Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   I6G34_RS08205 Genome accession   NZ_CP065720
Coordinates   1767326..1769443 (+) Length   705 a.a.
NCBI ID   WP_102839587.1    Uniprot ID   -
Organism   Stutzerimonas frequens strain FDAARGOS_877     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1762326..1774443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G34_RS08185 (I6G34_08185) comM 1764492..1765556 (+) 1065 WP_102839584.1 pilus assembly protein PilM Machinery gene
  I6G34_RS08190 (I6G34_08190) - 1765556..1766128 (+) 573 WP_063539613.1 PilN domain-containing protein -
  I6G34_RS08195 (I6G34_08195) pilO 1766125..1766748 (+) 624 WP_102839585.1 type 4a pilus biogenesis protein PilO -
  I6G34_RS08200 (I6G34_08200) pilP 1766745..1767272 (+) 528 WP_102839586.1 type 4a pilus biogenesis lipoprotein PilP -
  I6G34_RS08205 (I6G34_08205) pilQ 1767326..1769443 (+) 2118 WP_102839587.1 type IV pilus secretin PilQ Machinery gene
  I6G34_RS08210 (I6G34_08210) aroK 1769449..1769994 (+) 546 WP_102839588.1 shikimate kinase AroK -
  I6G34_RS08215 (I6G34_08215) aroB 1770017..1771120 (+) 1104 WP_102839589.1 3-dehydroquinate synthase -
  I6G34_RS08220 (I6G34_08220) - 1771131..1772696 (+) 1566 WP_102839590.1 AAA family ATPase -

Sequence

Protein


Download         Length: 705 a.a.        Molecular weight: 75938.02 Da        Isoelectric Point: 4.7809

>NTDB_id=513460 I6G34_RS08205 WP_102839587.1 1767326..1769443(+) (pilQ) [Stutzerimonas frequens strain FDAARGOS_877]
MNSTLSRLGISVLVAFVSPAVLAANLNALDVAALPGDRVELKLAFDEPVAPPRGYTLEQPARIALDLPGVSNNLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLTSLVPYSTRVDGKNLFVVLGEKAGVSGPAAVSAQPSAVAQPSVTTAPAKAVAVGKA
IDNIDFRRGDDGAGNVIITLSDPSISSTIEEQGGKIRVNFAKAQLPESLRVRLDVQDFATPVRFVDSASQAGGASIAIEP
TGRYDYLAYQTDNKLTISIKPLTEDEAEKRKADRFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVSGNITLRL
QNVPWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTDN
GGMAEERGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNLWSGDKWNVWG
KDGDLGVEDGEEGQVGRFPGIRETPLNTPFVDMGAVGATSGFGLGFVTNNAILDLQLSAMEKTGNGEVVSQPKVVTADKE
TAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAATTGVPAIRKNEVNAKVLVT
DGETIVIGGVFSNTQSKSVDKVPFLGDLPFLGRLFRRDLVQDRKSELLVFITPRIMNNQAIQASR

Nucleotide


Download         Length: 2118 bp        

>NTDB_id=513460 I6G34_RS08205 WP_102839587.1 1767326..1769443(+) (pilQ) [Stutzerimonas frequens strain FDAARGOS_877]
ATGAATAGTACTCTCTCGCGCCTCGGCATTTCAGTGCTTGTGGCGTTCGTCTCGCCGGCTGTACTGGCAGCCAATCTCAA
TGCCTTGGATGTGGCCGCTCTGCCGGGCGATCGAGTCGAGCTCAAGCTGGCTTTCGACGAGCCCGTTGCGCCGCCGCGTG
GTTACACCCTTGAACAGCCTGCTCGCATTGCCCTGGATCTGCCGGGTGTATCGAACAATCTGGGAGCGAAGAATCGTGAG
TTGGGGGTTGGTAATGCACGTAGCGTTACGGTCGTTGAGGCGCAAGGACGTACTCGGTTGATTGTAAACCTCACTTCGCT
GGTGCCCTATTCCACTCGAGTGGATGGCAAGAATCTGTTCGTCGTGCTGGGCGAGAAGGCCGGCGTGAGTGGCCCGGCAG
CGGTTTCAGCGCAGCCGAGCGCAGTGGCGCAGCCATCTGTTACGACGGCTCCCGCGAAGGCGGTGGCTGTTGGTAAGGCC
ATTGACAACATTGACTTTCGGCGTGGCGATGATGGAGCCGGTAATGTGATTATTACGCTATCGGATCCATCAATCAGCTC
GACCATTGAGGAGCAGGGCGGAAAAATTCGCGTAAATTTCGCCAAGGCTCAGTTGCCGGAATCGCTGCGTGTGCGCCTGG
ATGTCCAAGACTTCGCTACGCCTGTCAGGTTCGTTGATTCCGCTTCTCAGGCAGGTGGTGCGAGCATCGCAATCGAACCG
ACCGGGCGATATGACTATTTGGCCTATCAGACCGACAACAAGCTGACTATCAGCATAAAACCTCTTACGGAAGACGAAGC
GGAAAAGCGTAAGGCTGATCGATTTGCTTATTCCGGGGAGAAGCTGTCGCTTAATTTCCAGGACATCGACGTTCGCTCGG
TATTGCAGCTAATCGCTGATTTTACCGATCTGAATCTGGTTGCAAGTGATACCGTTAGCGGCAATATCACGCTGCGTCTG
CAGAACGTGCCGTGGGATCAGGCTCTGGACCTGGTGCTCAAGACCAAGGGATTGGACAAGCGCCAGGTCGGTAACGTGCT
GCTGGTGGCTCCTGCCGACGAAATTGCCGCACGTGAACGTCAGGAGCTTGAGTCTCAGCGGCAGATTGCCGAACTGGCTC
CGCTTCGCCGTGAGGTGGTGCAGGTTAACTATGCCAAGGCCGCTGACATCGCCAGGCTATTTCAGTCGGTAACGGATAAT
GGCGGGATGGCGGAAGAGCGGGGGTCTATTGCGGTTGATGACCGCACCAACAATATCATTGCCTACCAGACTCAGGAGCG
TCTCGATGAGTTGCGTCGTATCGTGGCTCAACTGGATATTCCGGTTCGGCAGGTAATGATTGAGGCGCGCATAGTTGAGG
CAAACGTCGACTACGATAAGAGCCTTGGTGTTCGTTGGGGCGGCAACTTATGGTCGGGAGATAAATGGAACGTTTGGGGT
AAAGACGGCGATCTTGGCGTCGAGGATGGAGAAGAAGGACAGGTCGGGAGGTTTCCCGGCATTCGGGAAACCCCGCTTAA
CACCCCTTTCGTCGATATGGGCGCAGTGGGAGCAACGTCTGGATTTGGCTTAGGCTTCGTTACGAATAATGCAATTTTAG
ATCTACAGCTGTCTGCCATGGAGAAAACCGGTAATGGCGAGGTTGTCTCGCAGCCTAAGGTAGTCACCGCAGACAAAGAA
ACTGCAAAGATCCTCAAGGGCTCCGAAGTTCCTTATCAGGAGGCGAGCTCCAGTGGTGCAACGAGCACGTCGTTCAAGGA
GGCAGCTCTCTCGCTGGAAGTGACTCCGCAGATCACACCGGATAATCGCATCATCATGGAGGTCAAGGTCACTAAGGATG
AGCCAGACTTCTCTCAGGCAGCGACTACCGGAGTACCGGCCATTCGCAAGAATGAGGTTAACGCAAAGGTTCTGGTAACT
GATGGTGAGACGATCGTAATCGGTGGCGTTTTCTCCAATACGCAGAGCAAGTCAGTGGACAAGGTGCCGTTCCTAGGGGA
TCTCCCGTTCTTGGGTCGCCTGTTCCGCCGTGACCTAGTTCAGGACCGAAAGTCTGAACTGCTTGTGTTCATCACGCCGA
GGATCATGAATAATCAGGCGATTCAAGCCAGTCGCTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(305-351)

AMIN domain (AMIN)

(38-123)

Bacterial type II/III secretion system short domain (Secretin_N)

(378-444)

Bacterial type II and III secretion system protein (Secretin)

(539-696)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.853

100

0.753
  comQ Acinetobacter baylyi ADP1

41.16

100

0.413
  pilQ Acinetobacter baumannii strain A118

38.472

100

0.407
  pilQ Acinetobacter baumannii D1279779

38.472

100

0.407
  pilQ Legionella pneumophila strain Lp02

37.135

97.021

0.36