Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JH282_RS05350 Genome accession   NZ_CP066953
Coordinates   1265699..1267429 (+) Length   576 a.a.
NCBI ID   WP_057671826.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain 10103     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1260699..1272429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH282_RS05330 (JH282_05305) coaE 1262024..1262647 (-) 624 WP_011038217.1 dephospho-CoA kinase -
  JH282_RS05335 (JH282_05310) - 1262661..1263524 (-) 864 WP_057671659.1 A24 family peptidase -
  JH282_RS05340 (JH282_05315) pilC 1263531..1264793 (-) 1263 WP_057671824.1 type II secretion system F family protein Machinery gene
  JH282_RS05345 (JH282_05320) pilA 1265135..1265563 (+) 429 WP_057671660.1 pilin Machinery gene
  JH282_RS05350 (JH282_05325) pilB 1265699..1267429 (+) 1731 WP_057671826.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH282_RS05360 (JH282_05335) pilR 1267848..1269242 (-) 1395 WP_057671663.1 sigma-54 dependent transcriptional regulator Regulator
  JH282_RS05365 (JH282_05340) - 1269451..1271061 (-) 1611 WP_057671664.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62599.69 Da        Isoelectric Point: 5.6001

>NTDB_id=523535 JH282_RS05350 WP_057671826.1 1265699..1267429(+) (pilB) [Xanthomonas campestris pv. campestris strain 10103]
MSAVAANLVGITGIARRLVQDGALDEISARTAMDQAAAAKTPLPQWFSERKLVSASQLAAANAVEFGMPLMDVSVFDASQ
NAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLGS
DDDDIGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPV
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPE
HALLAEGFTPEQVANIDLYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMKA
SHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=523535 JH282_RS05350 WP_057671826.1 1265699..1267429(+) (pilB) [Xanthomonas campestris pv. campestris strain 10103]
ATGAGTGCAGTCGCGGCGAACTTAGTAGGAATTACAGGCATTGCACGCCGCCTAGTGCAAGACGGAGCGCTTGATGAAAT
AAGTGCCCGTACCGCCATGGACCAAGCCGCGGCAGCGAAGACTCCGCTTCCCCAATGGTTCTCAGAGAGAAAGCTGGTTT
CCGCGTCTCAACTGGCTGCAGCCAACGCAGTCGAGTTCGGCATGCCTCTCATGGACGTTTCCGTGTTCGATGCTAGTCAG
AACGCGGTCAAGCTGGTCAGTGAGGAGTTGCTCCAGAAGTATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTT
TGTGGGAGTGAGCAACCCGACCCAGACACGGGCGCTGGACGACATCAAGTTCCACACTAACTTGGTGGTCGAGCCGATTC
TGGTCGACGAGGACCAGATCCGTCGCACGCTAGAGCAGTGGCAGGCCAGTAACGCCGCGCTCGGCTCGTCGCTGGGGAGC
GACGATGACGATATAGGCGATCTAGACGTGTCCGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGC
CAAGGGCGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACATCC
ATTTCGAGCCCTATGAAGATGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCGGTG
AAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGACGG
GCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGATTTTCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAGAAGG
TGGTGTTGCGTATCCTGGACGCCAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCGGACCAGCAGAAGCTG
TTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCTGTA
TACCGCGCTCGGGATTCTCAACGACGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGCCCGGCG
TCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGCCACATGGTGCT
GTCGACGTTGCACACCAACGATGCGCCGCAGACCATTGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCACCT
CGTCGGTGACCCTGGTCATCGCGCAGCGCTTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCGGAA
CATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGACCTCTATGAAGCGGTCGGTTGCGATGAGTG
CACGGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGACCGACGAGATCGGTGCGATCGTGCTGGAAG
GCGGCAATGCGATGCAGATCGCCGAGGCCGCACAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAGGCT
TCGCACGGGGTGACCAGCCTGGCCGAAATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.338

98.611

0.556
  pilB Acinetobacter baylyi ADP1

55.282

98.611

0.545
  pilB Legionella pneumophila strain ERS1305867

52.083

100

0.521
  pilF Neisseria gonorrhoeae MS11

49.12

98.611

0.484
  pilB Vibrio cholerae strain A1552

49.817

94.792

0.472
  pilB Vibrio campbellii strain DS40M4

46.046

98.785

0.455
  pilB Vibrio parahaemolyticus RIMD 2210633

46.333

97.049

0.45
  pilB/pilB1 Synechocystis sp. PCC 6803

35.41

100

0.375