Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JH259_RS06060 Genome accession   NZ_CP067005
Coordinates   1428011..1429717 (+) Length   568 a.a.
NCBI ID   WP_181920283.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain 54-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1423011..1434717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH259_RS06035 (JH259_06015) - 1423299..1423700 (-) 402 WP_267314850.1 SymE family type I addiction module toxin -
  JH259_RS06040 (JH259_06020) coaE 1424232..1424855 (-) 624 WP_040940796.1 dephospho-CoA kinase -
  JH259_RS06045 (JH259_06025) - 1424869..1425732 (-) 864 WP_267314851.1 A24 family peptidase -
  JH259_RS06050 (JH259_06030) pilC 1425739..1426998 (-) 1260 WP_070690003.1 type II secretion system F family protein Machinery gene
  JH259_RS06055 (JH259_06035) pilA2 1427349..1427768 (+) 420 WP_116670568.1 pilin Machinery gene
  JH259_RS06060 (JH259_06040) pilB 1428011..1429717 (+) 1707 WP_181920283.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH259_RS06065 (JH259_06045) - 1429900..1430637 (-) 738 WP_070690009.1 zeta toxin family protein -
  JH259_RS06070 (JH259_06050) - 1430637..1430993 (-) 357 WP_139328384.1 hypothetical protein -
  JH259_RS06075 (JH259_06055) pilR 1431191..1432585 (-) 1395 WP_267314854.1 sigma-54 dependent transcriptional regulator Regulator
  JH259_RS06080 (JH259_06060) - 1432795..1434405 (-) 1611 WP_040940804.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 61913.08 Da        Isoelectric Point: 5.6039

>NTDB_id=524164 JH259_RS06060 WP_181920283.1 1428011..1429717(+) (pilB) [Xanthomonas campestris pv. campestris strain 54-3]
MGITGIARRLVQDGVLDEGSARAAMDQAALAKVPLPQWFAEKKLVSAAQLAAANAIEFGMPLMDVSVFDVNQNAVKLVSE
ELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQAGNASFGSVLGGDDEEMGDL
DVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLNQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQKLFLDAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPEHALLAEGF
TPEQVANIDLYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMKASHGVTSLA
EINRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=524164 JH259_RS06060 WP_181920283.1 1428011..1429717(+) (pilB) [Xanthomonas campestris pv. campestris strain 54-3]
GTGGGTATCACAGGAATAGCACGGCGTTTGGTTCAAGACGGCGTACTTGATGAAGGGAGCGCTCGCGCTGCGATGGATCA
GGCAGCATTGGCGAAAGTGCCGCTTCCTCAGTGGTTCGCGGAAAAGAAGCTTGTGTCAGCTGCTCAACTCGCAGCTGCTA
ACGCAATCGAGTTTGGCATGCCGTTGATGGACGTGTCTGTTTTCGACGTCAACCAGAACGCAGTCAAGCTGGTCAGTGAG
GAGTTGCTTCAGAAATACCAGGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTTGTTCGTGGGGGTGAGCAACCCGACCCA
GACGCGTGCGCTGGACGACATCAAGTTCCACACCAATCTGGTTGTCGAGCCGATTTTGGTCGATGAAGATCAGATCCGTA
GGACGTTGGAGCAATGGCAGGCAGGAAACGCGTCCTTTGGCTCGGTGCTTGGTGGCGACGACGAGGAGATGGGGGACCTA
GACGTGTCCGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGATGACACGCCGGTGGT
GAAATTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACATCCATTTCGAGCCCTATGAAGACGACT
ACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCGGTGAAGCTCAACCAGCGCATCGCGGCG
CGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGCATCAAGCTCAATCTGTCCAA
GACCAAGCAGATCGACTTTCGCGTGAGCACCTTGCCGACCCTGTTCGGCGAGAAGGTGGTGTTGCGTATCCTGGACGCCA
GTGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCGGACCAGCAGAAGCTGTTCCTGGATGCGATCCACAAGCCC
TACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCTGTATACCGCGCTCGGGATTCTCAACGA
CGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGCCCGGCGTCAATCAGGTGCAGCAGAACGTCA
AGCGGGGCATGACGTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCGGACATCATCATGGTCGGCGAAATCCGC
GACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGCCACATGGTGCTGTCGACGTTGCACACCAACGATGC
GCCGCAGACCATTGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCACCTCGTCGGTGACCCTGGTCATCGCGC
AGCGCTTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCGGAACATGCGCTGCTGGCGGAAGGCTTT
ACGCCGGAGCAGGTTGCGAACATCGACCTCTATGAAGCGGTCGGTTGCGATGAGTGCACGGAAGGCTACAAGGGCCGTAC
CGGCATCTATCAGGTGATGCCGATGACGGACGAAATCGGCGCGATCGTGCTGGAAGGCGGCAATGCGATGCAGATCGCCG
AGGCTGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTAATGAAGGCTTCGCACGGCGTGACCAGCCTGGCC
GAAATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

100

0.562
  pilB Acinetobacter baylyi ADP1

55.81

100

0.558
  pilB Legionella pneumophila strain ERS1305867

52.465

100

0.525
  pilB Vibrio cholerae strain A1552

48.951

100

0.493
  pilF Neisseria gonorrhoeae MS11

49.12

100

0.491
  pilB Vibrio campbellii strain DS40M4

45.694

100

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

45.745

99.296

0.454
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.313

92.254

0.363
  pilF Thermus thermophilus HB27

39.499

91.373

0.361