Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   JJQ59_RS16440 Genome accession   NZ_CP068434
Coordinates   3547697..3549427 (+) Length   576 a.a.
NCBI ID   WP_114133588.1    Uniprot ID   -
Organism   Cupriavidus necator strain C39.     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3542697..3554427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJQ59_RS16410 (JJQ59_16410) obgE 3543399..3544496 (-) 1098 WP_114133585.1 GTPase ObgE -
  JJQ59_RS16415 (JJQ59_16415) rpmA 3544630..3544890 (-) 261 WP_010814747.1 50S ribosomal protein L27 -
  JJQ59_RS16420 (JJQ59_16420) rplU 3544930..3545241 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  JJQ59_RS16425 (JJQ59_16425) ispB 3545772..3546701 (+) 930 WP_114133586.1 octaprenyl diphosphate synthase -
  JJQ59_RS16435 (JJQ59_16435) - 3546980..3547300 (-) 321 WP_114133587.1 helix-turn-helix domain-containing protein -
  JJQ59_RS16440 (JJQ59_16440) pilF 3547697..3549427 (+) 1731 WP_114133588.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JJQ59_RS16445 (JJQ59_16445) - 3549493..3550764 (+) 1272 WP_114133589.1 type II secretion system F family protein -
  JJQ59_RS16450 (JJQ59_16450) pilD 3550768..3551670 (+) 903 WP_114133590.1 prepilin peptidase Machinery gene
  JJQ59_RS16455 (JJQ59_16455) coaE 3551734..3552360 (+) 627 WP_035826779.1 dephospho-CoA kinase -
  JJQ59_RS16460 (JJQ59_16460) zapD 3552570..3553328 (+) 759 WP_035826777.1 cell division protein ZapD -
  JJQ59_RS16465 (JJQ59_16465) - 3553346..3553534 (+) 189 WP_035826775.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 63570.22 Da        Isoelectric Point: 6.8828

>NTDB_id=529052 JJQ59_RS16440 WP_114133588.1 3547697..3549427(+) (pilF) [Cupriavidus necator strain C39.]
MTLGLALAQSRRIAPALLAQLEQAAREKQSQLIDEIVGSGTMSAHDLALFAADKYQLPLLDLAQYNLTKVPPALAGNREF
HAHRLLPLGRRENRLVLAMSDPSNQAGLDAIKEKYKLPVEAVVVEHDKLMKHVRSAGEALGTLKNISPVQAERKMIEYDP
VAAAGNQRNRTTADNIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLIALPKDKDAKETVERAVDFRVSTLPTLFGEKIVMRILESSSDKLDIDQLGYEPEQKALLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALRSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAAFNIASSVLMITAQRLARRLCTCKREGEGEIPREA
LLEAGFREQDLDGSWQPYHPVGCERCNGTGYKGRCGIYQVMPITEAMQEIILTHGTALQIAEQARKDGVLSLREAGLLKV
KQGVTSLEEVLATTNT

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=529052 JJQ59_RS16440 WP_114133588.1 3547697..3549427(+) (pilF) [Cupriavidus necator strain C39.]
ATGACACTCGGTCTTGCCCTGGCCCAGAGCCGGCGTATCGCGCCCGCCCTGCTTGCTCAGCTGGAGCAGGCCGCGCGTGA
AAAGCAGTCGCAGCTGATCGACGAGATCGTCGGCAGCGGCACCATGAGCGCGCACGACCTGGCGCTGTTTGCCGCGGACA
AGTACCAGCTGCCGCTGCTGGACCTGGCCCAGTACAACCTGACCAAGGTGCCGCCGGCGCTGGCCGGCAACCGTGAATTC
CACGCGCACCGGCTGCTGCCGCTGGGCCGGCGCGAGAACCGGCTGGTGCTGGCGATGTCCGACCCGTCCAACCAGGCCGG
GCTGGATGCGATCAAGGAAAAGTACAAGCTGCCGGTCGAAGCGGTGGTGGTCGAGCACGACAAGCTGATGAAGCACGTGC
GCTCCGCCGGCGAGGCCCTGGGCACGCTCAAGAACATCTCGCCGGTGCAGGCTGAGCGCAAGATGATCGAATACGATCCG
GTGGCCGCGGCCGGCAACCAGCGCAACCGCACCACCGCCGACAATATCGACGACGCCCCGGTGGTGCGCTTCCTGCAGAA
GCTGCTGACCGAGGCCTTCCATCGCGGCGCATCCGACCTGCACTTCGAGCCGTTTGAGACCTTCTACCGCATCCGTTTCC
GCGTGGACGGGGTGCTGCAGGAGGTTGCGCGCCCGCCGCTGGATATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTG
TCGCGCCTGGATATTTCTGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATTGCCCTGCCCAAGGACAAGGA
CGCCAAGGAGACGGTCGAGCGGGCAGTGGATTTCCGCGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATGC
GGATCCTGGAATCTTCGTCCGACAAGCTCGACATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGGCGCTGCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGCAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACCTGCTGAACCAGGGCGACATCAATATCTCCACCGCGGAAGACCCGGCCGAAATCCAGCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGCCTGACCTTTGCCGCGGCCCTGCGTTCGTTCCTGCGGCAGGATCCGGACATCATCATG
GTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACCGGCCACCTGGTGTTGTCGACGCT
GCACACCAACGATGCGCCGACCACGCTGACGCGGTTGATGAACATGGGCGTGGCGGCCTTCAATATTGCGTCGAGCGTGC
TGATGATCACCGCGCAGCGGCTGGCGCGGCGGCTGTGCACCTGCAAGCGCGAGGGCGAGGGCGAGATCCCGCGCGAGGCG
CTGCTGGAGGCGGGCTTCCGCGAACAAGACCTGGACGGCAGCTGGCAGCCCTACCACCCGGTCGGCTGCGAGCGCTGCAA
CGGCACCGGCTACAAGGGCCGCTGCGGCATCTACCAGGTCATGCCGATCACCGAGGCCATGCAGGAGATCATCCTGACGC
ATGGCACGGCACTGCAGATCGCCGAGCAGGCGCGCAAGGATGGCGTGCTATCGTTGCGCGAAGCGGGGCTGCTGAAGGTG
AAGCAGGGCGTCACGTCACTCGAAGAAGTGCTGGCGACAACGAATACTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)

Type II/IV secretion system protein (T2SSE)

(187-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

51.724

100

0.521
  pilB Acinetobacter baumannii D1279779

52.014

99.132

0.516
  pilB Acinetobacter baylyi ADP1

51.885

96.701

0.502
  pilB Legionella pneumophila strain ERS1305867

49.909

95.66

0.477
  pilB Vibrio cholerae strain A1552

45.009

99.132

0.446
  pilB Vibrio parahaemolyticus RIMD 2210633

46.026

93.924

0.432
  pilB Vibrio campbellii strain DS40M4

45.856

94.271

0.432
  pilF Thermus thermophilus HB27

38.86

100

0.391
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.138

96.701

0.378