Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JGR68_RS02900 Genome accession   NZ_CP067113
Coordinates   620809..621939 (+) Length   376 a.a.
NCBI ID   WP_199360375.1    Uniprot ID   -
Organism   Luteimonas sp. MC1750     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 615809..626939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JGR68_RS02870 (JGR68_02870) pdxH 616068..616664 (+) 597 WP_199360365.1 pyridoxamine 5'-phosphate oxidase -
  JGR68_RS02875 (JGR68_02875) - 616661..617494 (+) 834 WP_234446569.1 ABC transporter substrate-binding protein -
  JGR68_RS02880 (JGR68_02880) - 617589..617984 (+) 396 WP_199360367.1 DoxX family protein -
  JGR68_RS02885 (JGR68_02885) proC 617993..618832 (-) 840 WP_199360369.1 pyrroline-5-carboxylate reductase -
  JGR68_RS02890 (JGR68_02890) - 618926..619624 (-) 699 WP_199360371.1 YggS family pyridoxal phosphate-dependent enzyme -
  JGR68_RS02895 (JGR68_02895) pilT 619759..620796 (+) 1038 WP_199360373.1 type IV pilus twitching motility protein PilT Machinery gene
  JGR68_RS02900 (JGR68_02900) pilU 620809..621939 (+) 1131 WP_199360375.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JGR68_RS02905 (JGR68_02905) - 622054..622680 (+) 627 WP_199360377.1 superoxide dismutase -
  JGR68_RS02910 (JGR68_02910) - 622887..623348 (+) 462 WP_199360379.1 peptidylprolyl isomerase -
  JGR68_RS02915 (JGR68_02915) - 623355..625154 (-) 1800 WP_199360381.1 DUF4153 domain-containing protein -
  JGR68_RS02920 (JGR68_02920) - 625234..625800 (+) 567 WP_199360385.1 YqgE/AlgH family protein -
  JGR68_RS02925 (JGR68_02925) ruvX 625800..626288 (+) 489 WP_199360387.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41834.13 Da        Isoelectric Point: 6.6610

>NTDB_id=525109 JGR68_RS02900 WP_199360375.1 620809..621939(+) (pilU) [Luteimonas sp. MC1750]
MSAIDFTSYLKLMAHQKASDLFITAGMPPSMKVHGKISPITQNPLTPQQARDLVLNVMTPPQREEFERTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTIEELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLASMIGYRNANSTGHIIT
IEDPIEFVHKHDGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRGQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSANEVRLRIKLAQGGDARTLSQGLDGVEVAEVT

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=525109 JGR68_RS02900 WP_199360375.1 620809..621939(+) (pilU) [Luteimonas sp. MC1750]
ATGAGCGCCATCGACTTCACCTCGTACCTCAAGCTGATGGCCCACCAGAAGGCCTCGGACCTGTTCATCACCGCCGGCAT
GCCGCCGTCGATGAAGGTCCACGGCAAGATCTCGCCGATCACCCAGAACCCGCTGACGCCGCAGCAGGCGCGCGACCTGG
TGCTCAACGTGATGACGCCGCCGCAGCGCGAGGAGTTCGAGCGCACGCACGAGTGCAACTTCGCGATCGGCGTGTCCGGC
GTGGGCCGCTTCCGCGTGTCGTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACCCGCATCCC
GACGATCGAGGAGCTCAACCTCCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTGGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGTCGATGATCGGCTACCGCAACGCCAACTCGACCGGGCACATCATCACC
ATCGAGGATCCGATCGAGTTCGTGCACAAGCACGACGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTCCGCACCCGCGAGGGCA
TGGACCACGCCATCGCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGGCGCGGCCAGCTGCTGATGGACCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATCCCGACCCCGGACGGCAAGGGCCGCCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAATCATGAAGGAGTCGACCAACCTCGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCTCCTACGAGGACGCGCTGCGCTATGCCGACTCGGCCAACGA
GGTGCGCCTGCGCATCAAGCTGGCGCAGGGCGGCGACGCGCGCACGCTGTCGCAGGGCCTGGACGGCGTCGAGGTGGCCG
AGGTCACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.12

97.606

0.665
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.109

90.691

0.391
  pilT Pseudomonas aeruginosa PAK

40.597

89.096

0.362