Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   LLI11_RS11675 Genome accession   NZ_CP069223
Coordinates   2265543..2266616 (-) Length   357 a.a.
NCBI ID   WP_014570824.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain I.1.1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2227450..2265392 2265543..2266616 flank 151


Gene organization within MGE regions


Location: 2227450..2266616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLI11_RS11390 (LLI11_11330) - 2227450..2227887 (-) 438 WP_003129992.1 zinc-dependent MarR family transcriptional regulator -
  LLI11_RS11395 (LLI11_11335) - 2228094..2229041 (+) 948 WP_003130410.1 IS30 family transposase -
  LLI11_RS11400 (LLI11_11340) comGG 2229015..2229341 (-) 327 WP_058221568.1 competence type IV pilus minor pilin ComGG Machinery gene
  LLI11_RS11405 (LLI11_11345) comGF 2229391..2229819 (-) 429 Protein_2221 competence type IV pilus minor pilin ComGF -
  LLI11_RS11410 (LLI11_11350) comGE 2229782..2230078 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLI11_RS11415 (LLI11_11355) comGD 2230050..2230481 (-) 432 WP_080585155.1 competence type IV pilus minor pilin ComGD Machinery gene
  LLI11_RS11420 (LLI11_11360) comGC 2230441..2230710 (-) 270 WP_003129998.1 competence type IV pilus major pilin ComGC Machinery gene
  LLI11_RS11425 (LLI11_11365) - 2230837..2231529 (-) 693 WP_152994386.1 hypothetical protein -
  LLI11_RS11430 (LLI11_11370) - 2231771..2232550 (-) 780 WP_082225220.1 peptidoglycan amidohydrolase family protein -
  LLI11_RS11435 (LLI11_11375) - 2232550..2232849 (-) 300 WP_031559226.1 phage holin -
  LLI11_RS11440 (LLI11_11380) - 2232862..2233212 (-) 351 WP_014570798.1 hypothetical protein -
  LLI11_RS11445 (LLI11_11385) - 2233225..2233461 (-) 237 WP_082225257.1 hypothetical protein -
  LLI11_RS11450 (LLI11_11390) - 2233473..2237738 (-) 4266 WP_243525488.1 hypothetical protein -
  LLI11_RS11455 (LLI11_11395) - 2237717..2239246 (-) 1530 WP_003131327.1 distal tail protein Dit -
  LLI11_RS11460 (LLI11_11400) - 2239256..2241865 (-) 2610 WP_058221395.1 phage tail tape measure protein -
  LLI11_RS11465 (LLI11_11405) - 2241855..2242562 (-) 708 WP_003131324.1 Gp15 family bacteriophage protein -
  LLI11_RS11470 (LLI11_11410) - 2242578..2242985 (-) 408 WP_058221396.1 hypothetical protein -
  LLI11_RS11475 (LLI11_11415) - 2243042..2243518 (-) 477 WP_014570559.1 phage tail tube protein -
  LLI11_RS11480 (LLI11_11420) - 2243529..2243963 (-) 435 WP_003131321.1 minor capsid protein -
  LLI11_RS11485 (LLI11_11425) - 2243963..2244292 (-) 330 WP_003131320.1 hypothetical protein -
  LLI11_RS11490 (LLI11_11430) - 2244289..2244633 (-) 345 WP_014570557.1 putative minor capsid protein -
  LLI11_RS11495 (LLI11_11435) - 2244623..2245024 (-) 402 WP_014570556.1 hypothetical protein -
  LLI11_RS11500 (LLI11_11440) - 2245098..2245334 (-) 237 WP_014570555.1 Ig-like domain-containing protein -
  LLI11_RS11505 (LLI11_11445) - 2245363..2246280 (-) 918 WP_003131315.1 hypothetical protein -
  LLI11_RS11510 (LLI11_11450) - 2246295..2247344 (-) 1050 WP_003131314.1 XkdF-like putative serine protease domain-containing protein -
  LLI11_RS11515 (LLI11_11455) - 2247360..2248190 (-) 831 WP_003131311.1 phage minor head protein -
  LLI11_RS11520 (LLI11_11460) - 2248183..2249712 (-) 1530 WP_058221397.1 phage portal protein -
  LLI11_RS11525 (LLI11_11465) terL 2249725..2251176 (-) 1452 WP_014570551.1 phage terminase large subunit -
  LLI11_RS11530 (LLI11_11470) - 2251157..2251630 (-) 474 WP_058221398.1 transposase -
  LLI11_RS11535 (LLI11_11475) - 2251801..2252190 (-) 390 WP_058221399.1 DUF722 domain-containing protein -
  LLI11_RS11540 (LLI11_11480) - 2252267..2252575 (-) 309 WP_082225258.1 hypothetical protein -
  LLI11_RS11545 (LLI11_11485) - 2252704..2252919 (-) 216 WP_058221541.1 DUF1660 domain-containing protein -
  LLI11_RS11550 (LLI11_11490) - 2252916..2253134 (-) 219 WP_014570803.1 hypothetical protein -
  LLI11_RS11555 (LLI11_11495) - 2253153..2253872 (-) 720 WP_082225259.1 hypothetical protein -
  LLI11_RS11560 (LLI11_11500) - 2253899..2254258 (-) 360 WP_082225260.1 hypothetical protein -
  LLI11_RS11565 (LLI11_11505) dut 2254262..2254681 (-) 420 WP_082225261.1 dUTP diphosphatase -
  LLI11_RS11570 (LLI11_13915) - 2254678..2255043 (-) 366 WP_082225262.1 DUF1642 domain-containing protein -
  LLI11_RS11575 (LLI11_11515) - 2255040..2255582 (-) 543 WP_145952586.1 DUF1642 domain-containing protein -
  LLI11_RS11580 (LLI11_13920) - 2255575..2255757 (-) 183 WP_243525495.1 hypothetical protein -
  LLI11_RS11585 (LLI11_13925) - 2255776..2255934 (-) 159 WP_228763273.1 hypothetical protein -
  LLI11_RS11590 (LLI11_11525) - 2256126..2256332 (-) 207 WP_014570535.1 hypothetical protein -
  LLI11_RS11595 (LLI11_11530) - 2256440..2256850 (-) 411 WP_014570810.1 hypothetical protein -
  LLI11_RS11600 (LLI11_13930) - 2256863..2257159 (-) 297 WP_284303385.1 L-rhamnose isomerase -
  LLI11_RS11605 (LLI11_11545) - 2257198..2258124 (-) 927 WP_058221710.1 phage replisome organizer N-terminal domain-containing protein -
  LLI11_RS11610 (LLI11_11550) - 2258387..2259454 (-) 1068 WP_058221711.1 DUF1351 domain-containing protein -
  LLI11_RS11615 (LLI11_11555) bet 2259456..2260193 (-) 738 WP_058212836.1 phage recombination protein Bet -
  LLI11_RS11620 (LLI11_11560) - 2260300..2260476 (-) 177 WP_032943269.1 putative transcriptional regulator -
  LLI11_RS11625 (LLI11_11565) - 2260473..2260721 (-) 249 WP_058221712.1 hypothetical protein -
  LLI11_RS11630 (LLI11_13935) - 2260734..2260856 (-) 123 WP_023164646.1 hypothetical protein -
  LLI11_RS11635 (LLI11_11570) - 2260853..2261035 (-) 183 WP_003130605.1 hypothetical protein -
  LLI11_RS11640 (LLI11_11575) - 2261051..2261740 (-) 690 WP_058221713.1 Rha family transcriptional regulator -
  LLI11_RS11645 (LLI11_11580) - 2261799..2262032 (-) 234 WP_014570819.1 helix-turn-helix transcriptional regulator -
  LLI11_RS11650 (LLI11_11585) - 2262209..2262619 (+) 411 WP_014570820.1 helix-turn-helix domain-containing protein -
  LLI11_RS11655 (LLI11_11590) - 2262630..2263214 (+) 585 WP_058221714.1 hypothetical protein -
  LLI11_RS11660 (LLI11_11595) - 2263270..2263809 (+) 540 WP_023189015.1 PH domain-containing protein -
  LLI11_RS11665 (LLI11_11600) - 2263935..2265392 (+) 1458 WP_058221720.1 recombinase family protein -
  LLI11_RS11670 (LLI11_11605) - 2265389..2265529 (-) 141 WP_023164653.1 hypothetical protein -
  LLI11_RS11675 (LLI11_11610) comGB 2265543..2266616 (-) 1074 WP_014570824.1 competence type IV pilus assembly protein ComGB Machinery gene

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40366.59 Da        Isoelectric Point: 10.4062

>NTDB_id=533119 LLI11_RS11675 WP_014570824.1 2265543..2266616(-) (comGB) [Lactococcus lactis subsp. lactis strain I.1.1]
MSLGHQKIGIKKLISYLQMDISHLLKPRGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKKFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=533119 LLI11_RS11675 WP_014570824.1 2265543..2266616(-) (comGB) [Lactococcus lactis subsp. lactis strain I.1.1]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAGAGGGAAAAAATTAAGGCTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAAAATTTACATTGAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTGGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACCTTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCATTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACATTTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

82.698

95.518

0.79
  comYB Streptococcus gordonii str. Challis substr. CH1

53.666

95.518

0.513
  comYB Streptococcus mutans UA140

53.372

95.518

0.51
  comYB Streptococcus mutans UA159

53.079

95.518

0.507
  comGB/cglB Streptococcus pneumoniae TIGR4

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae D39

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae R6

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae Rx1

51.343

93.838

0.482
  comGB/cglB Streptococcus mitis SK321

51.045

93.838

0.479
  comGB/cglB Streptococcus mitis NCTC 12261

50.149

93.838

0.471