Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JFL60_RS01900 Genome accession   NZ_CP066567
Coordinates   366509..367900 (+) Length   463 a.a.
NCBI ID   WP_101812545.1    Uniprot ID   A0AAX2RXF4
Organism   Histophilus somni strain ASc-MMNZ-VFA-079     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 366178..408267 366509..367900 within 0


Gene organization within MGE regions


Location: 366178..408267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFL60_RS01900 (JFL60_01900) pilB 366509..367900 (+) 1392 WP_101812545.1 GspE/PulE family protein Machinery gene
  JFL60_RS01905 (JFL60_01905) - 367934..369193 (-) 1260 WP_132994681.1 HlyC/CorC family transporter -
  JFL60_RS01910 (JFL60_01910) - 369263..370060 (-) 798 WP_132994680.1 cytochrome C assembly family protein -
  JFL60_RS01915 (JFL60_01915) ffh 370208..371584 (+) 1377 WP_132994679.1 signal recognition particle protein -
  JFL60_RS01920 (JFL60_01920) dauA 371627..373366 (-) 1740 WP_075293625.1 C4-dicarboxylic acid transporter DauA -
  JFL60_RS01930 (JFL60_01930) - 373823..375064 (+) 1242 WP_075293624.1 tyrosine-type recombinase/integrase -
  JFL60_RS01935 (JFL60_01935) - 375026..375211 (-) 186 WP_011609578.1 helix-turn-helix transcriptional regulator -
  JFL60_RS01940 (JFL60_01940) - 375224..376018 (-) 795 WP_075293623.1 hypothetical protein -
  JFL60_RS01945 (JFL60_01945) - 376050..377237 (-) 1188 WP_075293622.1 DNA cytosine methyltransferase -
  JFL60_RS01950 (JFL60_01950) - 377266..377994 (-) 729 WP_075293621.1 hypothetical protein -
  JFL60_RS01955 (JFL60_01955) - 378000..379040 (-) 1041 WP_198986221.1 hypothetical protein -
  JFL60_RS01960 (JFL60_01960) - 379090..379758 (-) 669 WP_075293619.1 translocation protein TolB precursor -
  JFL60_RS01965 (JFL60_01965) - 379751..380687 (-) 937 Protein_378 recombinase RecT -
  JFL60_RS01970 (JFL60_01970) - 380699..380998 (-) 300 WP_011609572.1 hypothetical protein -
  JFL60_RS01975 (JFL60_01975) - 381048..381332 (-) 285 WP_075293616.1 hypothetical protein -
  JFL60_RS01980 (JFL60_01980) - 381496..381918 (-) 423 WP_198973831.1 hypothetical protein -
  JFL60_RS01985 (JFL60_01985) - 381915..382199 (-) 285 WP_075293616.1 hypothetical protein -
  JFL60_RS01990 (JFL60_01990) - 382284..382640 (-) 357 Protein_383 Bro-N domain-containing protein -
  JFL60_RS01995 (JFL60_01995) - 383004..383492 (-) 489 WP_132994672.1 hypothetical protein -
  JFL60_RS02000 (JFL60_02000) - 383748..383936 (+) 189 WP_075290735.1 hypothetical protein -
  JFL60_RS02005 (JFL60_02005) - 383923..384099 (-) 177 WP_157666559.1 hypothetical protein -
  JFL60_RS02010 (JFL60_02010) - 384299..384565 (-) 267 WP_011609564.1 hypothetical protein -
  JFL60_RS02015 (JFL60_02015) - 384818..385036 (+) 219 WP_198986222.1 helix-turn-helix domain-containing protein -
  JFL60_RS02020 (JFL60_02020) - 385033..385857 (+) 825 WP_012341695.1 hypothetical protein -
  JFL60_RS02025 (JFL60_02025) - 385854..386591 (+) 738 WP_233121828.1 replication protein P -
  JFL60_RS02030 (JFL60_02030) - 386519..386956 (+) 438 Protein_391 DNA N-6-adenine-methyltransferase -
  JFL60_RS09710 - 386969..387294 (+) 326 Protein_392 recombination protein NinG -
  JFL60_RS02035 (JFL60_02035) - 387284..387688 (+) 405 WP_132994671.1 hypothetical protein -
  JFL60_RS02040 (JFL60_02040) - 387705..388214 (+) 510 Protein_394 hypothetical protein -
  JFL60_RS02045 (JFL60_02045) - 388325..388561 (+) 237 WP_012341704.1 hypothetical protein -
  JFL60_RS02050 (JFL60_02050) - 388554..389090 (+) 537 WP_011609542.1 lysozyme -
  JFL60_RS02055 (JFL60_02055) - 389063..389392 (+) 330 WP_132994669.1 DUF2570 family protein -
  JFL60_RS02060 (JFL60_02060) - 389346..389564 (+) 219 WP_041605057.1 hypothetical protein -
  JFL60_RS02065 (JFL60_02065) - 389589..390284 (+) 696 WP_075294025.1 terminase -
  JFL60_RS02070 (JFL60_02070) - 390284..391831 (+) 1548 WP_132994668.1 TerL -
  JFL60_RS02075 (JFL60_02075) - 391833..394010 (+) 2178 WP_132994667.1 hypothetical protein -
  JFL60_RS02080 (JFL60_02080) - 394073..394267 (+) 195 WP_132994664.1 hypothetical protein -
  JFL60_RS02085 (JFL60_02085) - 394260..395840 (+) 1581 WP_198986223.1 hypothetical protein -
  JFL60_RS02090 (JFL60_02090) - 395818..396048 (+) 231 WP_198986322.1 hypothetical protein -
  JFL60_RS10060 (JFL60_02095) - 396062..396178 (+) 117 WP_416182597.1 hypothetical protein -
  JFL60_RS02100 (JFL60_02100) - 396284..399958 (+) 3675 WP_198986225.1 LPD38 domain-containing protein -
  JFL60_RS02105 (JFL60_02105) - 400311..400613 (+) 303 WP_011609534.1 XRE family transcriptional regulator -
  JFL60_RS02110 (JFL60_02110) - 400711..400893 (+) 183 WP_011609533.1 hypothetical protein -
  JFL60_RS02115 (JFL60_02115) - 400971..401834 (+) 864 WP_011609532.1 hypothetical protein -
  JFL60_RS02120 (JFL60_02120) - 401860..402855 (+) 996 WP_132994663.1 N4-gp56 family major capsid protein -
  JFL60_RS02125 (JFL60_02125) - 402917..403363 (+) 447 WP_011609530.1 SAP domain-containing protein -
  JFL60_RS02130 (JFL60_02130) - 403360..403749 (+) 390 WP_132994662.1 hypothetical protein -
  JFL60_RS09945 - 403808..403942 (+) 135 WP_262363290.1 hypothetical protein -
  JFL60_RS02135 (JFL60_02135) - 404034..406013 (+) 1980 WP_132994661.1 hypothetical protein -
  JFL60_RS09715 - 406006..406896 (+) 891 WP_233121833.1 hypothetical protein -
  JFL60_RS09720 - 406893..407375 (+) 483 WP_233121835.1 phage tail protein -
  JFL60_RS09725 - 407441..407617 (+) 177 WP_233121837.1 hypothetical protein -
  JFL60_RS02145 (JFL60_02145) - 407653..408267 (+) 615 WP_132995793.1 DUF4376 domain-containing protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52954.80 Da        Isoelectric Point: 6.2978

>NTDB_id=521030 JFL60_RS01900 WP_101812545.1 366509..367900(+) (pilB) [Histophilus somni strain ASc-MMNZ-VFA-079]
MSPIVMSLDGDTYEITPHLWQRNQQQSQILLRYFAIPLQENEQTLWLGVDSLNNISACETFAFLYGKIVEPVLLNNQLLK
QLLQNLSPQQNNMFVEEQPINQYVAEHRIEVTEKSDEPVIQLLDHIFENALKQHVSDIHIEPQMNCLQIRFRIDGILQCQ
SPLPLSLSKRILSRLKLLAKLDISETRLPQDGRFHFKTTFSDILDFRLSTLPTNMGEKAVLRLQQNKPVQLSFAELGMTE
NQQKSFKQALSQPQGLILVTGPTGSGKSISLYTALQWLNDKHKHIMTAEDPIEIELNGIIQCQINPQIGLDFSRLLRTFL
RQDPDIIMLGEIRDNESAIMALRAAQTGHLVLSTLHTNDAPSAVSRLLQLGVKQHEIDNSLLLVIAQRLVRKKCPHTENE
NCTCHQKYQGRIGVYQFLQPHLIDNHICYQTDYAHLRESAMEKVRLEITDLAEVDRVIGQSNE

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=521030 JFL60_RS01900 WP_101812545.1 366509..367900(+) (pilB) [Histophilus somni strain ASc-MMNZ-VFA-079]
ATGTCTCCGATAGTGATGTCGCTCGATGGAGATACTTATGAAATAACACCGCACTTATGGCAAAGAAATCAGCAACAATC
CCAAATTCTGCTACGCTATTTTGCCATTCCTTTACAGGAAAATGAACAAACGTTATGGTTAGGCGTTGATAGTCTAAATA
ATATTAGTGCCTGTGAAACATTTGCATTTTTGTATGGAAAAATTGTAGAACCGGTTTTACTTAATAACCAATTACTTAAA
CAGTTACTACAAAACTTATCACCACAGCAAAATAATATGTTCGTTGAAGAGCAACCTATAAATCAATATGTAGCCGAGCA
TCGTATAGAAGTAACTGAAAAGTCTGATGAACCCGTGATTCAACTTTTAGATCACATTTTTGAAAATGCGTTAAAGCAAC
ATGTATCAGATATTCATATTGAACCGCAAATGAATTGCTTGCAAATACGTTTCAGAATAGACGGTATTTTACAATGCCAA
TCGCCTCTTCCGCTTTCGCTGAGTAAGCGTATTCTTTCACGTTTAAAGTTACTGGCCAAATTGGATATAAGCGAAACACG
GTTACCTCAAGATGGACGATTTCATTTTAAAACAACATTTTCTGACATCCTTGATTTTCGTTTATCAACCCTTCCGACGA
ATATGGGTGAAAAAGCCGTGTTGCGTTTACAGCAAAATAAACCTGTACAGTTGAGTTTTGCGGAACTCGGCATGACAGAA
AACCAGCAAAAATCATTCAAACAGGCACTATCTCAACCTCAAGGACTCATTTTAGTGACAGGCCCAACAGGGAGTGGAAA
AAGTATCTCACTTTACACCGCACTTCAATGGCTGAATGATAAGCACAAACATATTATGACAGCGGAAGATCCGATAGAAA
TTGAATTGAACGGTATCATTCAATGTCAAATAAATCCGCAGATCGGGTTAGATTTTAGCCGGCTGTTAAGAACTTTTCTT
CGTCAAGATCCTGACATTATTATGTTGGGTGAAATTCGGGATAACGAAAGTGCAATAATGGCATTAAGAGCAGCACAAAC
TGGACATCTTGTTCTCTCAACTTTACATACAAATGATGCTCCGTCGGCAGTCTCACGCTTGCTTCAATTAGGGGTAAAGC
AACATGAAATTGACAATAGTTTATTATTGGTCATCGCTCAACGTTTGGTACGAAAAAAATGCCCACATACAGAAAATGAA
AATTGCACTTGTCATCAGAAATATCAAGGGCGAATTGGTGTTTATCAATTTTTACAACCGCATCTAATAGATAATCATAT
TTGCTACCAAACGGATTATGCTCATTTGCGTGAAAGTGCAATGGAAAAAGTGCGGTTAGAAATAACAGATTTAGCAGAAG
TGGATAGAGTTATTGGACAAAGTAATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-90)

Type II/IV secretion system protein (T2SSE)

(126-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.113

99.568

0.678
  pilB Haemophilus influenzae Rd KW20

67.609

99.352

0.672
  pilB Glaesserella parasuis strain SC1401

57.333

97.192

0.557
  pilB Vibrio campbellii strain DS40M4

40.456

100

0.421
  pilB Vibrio cholerae strain A1552

39.212

100

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

38.969

100

0.408
  pilB Legionella pneumophila strain ERS1305867

37.045

100

0.395
  pilB Acinetobacter baylyi ADP1

36.128

100

0.391
  pilB Acinetobacter baumannii D1279779

37.474

100

0.391
  pilF Neisseria gonorrhoeae MS11

35.865

100

0.367