Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JN757_RS03050 Genome accession   NZ_CP069352
Coordinates   686903..688981 (+) Length   692 a.a.
NCBI ID   WP_203420410.1    Uniprot ID   -
Organism   Pseudomonas granadensis isolate CT364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 681903..693981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN757_RS03030 (JN757_03030) comM 684115..685179 (+) 1065 WP_090280418.1 pilus assembly protein PilM Machinery gene
  JN757_RS03035 (JN757_03035) - 685179..685745 (+) 567 WP_203420407.1 PilN domain-containing protein -
  JN757_RS03040 (JN757_03040) pilO 685742..686365 (+) 624 WP_203420408.1 type 4a pilus biogenesis protein PilO -
  JN757_RS03045 (JN757_03045) - 686362..686889 (+) 528 WP_203420409.1 pilus assembly protein PilP -
  JN757_RS03050 (JN757_03050) pilQ 686903..688981 (+) 2079 WP_203420410.1 type IV pilus secretin PilQ Machinery gene
  JN757_RS03055 (JN757_03055) aroK 688986..689504 (+) 519 WP_090280433.1 shikimate kinase AroK -
  JN757_RS03060 (JN757_03060) aroB 689657..690757 (+) 1101 WP_203420411.1 3-dehydroquinate synthase -
  JN757_RS03065 (JN757_03065) - 690768..692360 (+) 1593 WP_203420412.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75082.44 Da        Isoelectric Point: 5.4130

>NTDB_id=534127 JN757_RS03050 WP_203420410.1 686903..688981(+) (pilQ) [Pseudomonas granadensis isolate CT364]
MNRIFSTLGFSLWIALMSPMVNAANLKALDVAALPGDRVELKLSFDSPPPAPKGYTTESPARIAVDLPGVANQLSNKSLD
LGSGNARTATVVEAKDRTRLIVSLTQLAPYTTRIDGNTLFVVVGQGAPAPASRPAAVAQRVSTAAAPAAKPFVPKNRGIR
GVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAAVSGDRTVITVEPSG
TYDYSTFQTDNKLTVSIRPMSVDDLQKRNAERQAYIGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GSSSTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=534127 JN757_RS03050 WP_203420410.1 686903..688981(+) (pilQ) [Pseudomonas granadensis isolate CT364]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTAAACGCGGCCAACCTGAA
AGCGCTGGATGTCGCGGCGTTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGACAGTCCGCCGCCGGCGCCCAAGG
GCTATACCACCGAATCGCCGGCGCGCATCGCGGTGGATCTGCCCGGTGTCGCCAATCAATTGAGCAACAAGAGTCTGGAT
CTGGGCAGCGGCAATGCGCGTACCGCCACGGTGGTCGAGGCCAAGGACCGCACGCGGCTGATCGTCAGCCTCACCCAGCT
TGCGCCTTACACCACGCGCATCGATGGCAATACTCTGTTTGTGGTGGTTGGCCAGGGTGCACCGGCGCCTGCATCGCGCC
CCGCCGCGGTTGCGCAGCGTGTGAGCACGGCAGCTGCGCCAGCGGCCAAGCCATTCGTACCAAAAAACCGCGGCATTCGT
GGCGTCGATTTCCAGCGCGGCACCGAGGGCGAGGGCAACGTTGTCATTGATCTGTCCGACCCGACCATTGCCCCGGATAT
CCAGGAACACGACGGCAAAATCATCCTCAGCTTCGCCCGCACACAGCTGCCGGAAAAGCTGCGCGTGCGCCTCGACGTCA
AGGATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGCGGTGAGTGGTGATCGCACGGTGATCACCGTCGAGCCCAGCGGC
ACCTACGACTATTCGACCTTCCAGACCGACAACAAACTGACCGTCAGCATCCGCCCAATGAGCGTCGACGACCTGCAAAA
GCGCAACGCCGAGCGTCAGGCCTACATTGGCGAAAAGCTCTCGCTGAATTTTCAGGACATCGATGTGCGCTCGGTGCTGC
AACTGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGCATCACCTTGCGCCTGCAAAAC
GTACCGTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGGCTGGATAAACGCAAGATCGGCAACGTGCTGCTGGT
CGCGCCGGCCGATGAGATCGCTGCCCGCGAACGCCAGGAACTCGAGTCGCAGAAGCAGATTGCCGAACTGGCGCCGCTGC
GTCGTGAACTATTGCAGGTGAATTACGCCAAGGCGGCGGACATTGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAAGCG
AAAATCGATGAGCGCGGTTCGATCACCGTGGATGAGCGGACCAACAATATCATTGCCTACCAGACCCAGGATCGTCTCGA
CGAGCTGCGGCGAATCGTGGCGCAACTGGATATACCGGTGCGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAACG
TCGATTACGACAAGAGTCTGGGCGTGCGCTGGGGCGGTTCAGTGCAGAACAAGGGCAACTGGAACGCGTCCGGGGTCAAC
GGTTCATCTTCCACCATCGGCACGCCGGGGAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGTACCGTCAACAATAC
TTCCGGGATTGGCATCGCCTTCATTACCGATAACGTGCTGCTCGACCTTGAACTGACGGCGATGGAGAAGACCGGCAACG
GCGAAATCGTCTCGCAACCGAAAGTGGTCACCTCCGACAAAGAAACCGCGAAGATCCTCAAAGGCACGGAGATTCCCTAT
CAGGAAGCCAGCTCCAGTGGCGCGACTTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAGGTCACGCCGCAGATTAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGATTACCTGAACAAAGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGGTCAATGACGGCGAAACCATCGTGATTGGCGGTGTTTTTTCAAAT
ACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.486

100

0.75
  pilQ Acinetobacter baumannii strain A118

38.172

100

0.41
  pilQ Acinetobacter baumannii D1279779

38.172

100

0.41
  comQ Acinetobacter baylyi ADP1

39.884

100

0.399
  pilQ Legionella pneumophila strain Lp02

37.83

98.555

0.373
  pilQ Legionella pneumophila strain ERS1305867

37.703

98.121

0.37