Detailed information NTG0523248

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: JH261_RS05595
• Genome accession: NZ_CP066926
• Coordinates: 1355705..1357438 (+)
• Length: 577 a.a.
• NCBI ID: WP_228441061.1
• Uniprot ID: -
• Organism: Xanthomonas campestris pv. incanae strain CFBP6690
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1348581..1365248	1355705..1357438	within	0

Gene organization within MGE regions

Location: 1348581..1365248

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JH261_RS05560 (JH261_05545)	pilC	1348581..1349843 (-)	1263	WP_274318282.1	type II secretion system F family protein	Machinery gene
JH261_RS05565 (JH261_05550)	-	1349953..1351920 (-)	1968	WP_274318283.1	hypothetical protein	-
JH261_RS05570 (JH261_05555)	-	1351976..1352392 (-)	417	WP_047124751.1	pilin	-
JH261_RS05575 (JH261_05560)	-	1352617..1353117 (+)	501	WP_274318284.1	EamA family transporter	-
JH261_RS05580 (JH261_05565)	-	1353122..1353916 (+)	795	WP_274318285.1	glycosyltransferase family 2 protein	-
JH261_RS05585 (JH261_05570)	-	1353913..1354944 (+)	1032	WP_274318286.1	glycosyltransferase family 2 protein	-
JH261_RS05590 (JH261_05575)	-	1354955..1355554 (+)	600	WP_274318287.1	methionine biosynthesis protein MetW	-
JH261_RS05595 (JH261_05580)	pilB	1355705..1357438 (+)	1734	WP_228441061.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JH261_RS05600 (JH261_05585)	-	1357621..1358358 (-)	738	WP_274318288.1	zeta toxin family protein	-
JH261_RS05605 (JH261_05590)	-	1358358..1358714 (-)	357	WP_139328384.1	hypothetical protein	-
JH261_RS05610 (JH261_05595)	pilR	1358912..1360306 (-)	1395	WP_274318289.1	sigma-54 dependent transcriptional regulator	Regulator
JH261_RS05615 (JH261_05600)	-	1360515..1362125 (-)	1611	WP_274318290.1	HAMP domain-containing sensor histidine kinase	-
JH261_RS05620 (JH261_05605)	sucC	1362359..1363528 (+)	1170	WP_011038209.1	ADP-forming succinate--CoA ligase subunit beta	-
JH261_RS05625 (JH261_05610)	sucD	1363553..1364428 (+)	876	WP_011038208.1	succinate--CoA ligase subunit alpha	-
JH261_RS05630 (JH261_05615)	-	1364532..1364942 (+)	411	WP_323475224.1	DNA-binding protein	-
JH261_RS05635 (JH261_05620)	-	1364946..1365248 (+)	303	WP_011038207.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Sequence

Protein

• Length: 577 a.a.
• Molecular weight: 62862.96 Da
• Isoelectric Point: 5.4486

>NTDB_id=523248 JH261_RS05595 WP_228441061.1 1355705..1357438(+) (pilB) [Xanthomonas campestris pv. incanae strain CFBP6690]
MNSVVTANLVGITGIARRLVQDGALDEGTARAAMDQAATAKVPLPQWFSEKKLVTASQLAAANSVEFGMPLLDVSSFDSN
QNAIKLVSEELLQKFQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSMG
DDDDEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide

• Length: 1734 bp

>NTDB_id=523248 JH261_RS05595 WP_228441061.1 1355705..1357438(+) (pilB) [Xanthomonas campestris pv. incanae strain CFBP6690]
ATGAATAGCGTCGTCACGGCTAACTTGGTTGGCATTACCGGAATTGCCCGGCGTCTTGTGCAGGACGGTGCTCTGGACGA
AGGCACGGCACGTGCTGCGATGGATCAGGCCGCTACTGCCAAGGTTCCATTGCCTCAATGGTTTTCAGAGAAGAAGCTGG
TGACCGCTTCGCAACTTGCGGCGGCCAACTCGGTGGAGTTCGGGATGCCGCTGCTTGATGTGTCCTCGTTCGACTCTAAT
CAGAATGCAATCAAATTGGTTAGCGAAGAACTGCTGCAAAAGTTTCAAGTGCTGCCCCTGTTCAAGCGCGGTAATCGGTT
GTTCGTAGGGGTGAGCAACCCGACCCAGACCCGGGCTCTGGACGACATCAAGTTCCATACAAACCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGTCGCACCTTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGCTCTTCGATGGGC
GACGACGATGATGAAATGGGGGATCTTGACGTCTCCACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAAGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGACTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCTTGAAGGTAATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTTCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGTTGCGTATCCTGGACGCCAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGTTACGAGGCGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCGCTCGGGATTCTCAATGACGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGCCCG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTTGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGCCACATGGT
GCTGTCGACACTGCACACCAACGATGCGCCGCAAACCATTGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACATCA
CCTCGTCGGTGACCCTGGTCATCGCGCAGCGCCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAGGCGGTCGGTTGCGATGA
ATGCACGGAAGGCTACAAGGGCCGCACCGGTATCTATCAGGTGATGCCGATGACGGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCTGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTAATGAAG
GCTTCGCACGGCGTGACCAGCCTGGCCGAAATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (201-468)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (50-153)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	55.81	98.44	0.549
pilB	Acinetobacter baylyi ADP1	55.634	98.44	0.548
pilB	Legionella pneumophila strain ERS1305867	51.585	98.44	0.508
pilF	Neisseria gonorrhoeae MS11	48.768	98.44	0.48
pilB	Vibrio cholerae strain A1552	49.365	95.494	0.471
pilB	Vibrio campbellii strain DS40M4	45.518	98.614	0.449
pilB	Vibrio parahaemolyticus RIMD 2210633	45.648	97.574	0.445
pilB/pilB1	Synechocystis sp. PCC 6803	35.738	100	0.378