Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JDU55_RS06940 Genome accession   NZ_CP066223
Coordinates   1443834..1444268 (-) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain PM-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1438834..1449268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JDU55_RS06910 - 1439401..1439670 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  JDU55_RS06915 yacG 1439670..1439873 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  JDU55_RS06920 coaE 1439884..1440504 (-) 621 WP_005751078.1 dephospho-CoA kinase -
  JDU55_RS06925 - 1440519..1441241 (-) 723 WP_005727302.1 prepilin peptidase -
  JDU55_RS06930 - 1441241..1442452 (-) 1212 WP_005751077.1 type II secretion system F family protein -
  JDU55_RS06935 pilB 1442452..1443834 (-) 1383 WP_005751075.1 GspE/PulE family protein Machinery gene
  JDU55_RS06940 pilA 1443834..1444268 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  JDU55_RS06945 ampD 1444428..1444994 (+) 567 WP_005751074.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JDU55_RS06950 rppH 1445580..1446176 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  JDU55_RS06955 - 1446179..1446973 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  JDU55_RS06960 lgt 1446983..1447786 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  JDU55_RS06965 - 1447792..1448643 (+) 852 WP_005751072.1 thymidylate synthase -
  JDU55_RS06970 tadA 1448658..1449158 (+) 501 WP_005751071.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=518554 JDU55_RS06940 WP_005741096.1 1443834..1444268(-) (pilA) [Pasteurella multocida strain PM-1]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=518554 JDU55_RS06940 WP_005741096.1 1443834..1444268(-) (pilA) [Pasteurella multocida strain PM-1]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCTGCTCAAGGTGTTTCTTGGACAGTGAATTGTGGTGCTC
AATCTGACATTTTTCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368