NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	I6G44_RS05495	Genome accession	NZ_CP065702
Coordinates	1112826..1113473 (-)	Length	215 a.a.
NCBI ID	WP_010906131.1	Uniprot ID	A0A3N6KQH1
Organism	Lactococcus lactis strain FDAARGOS_887
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1107826..1118473

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G44_RS05470 (I6G44_05470)	atpF	1107896..1108402 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
I6G44_RS05475 (I6G44_05475)	atpB	1108417..1109130 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
I6G44_RS05480 (I6G44_05480)	-	1109175..1109390 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
I6G44_RS05485 (I6G44_05485)	-	1109574..1110350 (-)	777	WP_004255244.1	alpha/beta hydrolase	-
I6G44_RS05490 (I6G44_05490)	comEC	1110635..1112845 (-)	2211	WP_010906130.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
I6G44_RS05495 (I6G44_05495)	comEA	1112826..1113473 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
I6G44_RS05500 (I6G44_05500)	-	1113533..1114894 (-)	1362	WP_021214597.1	ABC transporter permease	-
I6G44_RS05505 (I6G44_05505)	-	1114891..1115823 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
I6G44_RS05510 (I6G44_05510)	-	1115928..1116326 (-)	399	WP_004255236.1	hypothetical protein	-
I6G44_RS05515 (I6G44_05515)	-	1116430..1116993 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
I6G44_RS05520 (I6G44_05520)	-	1117166..1118344 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

Download Length: 215 a.a. Molecular weight: 23624.76 Da Isoelectric Point: 4.8659

                                >NTDB_id=513138 I6G44_RS05495 WP_010906131.1 1112826..1113473(-) (comEA) [Lactococcus lactis strain FDAARGOS_887]

MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP

NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD

LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

Download Length: 648 bp

                                >NTDB_id=513138 I6G44_RS05495 WP_010906131.1 1112826..1113473(-) (comEA) [Lactococcus lactis strain FDAARGOS_887]

ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT

TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT

CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT

AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA

GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG

AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT

TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA

TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT

TTGAATAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(70-125)

Helix-hairpin-helix motif (HHH_3)

(151-212)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A3N6KQH1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386

Detailed information NTG0513138