Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6H44_RS06220 Genome accession   NZ_CP065983
Coordinates   1259714..1261123 (-) Length   469 a.a.
NCBI ID   WP_006719140.1    Uniprot ID   E6KZA7
Organism   Aggregatibacter segnis strain FDAARGOS_987     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1254714..1266123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H44_RS06190 (I6H44_06190) - 1254907..1256283 (-) 1377 WP_006719150.1 sodium:alanine symporter family protein -
  I6H44_RS06195 (I6H44_06195) - 1256662..1256934 (-) 273 WP_006719149.1 GNAT family N-acetyltransferase -
  I6H44_RS06200 (I6H44_06200) yacG 1256931..1257146 (-) 216 WP_006719148.1 DNA gyrase inhibitor YacG -
  I6H44_RS06205 (I6H44_06205) coaE 1257136..1257759 (-) 624 WP_006719146.1 dephospho-CoA kinase -
  I6H44_RS06210 (I6H44_06210) - 1257809..1258501 (-) 693 WP_006719144.1 A24 family peptidase -
  I6H44_RS06215 (I6H44_06215) pilC 1258498..1259721 (-) 1224 WP_006719142.1 type II secretion system F family protein Machinery gene
  I6H44_RS06220 (I6H44_06220) pilB 1259714..1261123 (-) 1410 WP_006719140.1 GspE/PulE family protein Machinery gene
  I6H44_RS06225 (I6H44_06225) pilA 1261152..1261604 (-) 453 WP_006719138.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  I6H44_RS06230 (I6H44_06230) ampD 1261731..1262285 (+) 555 WP_006719137.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I6H44_RS06235 (I6H44_06235) - 1262873..1263418 (+) 546 WP_006719135.1 carboxymuconolactone decarboxylase family protein -
  I6H44_RS06240 (I6H44_06240) - 1263582..1264376 (+) 795 WP_006719133.1 formate/nitrite transporter family protein -
  I6H44_RS06245 (I6H44_06245) nanQ 1264473..1264943 (-) 471 WP_006719132.1 N-acetylneuraminate anomerase -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 53311.83 Da        Isoelectric Point: 6.1159

>NTDB_id=515939 I6H44_RS06220 WP_006719140.1 1259714..1261123(-) (pilB) [Aggregatibacter segnis strain FDAARGOS_987]
MQHLMERHYSVINQQGEEFCITPELWLRNQQQQTALLRYLALPLKEDVQTLWLGLDSLTNLAACETFSFLSGKHIEPVLL
PSHVLKSALQDLTKSEKVEENQPLFYSTELQEQPDTQQIDEPIIQQLNQIFENATQKKASDIHLEPQADGLQVRFRIDGV
LQVQEQISRSLANRLISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLATQFGEKAVLRLQQNRPVQLEFSEL
GMTESQQQLFRHALSQPQGLILVTGPTGSGKSISLYTALQYLNTPEKHIMTAEDPIEIQLPGIIQTQVNNPIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDAPSAISRLQQLGIQRHEIDSSLLLVIAQRLVRKRCLK
CGGNPNHFCDCHQGYKGRVGVYQFLQPQLHHPQGYEMDFDDLRQSALEKIKNGITDLAEIQRVLGQPHD

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=515939 I6H44_RS06220 WP_006719140.1 1259714..1261123(-) (pilB) [Aggregatibacter segnis strain FDAARGOS_987]
ATGCAACATCTGATGGAACGGCATTATTCGGTCATCAATCAACAGGGCGAAGAATTTTGTATTACGCCTGAGCTTTGGCT
TAGAAATCAGCAACAACAAACCGCCCTCTTACGTTATTTGGCGTTACCGTTAAAAGAAGATGTCCAAACGCTGTGGCTAG
GATTGGATTCGTTAACCAATCTTGCCGCTTGTGAAACCTTTTCCTTTCTCAGTGGAAAGCACATTGAGCCTGTGTTATTG
CCTAGCCATGTACTCAAAAGCGCACTACAGGATTTAACAAAATCTGAAAAAGTCGAGGAAAATCAACCGCTCTTTTATTC
AACTGAACTACAAGAACAGCCTGATACCCAGCAAATTGATGAGCCGATCATTCAACAACTCAATCAGATTTTTGAAAACG
CTACACAGAAAAAAGCATCCGATATTCATTTGGAACCGCAAGCCGACGGATTACAAGTGCGTTTTCGTATTGATGGCGTA
TTGCAAGTACAAGAACAAATCTCACGTTCTTTGGCAAACCGCTTAATTTCCCGTCTGAAATTATTGGCGAAATTGGATAT
TAGCGAGACACGTTTACCACAAGATGGCCGCTTTCAGTTTAAAACCACCTTTTCTGATATTTTAGATTTTCGCCTTTCGA
CCCTTGCGACCCAATTTGGCGAAAAAGCCGTGTTGCGTTTACAGCAAAATCGCCCCGTTCAGTTGGAATTCAGTGAATTG
GGCATGACCGAATCGCAGCAACAACTTTTCCGTCATGCATTAAGCCAACCCCAAGGATTGATTTTAGTAACCGGACCGAC
CGGTAGCGGCAAAAGTATTTCTTTGTATACGGCATTACAGTATTTAAATACACCGGAAAAACACATCATGACGGCAGAGG
ATCCCATTGAAATTCAGTTGCCAGGCATTATTCAAACTCAAGTAAATAATCCTATTGGCTTGGATTTCAGCCGTCTCCTT
CGCACCTTCTTACGCCAAGATCCCGACATCATTATGCTAGGTGAAATTCGTGATGAAGAAAGTGCTGCCATGGCACTACG
CGCCGCACAAACAGGACACTTAGTCCTTTCTACCTTACACACCAATGATGCTCCTTCCGCCATTTCCCGCTTGCAACAGT
TGGGTATTCAGCGCCATGAAATCGACAGCAGTTTGCTGCTCGTTATTGCGCAACGATTGGTGCGTAAACGTTGTCTAAAG
TGCGGTGGAAATCCCAACCATTTTTGCGACTGTCATCAAGGTTACAAAGGGCGTGTCGGGGTATATCAATTCCTACAACC
TCAACTACACCATCCGCAAGGCTATGAAATGGATTTCGATGATTTACGTCAAAGCGCCTTAGAAAAAATCAAAAACGGCA
TAACTGATTTGGCTGAAATTCAGCGAGTATTAGGGCAGCCCCATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-396)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(33-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E6KZA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

72.588

97.228

0.706
  pilB Haemophilus influenzae Rd KW20

72.149

97.228

0.701
  pilB Glaesserella parasuis strain SC1401

56.344

99.147

0.559
  pilB Vibrio parahaemolyticus RIMD 2210633

42.259

100

0.431
  pilB Vibrio campbellii strain DS40M4

42.259

100

0.431
  pilB Vibrio cholerae strain A1552

41.545

100

0.424
  pilB Acinetobacter baylyi ADP1

38.956

100

0.414
  pilB Legionella pneumophila strain ERS1305867

38.677

100

0.412
  pilB Acinetobacter baumannii D1279779

39.669

100

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.632

100

0.38