Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   I6H44_RS06225 Genome accession   NZ_CP065983
Coordinates   1261152..1261604 (-) Length   150 a.a.
NCBI ID   WP_006719138.1    Uniprot ID   E6KZA6
Organism   Aggregatibacter segnis strain FDAARGOS_987     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1256152..1266604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H44_RS06195 (I6H44_06195) - 1256662..1256934 (-) 273 WP_006719149.1 GNAT family N-acetyltransferase -
  I6H44_RS06200 (I6H44_06200) yacG 1256931..1257146 (-) 216 WP_006719148.1 DNA gyrase inhibitor YacG -
  I6H44_RS06205 (I6H44_06205) coaE 1257136..1257759 (-) 624 WP_006719146.1 dephospho-CoA kinase -
  I6H44_RS06210 (I6H44_06210) - 1257809..1258501 (-) 693 WP_006719144.1 A24 family peptidase -
  I6H44_RS06215 (I6H44_06215) pilC 1258498..1259721 (-) 1224 WP_006719142.1 type II secretion system F family protein Machinery gene
  I6H44_RS06220 (I6H44_06220) pilB 1259714..1261123 (-) 1410 WP_006719140.1 GspE/PulE family protein Machinery gene
  I6H44_RS06225 (I6H44_06225) pilA 1261152..1261604 (-) 453 WP_006719138.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  I6H44_RS06230 (I6H44_06230) ampD 1261731..1262285 (+) 555 WP_006719137.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I6H44_RS06235 (I6H44_06235) - 1262873..1263418 (+) 546 WP_006719135.1 carboxymuconolactone decarboxylase family protein -
  I6H44_RS06240 (I6H44_06240) - 1263582..1264376 (+) 795 WP_006719133.1 formate/nitrite transporter family protein -
  I6H44_RS06245 (I6H44_06245) nanQ 1264473..1264943 (-) 471 WP_006719132.1 N-acetylneuraminate anomerase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15595.91 Da        Isoelectric Point: 8.4701

>NTDB_id=515940 I6H44_RS06225 WP_006719138.1 1261152..1261604(-) (pilA) [Aggregatibacter segnis strain FDAARGOS_987]
MTIQSVFISLKSVKKGFTLIELMIVIAIVAILATVAVPSYQNYTKKAAISELLQASAPLRAEVELCIYNTGKTDNCSGGQ
NGIQADVTDATKQKYVKSTAVKSGAITVTGKSSLENISYTLSASGTAATGVSWTASCGNNAEIFPAGFCS

Nucleotide


Download         Length: 453 bp        

>NTDB_id=515940 I6H44_RS06225 WP_006719138.1 1261152..1261604(-) (pilA) [Aggregatibacter segnis strain FDAARGOS_987]
ATGACAATTCAATCCGTTTTTATCTCTCTGAAATCCGTTAAAAAAGGGTTTACATTAATCGAATTGATGATTGTGATTGC
GATCGTCGCAATTCTCGCAACCGTCGCCGTGCCATCTTACCAAAATTACACGAAAAAAGCGGCTATTTCTGAGTTGTTAC
AGGCTTCTGCGCCGCTTCGTGCCGAAGTGGAACTGTGTATTTATAACACCGGCAAAACCGACAACTGCAGCGGAGGACAA
AATGGCATTCAAGCCGATGTTACCGATGCCACAAAACAAAAATACGTAAAATCCACGGCGGTAAAAAGTGGCGCTATCAC
TGTAACCGGGAAAAGCAGTCTCGAAAATATTAGCTACACCCTCAGCGCCTCCGGCACCGCTGCCACGGGGGTAAGCTGGA
CGGCTTCCTGCGGTAATAATGCAGAGATTTTCCCAGCTGGCTTTTGCTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E6KZA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

65

93.333

0.607
  pilA Haemophilus influenzae Rd KW20

61.111

96

0.587
  pilA Glaesserella parasuis strain SC1401

51.748

95.333

0.493