Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I9H06_RS03855 Genome accession   NZ_CP066267
Coordinates   809628..811322 (-) Length   564 a.a.
NCBI ID   WP_236534609.1    Uniprot ID   -
Organism   Pseudomonas tremae strain PA-1-12B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 804628..816322
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H06_RS03825 (I9H06_03820) - 805405..805938 (-) 534 WP_053481610.1 MOSC domain-containing protein -
  I9H06_RS03830 (I9H06_03825) - 805935..806564 (-) 630 WP_005895647.1 DUF1780 domain-containing protein -
  I9H06_RS03835 (I9H06_03830) yacG 806708..806917 (-) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  I9H06_RS03840 (I9H06_03835) coaE 806914..807537 (-) 624 WP_122347409.1 dephospho-CoA kinase -
  I9H06_RS03845 (I9H06_03840) pilD 807534..808406 (-) 873 WP_122347408.1 A24 family peptidase Machinery gene
  I9H06_RS03850 (I9H06_03845) pilC 808408..809625 (-) 1218 WP_024690386.1 type II secretion system F family protein Machinery gene
  I9H06_RS03855 (I9H06_03850) pilB 809628..811322 (-) 1695 WP_236534609.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I9H06_RS03860 (I9H06_03855) - 811549..811953 (+) 405 WP_005895657.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I9H06_RS03865 (I9H06_03860) - 812496..813083 (+) 588 WP_055002518.1 hypothetical protein -
  I9H06_RS03870 (I9H06_03865) - 813080..813487 (+) 408 WP_053481442.1 hypothetical protein -
  I9H06_RS03875 (I9H06_03870) - 813531..814076 (-) 546 WP_054998406.1 DUF1523 family protein -
  I9H06_RS03880 (I9H06_03875) - 814196..815194 (+) 999 WP_054998407.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62478.67 Da        Isoelectric Point: 6.1206

>NTDB_id=519025 I9H06_RS03855 WP_236534609.1 809628..811322(-) (pilB) [Pseudomonas tremae strain PA-1-12B]
MTDAVLTGLAKQLVLAELLTETVAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLIRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDNDSGLGNLEDVDLGLD
IEPADGKETSLAAQNDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKEIDIPRETLIQEGFPESK
IGTFKIFGPEGCEHCNGGYRGRVGIYEVVKKTAELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=519025 I9H06_RS03855 WP_236534609.1 809628..811322(-) (pilB) [Pseudomonas tremae strain PA-1-12B]
ATGACTGATGCTGTACTCACTGGCTTGGCCAAACAATTGGTCCTTGCCGAGCTGCTTACCGAAACCGTTGCGCAGCAAGC
GTATCAACAAGCGCGCCGCGACAAAATTTCGTTGGTGAGCTATCTGGTTCAGAACAAGCTGGTCAAAAGCCTGACGCTCG
CAGAGATGGCTTCGGATCAGTTTGGTGTCCCTTTTATGGATCTGGCCAGCCTGGACAAGGAAAGCCAGCCTAAGGGGCTG
GTGAGTGAAAAGCTTATTCGACAGCATCATGCCCTTCCACTATGGCGAAGAGGTAACAAGCTGTTTATCGGTATTTCGGA
CCCGACTAACCATCAGGCGGTGACCGATATTCAGTTCAGCACCGGCCTGAACACTGAAGCGATTCTGGTCGAAGACGACA
AGCTGACCACTGCGATAGACCGTTTTTTTGACAATGACAGCGGGCTGGGCAATCTGGAAGATGTCGATCTGGGTCTGGAT
ATCGAACCCGCTGATGGCAAGGAAACCTCCCTTGCTGCACAGAATGATGCTGACGACGCCCCGGTGGTGCGCTTCGTCAA
CAAAATGCTGTTGGATGCTATCAGGCTAGGATCATCAGACCTGCACTTCGAGCCCTATGAAAAGATCTTTCGTGTGCGCT
TGCGTACCGACGGCATTCTGCACGAAGTCGCCAGGCCGCCGATTCATCTGGCAAACCGTATTGCTGCCCGCTTGAAAGTC
ATGGCCAGCCTCGATATTTCCGAGCGACGTAAACCGCAGGATGGCCGGGTCAAGTTAAGGGTGTCCAAAACAAAGGCCAT
CGATTTCCGTATGAACACCTTGCCCACGCTATGGGGCGAGAAGATCGTGATGCGTATTCTTGATCCGACCAGTGCGCAGA
TGGGCATTGACGCTCTGGGCTATGAACCAGAGCAAAAGGCACTGTATCTGGAGGCACTAAAGCAGCCACAAGGCATGATT
CTTGTGACCGGCCCTACGGGTTCTGGCAAAACTGTTTCCCTGTATACCGGGCTGAATATCCTCAATACTGTGGACATCAA
CATTTCCACCGCTGAAGACCCGGTAGAGATCAACCTTGAAGGCATCAATCAGGTCAACGTCAACCCACGCCAAGGACTCG
ACTTTTCTCAGGCATTGCGCGCCTTTCTGCGTCAAGATCCCGACGTAATCATGGTTGGTGAGATTCGCGACCTGGAGACT
GCAGAAATCGCCATCAAGGCCTCACAGACAGGCCATATGGTGCTTTCAACGCTGCACACCAACAGCGCAGCAGAAACCCT
GACGCGTTTACATCACATGGGCGTCGCTGCATTCAACATTGCCACAGCGATCAACCTGATTATCGCGCAACGACTTGCAC
GCAAACTTTGCAGCCACTGCAAGAAAGAAATCGATATTCCGCGGGAGACGTTGATTCAGGAAGGCTTCCCGGAATCAAAA
ATCGGAACGTTCAAAATCTTTGGCCCCGAGGGCTGCGAACATTGCAACGGCGGCTATAGAGGCCGGGTAGGTATTTACGA
AGTGGTCAAAAAAACAGCAGAGCTGGAACGCATTATCATGGAAGAAGGGAACTCGCTTGAGATATCCAGACAGATGCGCA
AAGACGGTTTCAATGATCTGCGCACTTCGGGCTTGTCAAAAGCGATGCAAGGCATCACCAGCCTTGAAGAAGTCAACCGC
GTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.774

100

0.58
  pilB Acinetobacter baylyi ADP1

56.89

100

0.571
  pilB Legionella pneumophila strain ERS1305867

55.752

100

0.559
  pilB Vibrio cholerae strain A1552

51.423

99.645

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

51.071

99.291

0.507
  pilB Vibrio campbellii strain DS40M4

49.647

100

0.498
  pilF Neisseria gonorrhoeae MS11

47.687

99.645

0.475
  pilF Thermus thermophilus HB27

38.516

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.783

93.262

0.362