Detailed information NTG0529053

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: JJQ59_RS16450
• Genome accession: NZ_CP068434
• Coordinates: 3550768..3551670 (+)
• Length: 300 a.a.
• NCBI ID: WP_114133590.1
• Uniprot ID: A0A367PFD2
• Organism: Cupriavidus necator strain C39.
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3545768..3556670

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JJQ59_RS16425 (JJQ59_16425)	ispB	3545772..3546701 (+)	930	WP_114133586.1	octaprenyl diphosphate synthase	-
JJQ59_RS16435 (JJQ59_16435)	-	3546980..3547300 (-)	321	WP_114133587.1	helix-turn-helix domain-containing protein	-
JJQ59_RS16440 (JJQ59_16440)	pilF	3547697..3549427 (+)	1731	WP_114133588.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JJQ59_RS16445 (JJQ59_16445)	-	3549493..3550764 (+)	1272	WP_114133589.1	type II secretion system F family protein	-
JJQ59_RS16450 (JJQ59_16450)	pilD	3550768..3551670 (+)	903	WP_114133590.1	prepilin peptidase	Machinery gene
JJQ59_RS16455 (JJQ59_16455)	coaE	3551734..3552360 (+)	627	WP_035826779.1	dephospho-CoA kinase	-
JJQ59_RS16460 (JJQ59_16460)	zapD	3552570..3553328 (+)	759	WP_035826777.1	cell division protein ZapD	-
JJQ59_RS16465 (JJQ59_16465)	-	3553346..3553534 (+)	189	WP_035826775.1	DNA gyrase inhibitor YacG	-
JJQ59_RS16470 (JJQ59_16470)	-	3553565..3555604 (-)	2040	WP_114133591.1	OPT family oligopeptide transporter	-
JJQ59_RS16475 (JJQ59_16475)	-	3555688..3556134 (-)	447	WP_114133592.1	NUDIX domain-containing protein	-

Sequence

Protein

• Length: 300 a.a.
• Molecular weight: 32053.23 Da
• Isoelectric Point: 8.0221

>NTDB_id=529053 JJQ59_RS16450 WP_114133590.1 3550768..3551670(+) (pilD) [Cupriavidus necator strain C39.]
MLSAWSASPYPTGGAPLVEALAALPPAFLVAAAALLGLVVGSFLNVVIHRMPRMMERDEANYIAELRGDPLPYPGRYNLM
VPRSACPHCGHAIAPWENVPVLSYLFLRGRCSACKAPISVRYPLVELACGVLSALAAWRFGPGGQALAALVLVWALLALT
MIDADTQLLPDQITLPLLWLGLLLNLGGVFVALPDAVIGAAAGYLVLWTAYWLFRLVRGKEGMGFGDFKLMAALGAWFGW
QALPALVLLSSVVGVLFGLANIALRRQERDTPFPFGPFIALAGVVVLLFGPGVLPLFAWP

Nucleotide

• Length: 903 bp

>NTDB_id=529053 JJQ59_RS16450 WP_114133590.1 3550768..3551670(+) (pilD) [Cupriavidus necator strain C39.]
ATGTTGTCTGCGTGGTCTGCTTCTCCGTATCCGACCGGTGGCGCGCCGCTGGTGGAGGCACTGGCGGCGCTGCCGCCCGC
CTTTCTGGTGGCTGCCGCGGCGCTGCTCGGGCTGGTGGTCGGCAGCTTCCTCAACGTGGTCATCCACCGGATGCCGCGCA
TGATGGAGCGCGACGAGGCCAACTATATCGCCGAGCTGCGCGGCGACCCGCTGCCCTACCCCGGCCGCTACAACCTGATG
GTGCCGCGCTCGGCCTGCCCGCATTGCGGCCACGCGATTGCACCGTGGGAGAACGTGCCGGTGCTGAGCTACCTGTTCCT
GCGCGGGCGCTGCTCGGCATGCAAGGCGCCGATCAGTGTGCGCTATCCGCTGGTCGAGCTGGCCTGTGGCGTGCTGAGCG
CGCTGGCGGCGTGGCGCTTCGGCCCCGGCGGACAGGCCCTGGCCGCGCTGGTGTTGGTGTGGGCACTGCTGGCGCTGACC
ATGATCGATGCCGATACGCAACTGTTGCCGGACCAGATCACCCTGCCGCTGCTGTGGCTGGGCCTGCTGCTGAACCTGGG
CGGCGTGTTTGTGGCGCTGCCCGACGCCGTGATCGGCGCGGCTGCCGGCTACCTGGTGCTGTGGACGGCCTACTGGCTGT
TCCGGCTCGTGCGCGGCAAGGAAGGCATGGGCTTTGGCGACTTCAAGCTGATGGCGGCGCTGGGCGCCTGGTTCGGCTGG
CAGGCGCTGCCGGCGCTGGTGCTGCTGTCGTCGGTGGTCGGCGTGCTGTTCGGGCTGGCCAATATTGCCCTGCGCCGCCA
GGAACGCGACACGCCCTTCCCGTTCGGGCCTTTCATCGCGCTGGCCGGCGTGGTGGTGCTGCTGTTCGGCCCCGGCGTGC
TGCCGCTGTTTGCATGGCCATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (35-140)

• Type IV leader peptidase family (Peptidase_A24) (150-259)

3D structure

• AlphaFold DB: A0A367PFD2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.849	88.333	0.493
pilD	Vibrio campbellii strain DS40M4	51.273	91.667	0.47
pilD	Neisseria gonorrhoeae MS11	47.183	94.667	0.447
pilD	Acinetobacter baumannii D1279779	47.232	90.333	0.427
pilD	Acinetobacter nosocomialis M2	46.863	90.333	0.423