Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   JNO50_RS16825 Genome accession   NZ_CP069161
Coordinates   3619819..3621528 (-) Length   569 a.a.
NCBI ID   WP_189531573.1    Uniprot ID   A0A918NZL4
Organism   Paludibacterium paludis strain BCRC 80514     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3614819..3626528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNO50_RS16795 - 3614865..3616109 (+) 1245 WP_189531563.1 N-acetylmuramoyl-L-alanine amidase -
  JNO50_RS16800 yacG 3616113..3616310 (-) 198 WP_189531565.1 DNA gyrase inhibitor YacG -
  JNO50_RS16805 zapD 3616319..3617077 (-) 759 WP_189531567.1 cell division protein ZapD -
  JNO50_RS16810 coaE 3617108..3617743 (-) 636 WP_189531569.1 dephospho-CoA kinase -
  JNO50_RS16815 - 3617733..3618605 (-) 873 WP_229804493.1 A24 family peptidase -
  JNO50_RS16820 pilC 3618602..3619822 (-) 1221 WP_189531571.1 type II secretion system F family protein Machinery gene
  JNO50_RS16825 pilF 3619819..3621528 (-) 1710 WP_189531573.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JNO50_RS16830 - 3621661..3622764 (-) 1104 WP_189531575.1 porin -
  JNO50_RS16835 - 3623221..3624294 (-) 1074 WP_189531577.1 porin -
  JNO50_RS16840 - 3624666..3626075 (+) 1410 WP_189531580.1 deoxyribodipyrimidine photo-lyase -
  JNO50_RS16845 - 3626072..3626386 (-) 315 WP_189531582.1 helix-hairpin-helix domain-containing protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62199.49 Da        Isoelectric Point: 5.4915

>NTDB_id=532560 JNO50_RS16825 WP_189531573.1 3619819..3621528(-) (pilF) [Paludibacterium paludis strain BCRC 80514]
MEGSQGVTGLGRALVQSRLLTREEAENIQRQAESSSVSFVEQLVLSRKKTAREIAEFAATLFGYPLFDLSAIEAESLPQG
LIDESVMVSRRVLPLFKRGNRLFVAASDPTQTSAFHAITFKTGLTVDVVVVEEDKLARVFSRRMSPGAESQGMPEIGGFD
ELGASDVTEETVQQEVDDAPVVRLIHKLLLDAIKSGASDIHFEPYEKYYRVRYRIDGELRLVAQPPLVLKEKIASRIKVV
SRLDIAEKRLPQDGRMKLSLSRDRAIDFRVSTLPTLFGEKIVMRILDAAAVSLDIDALGLNPIQKKLLTDAVARPWGMVL
VTGPTGSGKTVSLYTCLAMLNTPDINISTAEDPVEINLPGVNQVNVNEKAGLTFASALRAFLRQDPDVIMVGEIRDFETA
DIAIKAAQTGHRVFSTLHTNDAPSTLTRLLNMGIAPFNVASSVSLIMAQRLARKLCPSCRTPLDLPRETLLEAGFTEDEL
DGGWQSYGPAGCPECKGTGYRGRVGLFEVMPMTDAMTRLIMRNGNAVEIADLARKEGMIDLRRAGLIKVRQGETSLAEIE
AITSDGGRS

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=532560 JNO50_RS16825 WP_189531573.1 3619819..3621528(-) (pilF) [Paludibacterium paludis strain BCRC 80514]
ATGGAAGGATCGCAAGGTGTGACGGGTCTGGGCCGCGCTCTTGTTCAAAGCCGGTTGTTGACGCGAGAGGAAGCGGAAAA
CATTCAGCGGCAGGCCGAGTCGTCGTCGGTGAGCTTTGTCGAGCAACTGGTGTTGTCGCGCAAAAAAACCGCGCGCGAGA
TCGCCGAGTTCGCCGCCACGTTGTTCGGTTATCCGTTATTCGACCTGAGTGCCATCGAGGCGGAAAGTCTGCCGCAAGGC
TTGATCGACGAGAGCGTGATGGTGTCGCGCCGGGTTTTGCCTTTGTTCAAGCGTGGCAACCGGCTGTTCGTTGCGGCCTC
GGATCCGACGCAGACCTCGGCATTTCATGCCATTACCTTCAAAACCGGCCTGACAGTCGATGTGGTCGTGGTGGAAGAGG
ATAAGCTTGCCCGGGTATTCTCCCGTCGAATGTCGCCGGGAGCGGAAAGTCAGGGTATGCCGGAAATCGGTGGGTTCGAT
GAGCTTGGAGCCTCCGATGTCACCGAAGAAACGGTTCAACAGGAAGTCGATGATGCACCGGTGGTGCGTTTAATCCACAA
GCTGCTGCTGGATGCCATCAAATCCGGCGCTTCAGATATCCATTTCGAGCCCTACGAGAAATATTACCGCGTCCGCTATC
GCATCGATGGCGAGTTGCGCTTGGTTGCCCAGCCCCCCTTGGTGCTCAAGGAAAAAATCGCCTCCCGCATCAAGGTGGTG
TCGCGCCTGGATATCGCCGAGAAACGCTTGCCTCAGGACGGACGGATGAAGCTTTCCCTGTCGCGCGACAGGGCCATCGA
TTTCCGGGTGAGCACGCTGCCCACGCTGTTCGGCGAAAAAATCGTCATGCGTATCCTGGATGCGGCCGCCGTGTCCCTGG
ACATCGATGCGCTGGGTTTGAACCCGATCCAGAAAAAACTGCTGACGGACGCGGTGGCCCGCCCCTGGGGAATGGTGCTG
GTTACCGGCCCGACCGGGAGCGGAAAAACCGTATCGCTCTACACCTGTCTTGCCATGCTCAATACACCGGACATCAATAT
TTCCACCGCGGAGGATCCGGTAGAAATCAACTTGCCCGGCGTGAATCAGGTCAACGTCAATGAAAAGGCCGGATTGACCT
TCGCTTCCGCCCTTCGGGCTTTTTTGCGGCAGGATCCGGATGTGATCATGGTGGGGGAGATCCGGGATTTCGAAACCGCG
GATATCGCTATCAAGGCGGCCCAGACGGGGCATCGGGTTTTTTCCACCTTGCACACCAATGATGCGCCCTCGACCCTGAC
CCGTCTTTTGAATATGGGCATTGCGCCATTCAATGTGGCCAGCTCCGTATCGCTGATCATGGCGCAGCGGCTGGCCCGCA
AGCTGTGCCCATCATGCCGCACCCCCTTGGACCTTCCCCGTGAAACCTTGCTTGAAGCCGGATTCACAGAGGATGAACTG
GATGGTGGCTGGCAAAGCTATGGACCGGCTGGCTGTCCCGAATGCAAGGGAACCGGTTACCGCGGACGGGTGGGACTGTT
CGAAGTGATGCCCATGACCGACGCCATGACCCGGCTGATCATGCGCAACGGCAATGCCGTGGAAATCGCCGATCTGGCCC
GCAAGGAGGGCATGATCGATCTGCGCAGGGCAGGGTTGATCAAAGTGCGGCAGGGCGAAACGTCCCTTGCCGAAATCGAA
GCCATCACCAGCGATGGAGGTCGTTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

54.204

98.243

0.533
  pilB Acinetobacter baumannii D1279779

52.356

100

0.527
  pilB Acinetobacter baylyi ADP1

52.091

100

0.525
  pilB Legionella pneumophila strain ERS1305867

51.237

99.473

0.51
  pilB Vibrio cholerae strain A1552

49.468

99.121

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

48.313

98.946

0.478
  pilB Vibrio campbellii strain DS40M4

48.227

99.121

0.478
  pilF Thermus thermophilus HB27

39.076

98.946

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.011

98.946

0.376