Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   KW2_RS11265 Genome accession   NC_022369
Coordinates   2339782..2340783 (-) Length   333 a.a.
NCBI ID   WP_014573419.1    Uniprot ID   -
Organism   Lactococcus cremoris subsp. cremoris KW2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2334782..2345783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW2_RS11225 (kw2_2261) - 2334853..2335494 (-) 642 WP_021037994.1 phosphotransferase -
  KW2_RS11230 (kw2_2262) - 2335526..2336014 (-) 489 WP_011677247.1 GAF domain-containing protein -
  KW2_RS11235 (kw2_2263) - 2336098..2336370 (+) 273 WP_011677248.1 chorismate mutase -
  KW2_RS11240 (kw2_2264) - 2336404..2336853 (-) 450 WP_021037995.1 hypothetical protein -
  KW2_RS11245 (kw2_2265) - 2337071..2337271 (+) 201 WP_011836112.1 hypothetical protein -
  KW2_RS11250 (kw2_2266) glnA 2337332..2338672 (-) 1341 WP_021037996.1 type I glutamate--ammonia ligase -
  KW2_RS11255 (kw2_2267) - 2338734..2339099 (-) 366 WP_011836114.1 MerR family transcriptional regulator -
  KW2_RS11260 (kw2_2268) - 2339200..2339637 (-) 438 WP_021037997.1 low molecular weight protein-tyrosine-phosphatase -
  KW2_RS11265 (kw2_2269) ruvB 2339782..2340783 (-) 1002 WP_014573419.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  KW2_RS11270 (kw2_2270) ruvA 2340930..2341523 (-) 594 WP_021037998.1 Holliday junction branch migration protein RuvA Machinery gene
  KW2_RS11275 (kw2_2271) hexB 2341575..2343545 (-) 1971 WP_021037999.1 DNA mismatch repair endonuclease MutL Machinery gene
  KW2_RS11280 (kw2_2272) - 2343675..2344370 (-) 696 WP_021038000.1 hypothetical protein -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 37679.26 Da        Isoelectric Point: 4.5859

>NTDB_id=51699 KW2_RS11265 WP_014573419.1 2339782..2340783(-) (ruvB) [Lactococcus cremoris subsp. cremoris KW2]
MNDILNKEPMEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMAFVIANELGVNI
KQTAGPAIEKPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMEDFYIDIMLGSGDGSRSVHLDLPPFTLVGATTR
AGMLSNPLRARFGISSHMEYYQERDLEEIVKRTADIFEVEVIDNAAMEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVD
KAITDKALTILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLMRTKQGRKVTQR
AYEHLGYVYNEEE

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=51699 KW2_RS11265 WP_014573419.1 2339782..2340783(-) (ruvB) [Lactococcus cremoris subsp. cremoris KW2]
ATGAATGATATATTAAATAAAGAGCCAATGGAAGAGGATTATGGCATTGAAAAATCTCTTCGCCCGCAATTTTTCAATCA
ATACATTGGTCAAGACAAAGTCAAAGAACAGTTAGAAATCTTTATTAAAGCGGCAAAAATGCGGGAAGAAGTTTTGGACC
ATGTTTTACTCTTTGGTCCTCCCGGGCTTGGTAAAACAACCATGGCATTTGTGATTGCCAATGAACTTGGGGTTAATATT
AAACAAACCGCTGGTCCTGCTATTGAGAAACCAGGTGATTTAGTCGCAATTTTGAATGAATTAGAACCTGGGGATGTCCT
ATTTATTGATGAAATTCATCGGATGCCCATGCAGGTTGAAGAAGTGCTTTATAGCGCCATGGAAGACTTCTACATTGATA
TCATGCTTGGTTCTGGTGACGGCTCACGTTCGGTCCATTTAGACTTACCACCTTTTACTTTGGTTGGCGCAACAACGAGA
GCTGGGATGCTTTCTAATCCATTGCGGGCCCGCTTTGGTATTTCTAGTCACATGGAATATTATCAAGAGCGTGATTTAGA
GGAAATTGTTAAAAGAACAGCTGATATTTTTGAGGTTGAAGTCATTGATAATGCTGCTATGGAAATTGCTTTACGCAGTC
GTGGGACTCCGCGGATTGCCAATCGTTTACTTAAACGAGTGCGTGATTTTGCCCAAATCATGGGTGATGGACGAGTGGAT
AAAGCTATTACAGACAAAGCCTTGACAATTCTTGATGTTGATGCTGCTGGTTTGGATTATATTGACCAAAAAATATTGCG
CACAATGATTGAAATGTATCACGGTGGACCTGTTGGAATTGGGACTTTGGCAGTAAATATTGCTGAGGACCGCGAAACTG
TCGAAGACATGTATGAGCCCTATTTGATTCAAAAAGGATTTTTGATGAGAACTAAACAGGGACGTAAAGTTACCCAAAGA
GCTTATGAGCATTTGGGTTACGTTTATAATGAAGAGGAATGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-325)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(20-178)

RuvB AAA lid domain (AAA_lid_4)

(181-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Streptococcus pneumoniae R6

78.916

99.7

0.787
  ruvB Streptococcus pneumoniae D39

78.916

99.7

0.787
  ruvB Streptococcus pneumoniae TIGR4

78.614

99.7

0.784
  ruvB Bacillus subtilis subsp. subtilis str. 168

61.18

96.697

0.592
  ruvB Helicobacter pylori 26695

51.242

96.697

0.495
  ruvB Synechocystis sp. PCC 6803

51.613

93.093

0.48

Multiple sequence alignment