Detailed information NTG0527884

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I6I51_RS02555
• Genome accession: NZ_CP068187
• Coordinates: 458707..459900 (+)
• Length: 397 a.a.
• NCBI ID: WP_201703229.1
• Uniprot ID: A0AA42MTU9
• Organism: Acinetobacter johnsonii strain FDAARGOS_1094
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 453707..464900

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6I51_RS02540 (I6I51_02545)	-	454817..456916 (+)	2100	WP_201703219.1	EAL domain-containing protein	-
I6I51_RS17760 (I6I51_02550)	-	457316..457462 (-)	147	WP_171254088.1	hypothetical protein	-
I6I51_RS02550 (I6I51_02555)	-	457408..458469 (+)	1062	WP_201703227.1	3-deoxy-7-phosphoheptulonate synthase	-
I6I51_RS02555 (I6I51_02560)	pilU	458707..459900 (+)	1194	WP_201703229.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I6I51_RS02560 (I6I51_02565)	-	459948..461126 (-)	1179	WP_201703231.1	MFS transporter	-
I6I51_RS02565 (I6I51_02570)	gcvH	461387..461761 (-)	375	WP_201703233.1	glycine cleavage system protein GcvH	-
I6I51_RS02570 (I6I51_02575)	-	461833..462861 (-)	1029	WP_004979806.1	AraC family transcriptional regulator	-

Sequence

Protein

• Length: 397 a.a.
• Molecular weight: 44690.11 Da
• Isoelectric Point: 6.5063

>NTDB_id=527884 I6I51_RS02555 WP_201703229.1 458707..459900(+) (pilU) [Acinetobacter johnsonii strain FDAARGOS_1094]
MYSAELLEEARRMMFHMLSKVVEYGGSDLFISADFPPSIKHQGLMKPLGQQNLPSDQTKLFAYSLMNEKQRLEFETELEC
NFAISVPNVSRFRVNVFQQQLHVGMVIRTITAEIPNFTKLQLPTSLKDVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPDERRNQLLMDLSSNMKAIISQRLVRTEDGRGRRAAVEIMLNTPLMSDLILKGNFHELKEVMSKS
RELGMQTFDQALFDLYNQGAIAYEEALRNADSVNELRLQIKLKSSRANPQLSSNSALSFDQAIAEKAKDAEEEKVDA

Nucleotide

• Length: 1194 bp

>NTDB_id=527884 I6I51_RS02555 WP_201703229.1 458707..459900(+) (pilU) [Acinetobacter johnsonii strain FDAARGOS_1094]
ATGTATTCTGCAGAACTTTTAGAAGAAGCCCGTCGGATGATGTTTCACATGCTCAGCAAAGTGGTTGAGTATGGTGGTTC
AGACTTATTTATATCTGCAGACTTTCCGCCAAGTATTAAGCATCAGGGCCTTATGAAGCCATTGGGTCAACAAAACTTGC
CATCTGATCAGACCAAACTCTTTGCTTATAGTTTAATGAACGAAAAGCAACGATTAGAGTTCGAAACTGAGCTGGAGTGT
AATTTTGCAATTAGCGTTCCCAATGTCTCACGTTTTCGCGTCAATGTTTTTCAGCAGCAACTGCATGTAGGTATGGTGAT
TCGTACGATTACTGCGGAAATTCCTAACTTCACTAAGTTACAACTTCCAACGTCTTTAAAAGACGTAATTATGGAAAAAC
GTGGTTTGGTCCTCGTTGTCGGTGGTACAGGGTCGGGTAAATCGACGTCTTTGGCAGCCATGATTGATCATCGAAATGAA
AACTCGGCAGGGCACATTATTACGGTTGAAGATCCTGTGGAGTATGTACATAAACATAAGAAATCGATGATTACTCATCG
AGAAGTGGGTGTGGACTGTCACTCTTGGCACAATGCTTTGAAAAATACTTTGCGCCAAGCGCCCGATGTCATTTTAATTG
GTGAGATTCGTGACACGGAGACTATGGAGCATGCCATAGCTTTTGCAGAAACAGGCCACTTATGCCTAGGCACATTACAT
GCTAATAATGCAAATCAGGCTTTAGATCGAATTATTAACTTTTTCCCAGACGAACGTCGCAATCAACTTTTGATGGACTT
ATCTTCTAATATGAAGGCGATAATTTCACAGCGTCTGGTGCGAACTGAAGATGGGCGAGGTCGCCGAGCAGCAGTGGAAA
TCATGCTCAATACACCGTTGATGTCAGACTTGATACTTAAAGGGAATTTCCATGAACTCAAAGAAGTGATGTCGAAATCA
CGAGAGCTCGGTATGCAGACTTTTGATCAGGCTTTGTTTGATTTATACAATCAAGGTGCAATCGCTTATGAAGAAGCACT
ACGAAATGCAGACTCGGTCAATGAATTACGTCTACAGATTAAGTTAAAGAGTAGTCGTGCCAACCCGCAGTTAAGCTCAA
ATAGTGCTTTGTCTTTTGATCAAGCGATTGCTGAAAAAGCCAAAGACGCTGAAGAAGAGAAGGTCGACGCCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (122-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.331	90.428	0.537
pilU	Acinetobacter baylyi ADP1	54.821	91.436	0.501
pilU	Vibrio cholerae strain A1552	50.578	87.154	0.441
pilT	Legionella pneumophila strain Lp02	42.478	85.39	0.363
pilT	Legionella pneumophila strain ERS1305867	42.478	85.39	0.363
pilT	Acinetobacter baylyi ADP1	43.598	82.62	0.36