Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JNO62_RS14645 Genome accession   NZ_CP069245
Coordinates   3158244..3158765 (-) Length   173 a.a.
NCBI ID   WP_011000516.1    Uniprot ID   Q8Y1Y0
Organism   Ralstonia pseudosolanacearum strain PeaFJ1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3153244..3163765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNO62_RS14630 (JNO62_14540) moaC 3154938..3155414 (+) 477 WP_011000519.1 cyclic pyranopterin monophosphate synthase MoaC -
  JNO62_RS14635 (JNO62_14545) - 3155642..3157375 (-) 1734 WP_111374452.1 PglL family O-oligosaccharyltransferase -
  JNO62_RS14640 (JNO62_14550) pilA 3157468..3157977 (-) 510 WP_111374451.1 pilin Machinery gene
  JNO62_RS14645 (JNO62_14555) pilA 3158244..3158765 (-) 522 WP_011000516.1 pilin Machinery gene
  JNO62_RS14650 (JNO62_14560) - 3158959..3159657 (-) 699 WP_019717569.1 TerC family protein -
  JNO62_RS14655 (JNO62_14565) sucD 3159807..3160688 (-) 882 WP_011000514.1 succinate--CoA ligase subunit alpha -
  JNO62_RS14660 (JNO62_14570) sucC 3160773..3161939 (-) 1167 WP_011000513.1 ADP-forming succinate--CoA ligase subunit beta -
  JNO62_RS14665 (JNO62_14575) - 3162006..3162632 (-) 627 WP_011000512.1 DUF2889 domain-containing protein -
  JNO62_RS14670 (JNO62_14580) recX 3162907..3163386 (-) 480 WP_111374450.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17844.54 Da        Isoelectric Point: 10.0311

>NTDB_id=533152 JNO62_RS14645 WP_011000516.1 3158244..3158765(-) (pilA) [Ralstonia pseudosolanacearum strain PeaFJ1]
MASKHSRNRRRGQRGFTLIELMIVVAIIGILAALAIPAYQDYTIRSRVVEGLALAGQAKALVVENAANAQSSLSLGSASL
PASKNVSALNIDSSTGEIAVVYAPAVTEAGANTLVLTPYSGSSSVFGNLQAGSTPPAMVLWVCAAAGKSMPVNTVTQRTT
ATLPPKYAPSECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=533152 JNO62_RS14645 WP_011000516.1 3158244..3158765(-) (pilA) [Ralstonia pseudosolanacearum strain PeaFJ1]
ATGGCATCGAAGCACAGCAGGAACAGGCGGCGTGGCCAGCGCGGCTTTACATTGATCGAGTTGATGATCGTGGTGGCGAT
CATCGGGATTTTGGCGGCGCTGGCGATTCCGGCGTATCAGGACTACACGATCCGGTCGCGTGTGGTCGAGGGGCTGGCAC
TGGCAGGGCAAGCCAAGGCGCTGGTGGTTGAAAATGCCGCCAACGCACAGAGTAGCCTGTCACTCGGTTCCGCATCGTTG
CCGGCCAGCAAGAACGTCAGTGCGCTGAATATTGATTCGTCCACCGGCGAAATCGCAGTGGTCTATGCACCCGCTGTTAC
GGAAGCGGGGGCGAATACGTTGGTATTGACCCCATACTCCGGCAGTTCGTCAGTATTTGGCAATTTGCAGGCAGGTTCAA
CGCCGCCGGCAATGGTGCTGTGGGTGTGTGCCGCCGCCGGCAAGTCGATGCCGGTCAATACCGTTACCCAGAGGACGACG
GCTACGTTGCCACCGAAATACGCGCCCTCGGAGTGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(45-173)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8Y1Y0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

51.705

100

0.526
  pilA2 Legionella pneumophila str. Paris

44.848

95.376

0.428
  pilA2 Legionella pneumophila strain ERS1305867

44.242

95.376

0.422
  comP Acinetobacter baylyi ADP1

39.773

100

0.405
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.938

100

0.399