Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   I9H09_RS22340 Genome accession   NZ_CP066270
Coordinates   4960890..4962995 (-) Length   701 a.a.
NCBI ID   WP_005888212.1    Uniprot ID   -
Organism   Pseudomonas tremae strain PA-1-10F     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4955890..4967995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H09_RS22325 (I9H09_22240) - 4957512..4959137 (-) 1626 WP_122347002.1 AAA family ATPase -
  I9H09_RS22330 (I9H09_22245) aroB 4959148..4960251 (-) 1104 WP_122347003.1 3-dehydroquinate synthase -
  I9H09_RS22335 (I9H09_22250) aroK 4960367..4960885 (-) 519 WP_005888214.1 shikimate kinase AroK -
  I9H09_RS22340 (I9H09_22255) pilQ 4960890..4962995 (-) 2106 WP_005888212.1 type IV pilus secretin PilQ Machinery gene
  I9H09_RS22345 (I9H09_22260) pilP 4963043..4963570 (-) 528 WP_005888210.1 type 4a pilus biogenesis lipoprotein PilP -
  I9H09_RS22350 (I9H09_22265) pilO 4963567..4964190 (-) 624 WP_005888208.1 type 4a pilus biogenesis protein PilO -
  I9H09_RS22355 (I9H09_22270) - 4964187..4964762 (-) 576 WP_005888206.1 PilN domain-containing protein -
  I9H09_RS22360 (I9H09_22275) pilM 4964762..4965826 (-) 1065 WP_005888204.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75454.89 Da        Isoelectric Point: 5.1826

>NTDB_id=519070 I9H09_RS22340 WP_005888212.1 4960890..4962995(-) (pilQ) [Pseudomonas tremae strain PA-1-10F]
MTRIFSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLSFDAPVPAPRGYTTAQPARIALDLPGVTSQLSTKSRE
LGTGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGASAAQASQPTTAMGAQRVAVPPPTPASARPYVPA
GAKAIRNIDFQRGELGEGNVVIDLSNASIAPDIQEQGGKIRVDFAKTLLPEPLRVRLDVKDFATPVQFVSASTSGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDVDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAEAKADERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGRTNLSGSS
KWSAGGLDNNGDEAGNTGNNLGPNIPFVDLGAANATTGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=519070 I9H09_RS22340 WP_005888212.1 4960890..4962995(-) (pilQ) [Pseudomonas tremae strain PA-1-10F]
ATGACCCGGATTTTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTTTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTGGCTGCCCTGCCGGGCGATCGCATCGAGCTCAAGCTTTCATTCGATGCGCCCGTGCCTGCGCCTCGTG
GCTACACTACCGCGCAACCGGCTCGCATCGCGCTGGACCTGCCGGGCGTGACCAGCCAGTTATCGACCAAAAGTCGAGAG
CTGGGCACCGGCAATGCACGCAGTGTGGTCGTGGTGCAGGCCCAGGATCGCACGCGGGTGATCATCAACCTGACGACGCT
GTCGCCTTACAGCTCGCGGGTCGACGGCAACAACCTGTTCGTGGTCATCGGGCAAGGAGCATCTGCCGCGCAGGCCTCTC
AGCCAACCACCGCAATGGGCGCGCAGAGAGTGGCTGTGCCGCCGCCCACTCCGGCTTCCGCCAGGCCTTACGTGCCTGCC
GGCGCCAAGGCAATCAGAAATATCGACTTCCAGCGCGGCGAGTTGGGTGAAGGTAATGTGGTCATCGACCTGAGCAATGC
CAGCATTGCCCCTGATATTCAGGAGCAGGGCGGCAAGATCCGCGTCGATTTCGCCAAGACCCTGCTGCCTGAACCGCTGC
GTGTCCGCCTCGACGTCAAGGACTTCGCGACCCCCGTGCAGTTCGTCAGTGCTTCTACTTCTGGCGACAAGGCCAGCATC
CTGATCGAGCCGTCGGGCGCTTTCGATTACTCGGCGTTCCAGACCGAAAACAAGCTGACGATCAGTGTGCGTCCGTTGAC
CAACGAGGACGTGGACCGCCGCGCCGCCGACAAGCCGGTGTACACCGGTGAGAAGCTGTCGCTGAACTTCCAAGACATCG
ATGTGCGTTCGGTGCTGCAACTGATTGCCGACTTCACCAACCTTAATCTGGTCGCCAGCGACACTGTCCAGGGTGGCATC
ACGCTGCGCTTGCAGAACGTGCCGTGGGATCAGGCACTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGAT
CGGCAACGTCCTGCTGGTCGCGCCAGCTGACGAAATCGCCGCCCGCGAACGTCAGGAGCTGGAATCGCTCAAGCAGATCG
CCGAACTGGCACCGCTGCGTCGTGAACTGCTGCAGGTCAACTACGCCAAGGCCGCCGATATCGCCAAGCTGTTCCAGTCG
GTGACCAGCGCCGAGGCCAAGGCTGACGAGCGTGGCTCCATTACAGTGGACGACCGCACCAATAACATCATTGCCTATCA
GACCCAGGACCGCCTGGACGAACTTCGCCGTATCGTTTCGCAACTGGACATTCCGGTGCGTCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAACAGTTGGGTGTGCGTTGGGGCGGCCGGACCAACCTGTCGGGTTCCAGC
AAATGGAGCGCTGGCGGTCTGGACAATAACGGCGATGAGGCAGGCAATACCGGCAACAATCTGGGGCCCAACATTCCGTT
TGTCGACCTGGGCGCTGCTAATGCGACGACAGGTATCGGCCTGGGTTTCGTGACCAACAACACGCTGCTGGACCTTGAGC
TGAGCGCGATGGAAAAAACCGGCAACGGTGAAATCGTCTCCCAGCCCAAAGTGGTCACGTCCGACAAGGAAACTGCCAAG
ATCCTCAAGGGCACGGAAATCCCGTACCAGGAATCCAGCTCCAGCGGCGCAACCACCGTGAGCTTCAAGGAGGCGTCCCT
GTCGCTTGAAGTGACGCCGCAGATCACGCCCGATAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGGTGCTTGGCGTTCCGCCTATCAAGAAAAACGAAGTCAACGCCAAAGTGCTGATTTCCGACGGCGAAACC
ATCGTCATTGGCGGCGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTCGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.299

100

0.745
  comQ Acinetobacter baylyi ADP1

41.088

100

0.431
  pilQ Acinetobacter baumannii strain A118

38.926

100

0.414
  pilQ Acinetobacter baumannii D1279779

38.926

100

0.414
  pilQ Legionella pneumophila strain ERS1305867

38.316

98.288

0.377
  pilQ Legionella pneumophila strain Lp02

38.171

98.288

0.375