Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JNO62_RS14640 Genome accession   NZ_CP069245
Coordinates   3157468..3157977 (-) Length   169 a.a.
NCBI ID   WP_111374451.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain PeaFJ1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3152468..3162977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNO62_RS14620 (JNO62_14530) - 3152554..3153141 (+) 588 WP_111374454.1 DUF2946 family protein -
  JNO62_RS14625 (JNO62_14535) - 3153156..3154844 (-) 1689 WP_111374453.1 tetratricopeptide repeat protein -
  JNO62_RS14630 (JNO62_14540) moaC 3154938..3155414 (+) 477 WP_011000519.1 cyclic pyranopterin monophosphate synthase MoaC -
  JNO62_RS14635 (JNO62_14545) - 3155642..3157375 (-) 1734 WP_111374452.1 PglL family O-oligosaccharyltransferase -
  JNO62_RS14640 (JNO62_14550) pilA 3157468..3157977 (-) 510 WP_111374451.1 pilin Machinery gene
  JNO62_RS14645 (JNO62_14555) pilA 3158244..3158765 (-) 522 WP_011000516.1 pilin Machinery gene
  JNO62_RS14650 (JNO62_14560) - 3158959..3159657 (-) 699 WP_019717569.1 TerC family protein -
  JNO62_RS14655 (JNO62_14565) sucD 3159807..3160688 (-) 882 WP_011000514.1 succinate--CoA ligase subunit alpha -
  JNO62_RS14660 (JNO62_14570) sucC 3160773..3161939 (-) 1167 WP_011000513.1 ADP-forming succinate--CoA ligase subunit beta -
  JNO62_RS14665 (JNO62_14575) - 3162006..3162632 (-) 627 WP_011000512.1 DUF2889 domain-containing protein -

Sequence

Protein


Download         Length: 169 a.a.        Molecular weight: 17251.99 Da        Isoelectric Point: 9.9491

>NTDB_id=533151 JNO62_RS14640 WP_111374451.1 3157468..3157977(-) (pilA) [Ralstonia pseudosolanacearum strain PeaFJ1]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTVRARVTEGLSLAAQAKALVSENAANAQSDLSVGSSVFT
ATKNVANLQILGTDATAPGQITVTYTTAAGGGTLALVPTAAGAKLPLSSAPSGPIMWTCYAQNKATAASSVPPSGTMTLA
AKYAPAECR

Nucleotide


Download         Length: 510 bp        

>NTDB_id=533151 JNO62_RS14640 WP_111374451.1 3157468..3157977(-) (pilA) [Ralstonia pseudosolanacearum strain PeaFJ1]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACGGTTCGTGCACGCGTGACGGAAGGTCTGTCGCTGG
CTGCACAAGCCAAGGCACTGGTGTCGGAAAACGCTGCCAATGCACAGTCTGACCTGTCGGTGGGTTCGTCGGTGTTCACG
GCCACCAAGAACGTTGCCAACCTCCAGATCCTCGGTACGGATGCTACTGCTCCGGGCCAGATCACCGTTACCTATACGAC
GGCAGCTGGCGGCGGCACGCTGGCTCTGGTTCCGACTGCGGCAGGCGCTAAGCTGCCGTTGAGCTCCGCACCGTCGGGCC
CGATCATGTGGACCTGCTACGCTCAAAACAAGGCCACTGCTGCCAGCAGCGTTCCTCCGAGTGGTACGATGACGCTGGCG
GCCAAGTATGCCCCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)

Pilin (bacterial filament) (Pilin)

(43-169)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

92.899

100

0.929
  pilA2 Legionella pneumophila str. Paris

50.617

95.858

0.485
  pilA2 Legionella pneumophila strain ERS1305867

50

95.858

0.479
  comP Acinetobacter baylyi ADP1

43.787

100

0.438
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.979

100

0.42