Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JFJ84_RS01870 Genome accession   NZ_CP066739
Coordinates   369060..370451 (+) Length   463 a.a.
NCBI ID   WP_136122165.1    Uniprot ID   -
Organism   Histophilus somni strain ASc-MMNZ-VFA-069     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 364060..375451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFJ84_RS01855 (JFJ84_01850) clpX 365452..366696 (-) 1245 WP_012341085.1 ATP-dependent protease ATP-binding subunit ClpX -
  JFJ84_RS01860 (JFJ84_01855) clpP 366712..367293 (-) 582 WP_012341086.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  JFJ84_RS01865 (JFJ84_01860) tig 367379..368677 (-) 1299 WP_011609585.1 trigger factor -
  JFJ84_RS01870 (JFJ84_01865) pilB 369060..370451 (+) 1392 WP_136122165.1 GspE/PulE family protein Machinery gene
  JFJ84_RS01875 (JFJ84_01870) - 370485..371744 (-) 1260 WP_012341088.1 HlyC/CorC family transporter -
  JFJ84_RS01880 (JFJ84_01875) - 371814..372611 (-) 798 WP_011609582.1 inner membrane protein YpjD -
  JFJ84_RS01885 (JFJ84_01880) ffh 372761..374137 (+) 1377 WP_012341090.1 signal recognition particle protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52927.78 Da        Isoelectric Point: 6.2978

>NTDB_id=522200 JFJ84_RS01870 WP_136122165.1 369060..370451(+) (pilB) [Histophilus somni strain ASc-MMNZ-VFA-069]
MSPIVMSIDGDTYEITPHLWQRNQQQSQILLRYFAIPLQENEQTLWLGVDSLNNISACETFAFLYGKIVEPVLLNNQLLK
QLLQNLSPQQNNMFVEEQPINQYVAEHRIEVTEKSDEPVIQLLDHIFENALKQHVSDIHIEPQMNCLQIRFRIDGILQCQ
SPLPLSLSKRILSRLKLLAKLDISETRLPQDGRFHFKTTFSDILDFRLSTLPTNMGEKAVLRLQQNKPVQLSFAELGMTE
NQQKSFKQALSQPQGLILVTGPTGSGKSISLYTALQWLNDKHKHIMTAEDPIEIELNGIIQCQINPQIGLDFSRLLRTFL
RQDPDIIMLGEIRDNESAIMALRAAQTGHLVLSTLHTNDAPSAVSRLLQLGVKQHEIDNSLLLVIAQRLVRKKCPHTENE
NCTCHQKYQGRIGVYQFLQPHLIDNHICYQTDYAHLRESAMEKVRLEITDLAEVDRVIGQSSE

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=522200 JFJ84_RS01870 WP_136122165.1 369060..370451(+) (pilB) [Histophilus somni strain ASc-MMNZ-VFA-069]
ATGTCTCCTATAGTGATGTCGATCGATGGAGATACTTATGAAATAACACCGCACTTATGGCAAAGAAATCAGCAACAATC
CCAAATTCTGCTACGCTATTTTGCCATTCCTTTACAGGAAAATGAACAAACGTTATGGTTAGGCGTTGATAGTCTAAATA
ATATTAGTGCCTGTGAAACATTTGCATTTTTGTATGGAAAAATTGTAGAACCGGTTTTACTTAATAACCAATTACTTAAA
CAGTTACTACAAAACTTATCACCACAGCAAAATAATATGTTCGTTGAAGAGCAACCTATAAATCAATATGTAGCCGAGCA
TCGTATAGAAGTAACTGAAAAGTCTGATGAACCCGTGATTCAACTTTTAGATCACATTTTTGAAAATGCGTTAAAGCAAC
ATGTATCAGATATTCATATTGAACCGCAAATGAATTGCTTGCAAATACGTTTCAGAATAGACGGTATTTTACAATGCCAA
TCGCCTCTTCCGCTTTCGCTGAGTAAGCGTATTCTTTCACGTTTAAAGTTACTGGCCAAATTGGATATAAGCGAAACACG
GTTACCTCAAGATGGACGATTTCATTTTAAAACAACATTTTCTGACATCCTTGATTTTCGTTTATCAACCCTTCCGACGA
ATATGGGTGAAAAAGCCGTGTTGCGTTTACAGCAAAATAAACCTGTACAGTTGAGTTTTGCGGAACTCGGCATGACAGAA
AACCAGCAAAAATCATTCAAACAGGCACTATCTCAACCCCAAGGACTCATTTTAGTGACCGGTCCAACAGGGAGTGGAAA
AAGTATCTCACTTTACACCGCACTTCAATGGCTGAATGATAAGCACAAACATATTATGACAGCGGAAGATCCGATAGAAA
TTGAATTGAACGGTATCATTCAATGTCAAATAAATCCGCAGATCGGGTTAGATTTTAGCCGACTGTTAAGAACTTTTCTT
CGTCAAGATCCTGACATTATTATGTTGGGTGAAATTCGGGATAATGAAAGTGCAATAATGGCATTAAGAGCAGCACAAAC
CGGACATCTTGTTCTCTCAACTTTACATACAAATGATGCTCCGTCGGCAGTTTCACGCTTGCTTCAATTAGGGGTAAAGC
AACATGAAATTGACAATAGTTTATTATTGGTCATCGCTCAACGCTTGGTACGAAAAAAATGTCCACATACAGAAAATGAA
AATTGCACTTGTCATCAGAAATATCAAGGGCGAATTGGTGTTTATCAATTTTTACAACCGCATCTAATAGATAATCATAT
TTGCTACCAAACGGATTATGCTCATTTGCGTGAAAGTGCAATGGAAAAAGTGCGGTTAGAAATAACAGATTTAGCAGAAG
TGGATAGAGTTATTGGACAAAGTAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(126-392)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-90)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.33

99.568

0.68
  pilB Haemophilus influenzae Rd KW20

67.609

99.352

0.672
  pilB Glaesserella parasuis strain SC1401

57.333

97.192

0.557
  pilB Vibrio campbellii strain DS40M4

40.456

100

0.421
  pilB Vibrio cholerae strain A1552

39.212

100

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

38.969

100

0.408
  pilB Legionella pneumophila strain ERS1305867

37.045

100

0.395
  pilB Acinetobacter baylyi ADP1

36.128

100

0.391
  pilB Acinetobacter baumannii D1279779

37.474

100

0.391
  pilF Neisseria gonorrhoeae MS11

35.865

100

0.367