Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JJL53_RS20270 Genome accession   NZ_CP067417
Coordinates   4370678..4372384 (+) Length   568 a.a.
NCBI ID   WP_139751891.1    Uniprot ID   -
Organism   Aeromonas media strain E31     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 4369042..4370178 4370678..4372384 flank 500


Gene organization within MGE regions


Location: 4369042..4372384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJL53_RS20260 (JJL53_20260) - 4369042..4370178 (+) 1137 WP_041204891.1 ISAs1-like element ISAeme1 family transposase -
  JJL53_RS25195 (JJL53_20265) - 4370224..4370637 (+) 414 WP_081805615.1 hypothetical protein -
  JJL53_RS20270 (JJL53_20270) pilB 4370678..4372384 (+) 1707 WP_139751891.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62627.00 Da        Isoelectric Point: 5.8214

>NTDB_id=526026 JJL53_RS20270 WP_139751891.1 4370678..4372384(+) (pilB) [Aeromonas media strain E31]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLSEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEEGKLTAAIGKLMESDQDTLGMDHIDEAEI
SELEVSDESSRLDDSVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKVVIRLLDSSASRLNLDQLGFDERQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKVPEAVPEAELLEFGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=526026 JJL53_RS20270 WP_139751891.1 4370678..4372384(+) (pilB) [Aeromonas media strain E31]
ATGACATCTAGCCTCAATAGCGGCCTGGCCACCAGCTTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTCACAACGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAACCCTTTGTCACCTTCCTGATCGAAAATGACATCCTCGACAGCAAGGCCCTCG
CGGATTTCTGCGAGCTGGAGTATGGAGTACCTCTGCTGGATCTTGCCGCCTTCGACCTCTCCGAAATCCCGCAGAAGTAC
CTCAATCAGAAATTGATCGAAAAGCACCACGTCCTGCCCATCTACACCCAAGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTATCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGCCTGCACACCGAGGCCTTGCTGGTTGAAGAGGGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAAAGCGATCAGGATACCCTGGGCATGGATCACATCGACGAGGCCGAAATC
TCGGAACTCGAGGTCTCCGATGAGAGTTCGCGACTCGACGACAGCGTCAACACTGCCGACGACGATGCCCCCATCGTCAA
ATACATTCACAAGATCATGATGGACGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACC
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCTCGC
TTAAAGGTAATGGCGCGGCTAGATATCGCCGAGCGGCGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCCCGCAA
CCGGTCCATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAAAAGGTTGTCATTCGCCTGCTGGACTCGTCAG
CCTCTCGCCTCAACCTCGATCAACTCGGCTTCGACGAACGCCAGAAGGCGCAATATCTGCATGCGCTCTCCAAACCACAG
GGCATGATACTGGTCACGGGCCCCACGGGATCGGGCAAGACGGTGTCACTCTATACCGGTCTCAACATCCTCAATACCAG
CGAGGTCAACATCTCCACCGCTGAGGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCTCAGACCGGACACCTGGTACTGTCAACTCTGCACACCAACTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GCCTCGCCCGCAAGCTGTGCGATCACTGCAAGGTGCCCGAGGCAGTGCCGGAAGCCGAATTGCTGGAGTTCGGTTTTACC
CAGCAACAGCTGGCCGCCGGTTTGCGGCTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCGGGAGGATACAGAGGACGGGT
CGGCATCTATGAAATCATGCTGATGTCGGAGAACATCGCCAAGCTGATCATGCAGGGAGCAAACTCGCTGCAGATCGCTG
CCATCGCCCAGAAAGAGGGTATGCGTACCCTGCGCATTTCCGGCCTGGAGAAGGCCCGTCTGGGGGTCACCAGCCTGGCC
GAGATCAACAGGATCACGACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.674

99.824

0.546
  pilB Acinetobacter baylyi ADP1

54.529

99.12

0.54
  pilB Legionella pneumophila strain ERS1305867

54.078

99.296

0.537
  pilB Vibrio cholerae strain A1552

53.014

99.296

0.526
  pilB Vibrio parahaemolyticus RIMD 2210633

49.378

99.12

0.489
  pilB Vibrio campbellii strain DS40M4

49.378

99.12

0.489
  pilF Neisseria gonorrhoeae MS11

46.208

99.824

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.225

97.183

0.371
  pilF Thermus thermophilus HB27

39.736

93.486

0.371