40M4

48.582

99.647

0.484