Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JC965_RS04190 Genome accession   NZ_CP065937
Coordinates   854573..856279 (-) Length   568 a.a.
NCBI ID   WP_198497742.1    Uniprot ID   A0A7T4C3W1
Organism   Aeromonas caviae strain Aero21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 849573..861279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC965_RS04160 (JC965_04160) mutT 850191..850595 (+) 405 WP_175429552.1 8-oxo-dGTP diphosphatase MutT -
  JC965_RS04165 (JC965_04165) yacG 850653..850847 (-) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  JC965_RS04170 (JC965_04170) zapD 850858..851580 (-) 723 WP_010675825.1 cell division protein ZapD -
  JC965_RS04175 (JC965_04175) coaE 851618..852232 (-) 615 WP_069785551.1 dephospho-CoA kinase -
  JC965_RS04180 (JC965_04180) pilD 852251..853126 (-) 876 WP_069785552.1 A24 family peptidase Machinery gene
  JC965_RS04185 (JC965_04185) pilC 853204..854445 (-) 1242 WP_010675822.1 type II secretion system F family protein Machinery gene
  JC965_RS04190 (JC965_04190) pilB 854573..856279 (-) 1707 WP_198497742.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  JC965_RS27825 (JC965_04195) - 856279..856668 (-) 390 WP_080989820.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  JC965_RS04200 (JC965_04200) nadC 856981..857844 (-) 864 WP_069785553.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JC965_RS04205 (JC965_04205) - 857848..858318 (-) 471 WP_042015101.1 retropepsin-like aspartic protease -
  JC965_RS04210 (JC965_04210) ampD 858447..859019 (+) 573 WP_039040507.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JC965_RS04215 (JC965_04215) pdhR 859296..860060 (+) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62677.06 Da        Isoelectric Point: 5.9708

>NTDB_id=515448 JC965_RS04190 WP_198497742.1 854573..856279(-) (pilB) [Aeromonas caviae strain Aero21]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIYQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNIFNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=515448 JC965_RS04190 WP_198497742.1 854573..856279(-) (pilB) [Aeromonas caviae strain Aero21]
ATGACCTCCTCACTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCCTCCCTCCTCAGTGAGCCGGACTCACAGCGTTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAGAACGATATCCTCGACAGCAAGGCGCTGG
CGGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTGGCGGCCTTCGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCTCTGGAGGATTTTGGCTTCAGTTTTGGCCTGCACACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGC
CTCAAGGTGATGGCGCGGCTCGATATTGCCGAACGGCGCCTGCCACAGGATGGCCGGATCAAACTCAAGATCTCACGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGTTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCTATCAACTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTGACCGGCCCGACGGGGTCAGGCAAGACGGTCTCACTCTATACCGGCCTCAACATCTTCAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTCGAGATAAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATACGGGAT
TTGGAAACCGCCGAGATCGCTATCAAGGCCGCCCAGACCGGTCATCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCAGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAAAAAGAAGGGATGCGCACCCTGCGTACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATAAACAGGATCACTACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T4C3W1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

54.69

99.472

0.544
  pilB Acinetobacter baumannii D1279779

54.497

99.824

0.544
  pilB Acinetobacter baylyi ADP1

54.529

99.12

0.54
  pilB Vibrio cholerae strain A1552

53.065

100

0.533
  pilB Vibrio campbellii strain DS40M4

50.177

99.648

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

49.556

99.12

0.491
  pilF Neisseria gonorrhoeae MS11

46.208

99.824

0.461
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

37.882

98.063

0.371