Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JH273_RS16750 Genome accession   NZ_CP066963
Coordinates   3843600..3845333 (+) Length   577 a.a.
NCBI ID   WP_274507695.1    Uniprot ID   -
Organism   Xanthomonas campestris strain 3054     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3838600..3850333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH273_RS16725 (JH273_16700) - 3839874..3840290 (-) 417 WP_228441075.1 pilin -
  JH273_RS16730 (JH273_16705) - 3840515..3841015 (+) 501 WP_274507692.1 EamA family transporter -
  JH273_RS16735 (JH273_16710) - 3841020..3841814 (+) 795 WP_228441069.1 glycosyltransferase family 2 protein -
  JH273_RS16740 (JH273_16715) - 3841811..3842839 (+) 1029 WP_274507693.1 glycosyltransferase -
  JH273_RS16745 (JH273_16720) - 3842850..3843449 (+) 600 WP_228441064.1 methionine biosynthesis protein MetW -
  JH273_RS16750 (JH273_16725) pilB 3843600..3845333 (+) 1734 WP_274507695.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH273_RS16755 (JH273_16730) - 3845516..3846253 (-) 738 WP_274339071.1 zeta toxin family protein -
  JH273_RS16760 (JH273_16735) - 3846253..3846609 (-) 357 WP_139328384.1 hypothetical protein -
  JH273_RS16765 (JH273_16740) pilR 3846807..3848201 (-) 1395 WP_274507697.1 sigma-54 dependent transcriptional regulator Regulator
  JH273_RS16770 (JH273_16745) - 3848410..3850020 (-) 1611 WP_116901781.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62833.90 Da        Isoelectric Point: 5.4486

>NTDB_id=523648 JH273_RS16750 WP_274507695.1 3843600..3845333(+) (pilB) [Xanthomonas campestris strain 3054]
MNSVVTANLVGITGIARRLVQDGALDEGTARAAMDQAATAKVPLPQWFSEKKLVTASQLAAANSVEFGMPLLDVSSFDSN
QNAIKLVSEELLQKFQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLG
DDDDEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRISARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=523648 JH273_RS16750 WP_274507695.1 3843600..3845333(+) (pilB) [Xanthomonas campestris strain 3054]
ATGAATAGCGTCGTCACGGCTAACTTGGTTGGCATTACCGGAATTGCCCGGCGTCTTGTGCAGGACGGTGCTCTGGACGA
AGGCACGGCACGTGCTGCGATGGATCAGGCCGCTACTGCCAAGGTTCCATTGCCTCAATGGTTTTCAGAGAAGAAGCTGG
TGACCGCTTCGCAACTTGCGGCGGCCAACTCGGTGGAGTTCGGGATGCCGCTACTTGATGTGTCCTCGTTTGACTCTAAT
CAGAATGCAATCAAATTGGTTAGCGAAGAACTGCTGCAAAAGTTTCAAGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCAGGGCGCTGGACGATATCAAGTTCCACACCAATCTGGTGGTCGAGCCGA
TCCTGGTCGACGAAGACCAGATCCGTCGCACATTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGCTCCTCGCTCGGC
GACGACGATGATGAGATGGGGGATCTGGACGTCTCCACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAGCCAGCGCATCTCCGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCAACCCTGTTCGGCGAGA
AGGTGGTGTTGCGTATCCTGGACGCCAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCGCTCGGGATTCTCAACGACGAGACGCGCAATATTTCCACGGCTGAGGATCCGGTCGAAATCCGCTTGCCCG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCATATGGT
GCTGTCGACACTGCACACCAACGATGCGCCGCAAACCATTGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACATCA
CCTCCTCGGTGACGCTGGTCATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGA
GTGCACCGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGACGGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCTGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTAATGAAG
GCTTCGCACGGCGTGACCAGCCTGGCCGAAATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

98.44

0.548
  pilB Acinetobacter baylyi ADP1

55.282

98.44

0.544
  pilB Legionella pneumophila strain ERS1305867

51.761

98.44

0.51
  pilF Neisseria gonorrhoeae MS11

48.592

98.44

0.478
  pilB Vibrio cholerae strain A1552

49.365

95.494

0.471
  pilB Vibrio campbellii strain DS40M4

46.046

98.614

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

46.181

97.574

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

35.738

100

0.378