Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KZY09_RS20275 Genome accession   NZ_CP080172
Coordinates   4164028..4164495 (+) Length   155 a.a.
NCBI ID   WP_000340171.1    Uniprot ID   -
Organism   Escherichia coli strain PK8566     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4159028..4169495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZY09_RS20230 (KZY09_20205) rplP 4159138..4159548 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  KZY09_RS20235 (KZY09_20210) rpsC 4159561..4160262 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  KZY09_RS20240 (KZY09_20215) rplV 4160280..4160612 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  KZY09_RS20245 (KZY09_20220) rpsS 4160627..4160905 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  KZY09_RS20250 (KZY09_20225) rplB 4160922..4161743 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  KZY09_RS20255 (KZY09_20230) rplW 4161761..4162063 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  KZY09_RS20260 (KZY09_20235) rplD 4162060..4162665 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  KZY09_RS20265 (KZY09_20240) rplC 4162676..4163305 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  KZY09_RS20270 (KZY09_20245) rpsJ 4163338..4163649 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  KZY09_RS20275 (KZY09_20250) pilD 4164028..4164495 (+) 468 WP_000340171.1 prepilin peptidase Machinery gene
  KZY09_RS20280 (KZY09_20255) bfr 4164492..4164968 (-) 477 WP_000675515.1 bacterioferritin -
  KZY09_RS20285 (KZY09_20260) bfd 4165041..4165235 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KZY09_RS20290 (KZY09_20265) tuf 4165418..4166602 (-) 1185 WP_000031783.1 elongation factor Tu -
  KZY09_RS20295 (KZY09_20270) fusA 4166673..4168787 (-) 2115 WP_000124700.1 elongation factor G -
  KZY09_RS20300 (KZY09_20275) rpsG 4168884..4169354 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16938.08 Da        Isoelectric Point: 8.1296

>NTDB_id=518047 KZY09_RS20275 WP_000340171.1 4164028..4164495(+) (pilD) [Escherichia coli strain PK8566]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=518047 KZY09_RS20275 WP_000340171.1 4164028..4164495(+) (pilD) [Escherichia coli strain PK8566]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361
  pilD Neisseria gonorrhoeae MS11

38.095

94.839

0.361