Detailed information NTG0517583

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: JC624_RS14510
• Genome accession: NZ_CP066124
• Coordinates: 3034764..3035882 (+)
• Length: 372 a.a.
• NCBI ID: WP_005070855.1
• Uniprot ID: A0A013SVN8
• Organism: Acinetobacter pittii strain 32292
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3029764..3040882

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JC624_RS14500	yggS	3032888..3033580 (-)	693	WP_068563798.1	YggS family pyridoxal phosphate-dependent enzyme	-
JC624_RS14505	pilT	3033707..3034744 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
JC624_RS14510	pilU	3034764..3035882 (+)	1119	WP_005070855.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
JC624_RS14515	fur	3035952..3036389 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
JC624_RS14520	omlA	3036501..3036899 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
JC624_RS14525	-	3036964..3037278 (-)	315	WP_068563794.1	RnfH family protein	-
JC624_RS14530	-	3037278..3038345 (-)	1068	WP_139112248.1	hypothetical protein	-
JC624_RS14535	-	3038499..3038954 (-)	456	WP_002121698.1	bacteriohemerythrin	-
JC624_RS14540	-	3039206..3040054 (-)	849	WP_014205980.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41931.15 Da
• Isoelectric Point: 6.6404

>NTDB_id=517583 JC624_RS14510 WP_005070855.1 3034764..3035882(+) (pilU) [Acinetobacter pittii strain 32292]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=517583 JC624_RS14510 WP_005070855.1 3034764..3035882(+) (pilU) [Acinetobacter pittii strain 32292]
ATGGATTTTAATGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGACAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAACAGCGTGACATGCCCGGCATGGTACTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTGCTTAAAGATTTATCGATGACTAAACGCGGCATCATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTCGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCCGATGTGATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACAGGTCACCTTGTATTAGCAACGATGCATGCAAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGACTTATCGCTAAACTTAAAGGCCATGATTGCACA
ACAGCTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCCCCATTAATTTCAGACT
ATATTCGTAAAGGTGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTCGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTTGACCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A013SVN8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.733	99.731	0.685
pilU	Vibrio cholerae strain A1552	53.784	99.462	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363