Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JH305_RS16520 Genome accession   NZ_CP066988
Coordinates   3790308..3792014 (-) Length   568 a.a.
NCBI ID   WP_274400977.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain 42-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3785308..3797014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH305_RS16510 (JH305_16445) - 3786677..3788287 (+) 1611 WP_011038210.1 HAMP domain-containing sensor histidine kinase -
  JH305_RS16515 (JH305_16450) pilR 3788496..3789890 (+) 1395 WP_042594919.1 sigma-54 dependent transcriptional regulator Regulator
  JH305_RS16520 (JH305_16455) pilB 3790308..3792014 (-) 1707 WP_274400977.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH305_RS16525 (JH305_16460) pilA2 3792289..3792699 (-) 411 WP_228424134.1 pilin Machinery gene
  JH305_RS16530 (JH305_16465) pilC 3793051..3794310 (+) 1260 WP_228424132.1 type II secretion system F family protein Machinery gene
  JH305_RS16535 (JH305_16470) - 3794317..3795180 (+) 864 WP_228876553.1 A24 family peptidase -
  JH305_RS16540 (JH305_16475) coaE 3795194..3795817 (+) 624 WP_274398949.1 dephospho-CoA kinase -
  JH305_RS16545 (JH305_16480) - 3796349..3796750 (+) 402 WP_040940795.1 SymE family type I addiction module toxin -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62133.24 Da        Isoelectric Point: 5.4530

>NTDB_id=523949 JH305_RS16520 WP_274400977.1 3790308..3792014(-) (pilB) [Xanthomonas campestris pv. campestris strain 42-2]
MGITGIARRLVQDGVLDETMARSAMEQASIAKVPLPQWFAEKKLVTGAQLAAANAVEFGMPLLDMSAFDASQNAVKLVSE
ELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQANSASFGSSMGDDDEEMGDL
DVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLNQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQKLFLDAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPEHALLAEGF
TQEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMKASHGVTSLA
EINRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=523949 JH305_RS16520 WP_274400977.1 3790308..3792014(-) (pilB) [Xanthomonas campestris pv. campestris strain 42-2]
GTGGGTATTACCGGGATCGCACGACGTCTGGTCCAAGATGGCGTGCTGGACGAAACAATGGCGCGTTCTGCCATGGAGCA
GGCTTCTATAGCCAAAGTCCCGCTTCCTCAATGGTTTGCAGAGAAAAAATTAGTCACAGGCGCACAACTCGCCGCTGCAA
ACGCAGTGGAGTTTGGTATGCCTCTGCTGGATATGTCTGCGTTCGATGCCAGCCAGAATGCAGTCAAGCTTGTTAGTGAA
GAGTTGCTCCAAAAATATCAGGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTTGTTCGTGGGGGTGAGCAACCCGACCCA
GACGCGTGCGCTGGACGACATCAAGTTCCACACCAATCTGGTTGTCGAGCCGATTTTGGTCGATGAAGATCAGATTCGTC
GCACATTGGAGCAATGGCAGGCCAATAGCGCTTCGTTTGGCTCGTCAATGGGTGACGATGACGAAGAAATGGGCGATCTG
GACGTCTCCACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGATGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGATATCCACTTCGAGCCGTATGAAGACGACT
ACCGTGTGCGGTTGCGGATCGATGGGTTGTTGAAGAACGTAGCCAAGGCGCCGGTGAAGCTCAACCAGCGCATCGCGGCG
CGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGCATCAAACTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCGACCTTGTTCGGCGAGAAGGTGGTGCTGCGTATCCTGGACGCCA
GTGCGGCCAAGCTCGGTATCGAGAAGCTGGGTTACGAGGCGGATCAGCAGAAGCTGTTCCTGGATGCGATCCACAAGCCC
TACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCTGTATACCGCGCTCGGGATTCTCAACGA
CGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCCTGCCTGGCGTCAATCAGGTGCAGCAGAACGTCA
AGCGGGGCATGACGTTTGCCGCTGCGCTGCGCTCTTTCCTGCGTCAGGACCCGGACATCATCATGGTCGGCGAAATCCGC
GACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTCGACGCTGCACACCAACGATGC
GCCGCAAACCATTGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACATTACCTCATCGGTGACCCTGGTCATCGCGC
AGCGCCTGGCGCGGCGGCTGTGCAACAACTGTAAGCGCAAGTCGACGCTGCCGGAACATGCGCTGCTGGCGGAAGGCTTT
ACGCAGGAGCAGGTTGCGAACATCGAGCTCTATGAGGCGGTCGGTTGCGATGAATGCACGGAAGGCTACAAGGGCCGCAC
CGGTATCTATCAGGTGATGCCGATGACCGATGAAATCGGCGCGATCGTGCTGGAAGGCGGCAATGCGATGCAGATCGCCG
AGGCCGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAGGCTTCGCACGGGGTGACCAGCCTGGCT
GAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-143)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.81

100

0.558
  pilB Acinetobacter baumannii D1279779

55.458

100

0.555
  pilB Legionella pneumophila strain ERS1305867

51.937

100

0.519
  pilF Neisseria gonorrhoeae MS11

49.12

100

0.491
  pilB Vibrio cholerae strain A1552

48.501

99.824

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

46.774

98.239

0.46
  pilB Vibrio campbellii strain DS40M4

45.694

100

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.961

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.208

96.303

0.368