Detailed information NTG0519023

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: I9H06_RS03845
• Genome accession: NZ_CP066267
• Coordinates: 807534..808406 (-)
• Length: 290 a.a.
• NCBI ID: WP_122347408.1
• Uniprot ID: -
• Organism: Pseudomonas tremae strain PA-1-12B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 802534..813406

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I9H06_RS03820 (I9H06_03815)	-	803889..805187 (-)	1299	WP_005895640.1	NAD(P)/FAD-dependent oxidoreductase	-
I9H06_RS03825 (I9H06_03820)	-	805405..805938 (-)	534	WP_053481610.1	MOSC domain-containing protein	-
I9H06_RS03830 (I9H06_03825)	-	805935..806564 (-)	630	WP_005895647.1	DUF1780 domain-containing protein	-
I9H06_RS03835 (I9H06_03830)	yacG	806708..806917 (-)	210	WP_003381207.1	DNA gyrase inhibitor YacG	-
I9H06_RS03840 (I9H06_03835)	coaE	806914..807537 (-)	624	WP_122347409.1	dephospho-CoA kinase	-
I9H06_RS03845 (I9H06_03840)	pilD	807534..808406 (-)	873	WP_122347408.1	A24 family peptidase	Machinery gene
I9H06_RS03850 (I9H06_03845)	pilC	808408..809625 (-)	1218	WP_024690386.1	type II secretion system F family protein	Machinery gene
I9H06_RS03855 (I9H06_03850)	pilB	809628..811322 (-)	1695	WP_236534609.1	type IV-A pilus assembly ATPase PilB	Machinery gene
I9H06_RS03860 (I9H06_03855)	-	811549..811953 (+)	405	WP_005895657.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
I9H06_RS03865 (I9H06_03860)	-	812496..813083 (+)	588	WP_055002518.1	hypothetical protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32058.23 Da
• Isoelectric Point: 8.3734

>NTDB_id=519023 I9H06_RS03845 WP_122347408.1 807534..808406(-) (pilD) [Pseudomonas tremae strain PA-1-12B]
MPLLDLLASSPLAFVTTCCILGLIIGSFLNVVVYRLPIMMERDWKAQSREMLGLPAEQDQPVFNLNRPRSSCPHCAHKIR
PWENLPILSYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTVLLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=519023 I9H06_RS03845 WP_122347408.1 807534..808406(-) (pilD) [Pseudomonas tremae strain PA-1-12B]
ATGCCCCTCCTCGACCTCCTGGCCAGCTCCCCCTTGGCATTCGTCACCACCTGCTGCATTCTCGGCTTGATCATAGGAAG
CTTCCTGAACGTAGTCGTCTACCGCTTGCCAATAATGATGGAGCGCGACTGGAAAGCACAATCCCGTGAAATGCTCGGCC
TCCCCGCCGAACAGGACCAGCCCGTTTTCAACCTGAACCGACCCCGTTCCAGCTGCCCGCACTGCGCGCACAAGATACGC
CCGTGGGAAAACCTGCCGATCCTCAGCTACCTGTTGCTGCGTGGGAAATGCTCGCAATGCAAAGCGCCGATCAGCAAACG
CTACCCGTTGGTCGAGCTGACCTGCGCAGTTTTGTCGGCCTATGTCGCCTGGCATTTTGGCTTTGGCTGGCAGGCTGCAG
CCATGTTGGTGTTGAGCTGGGGATTGCTGGCGATGAGTCTGATCGATGCCGATCATCAGCTGTTGCCTGATTCTCTGGTG
CTGCCATTGTTGTGGTTGGGCCTGATTGTTAACGCCTTTGGGCTGTTCACATCGCTAAATGATGCGCTCTGGGGGGCTGT
GGCGGGATATCTGGCGCTGTGGTCGGTGTTTTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGATT
TCAAACTGCTGGCCATGCTTGGCGCGTGGGGCGGTTGGCAGATTCTGCCGCTGACCGTGCTGCTGTCGTCGCTGGTGGGC
GCAGTGCTTGGGGTGATCATGATGCGCGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTTGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.035	98.276	0.531
pilD	Vibrio campbellii strain DS40M4	50.877	98.276	0.5
pilD	Acinetobacter baumannii D1279779	51.636	94.828	0.49
pilD	Acinetobacter nosocomialis M2	51.273	94.828	0.486
pilD	Neisseria gonorrhoeae MS11	51.493	92.414	0.476