Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   I6G71_RS00150 Genome accession   NZ_CP065653
Coordinates   23609..25285 (+) Length   558 a.a.
NCBI ID   WP_082308679.1    Uniprot ID   -
Organism   Neisseria meningitidis strain FDAARGOS_914     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 18609..30285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G71_RS00105 (I6G71_00105) rpmB 18699..18932 (+) 234 WP_002216391.1 50S ribosomal protein L28 -
  I6G71_RS00110 (I6G71_00110) rpmG 18964..19119 (+) 156 WP_002212306.1 50S ribosomal protein L33 -
  I6G71_RS00115 (I6G71_00115) ubiM 19390..20574 (-) 1185 WP_002239495.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  I6G71_RS11595 - 20738..20860 (-) 123 Protein_21 IS5/IS1182 family transposase -
  I6G71_RS00120 (I6G71_00120) rpmA 20913..21185 (-) 273 WP_002212328.1 50S ribosomal protein L27 -
  I6G71_RS00125 (I6G71_00125) rplU 21210..21518 (-) 309 WP_002216394.1 50S ribosomal protein L21 -
  I6G71_RS00135 (I6G71_00135) ispB 21742..22716 (+) 975 WP_002216396.1 octaprenyl diphosphate synthase -
  I6G71_RS00140 (I6G71_00140) - 22721..23167 (+) 447 WP_002233503.1 DUF441 domain-containing protein -
  I6G71_RS00150 (I6G71_00150) pilF 23609..25285 (+) 1677 WP_082308679.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6G71_RS00155 (I6G71_00155) - 25370..25579 (-) 210 WP_002249247.1 DNA gyrase inhibitor YacG -
  I6G71_RS00160 (I6G71_00160) coaE 25572..26204 (-) 633 WP_002226983.1 dephospho-CoA kinase -
  I6G71_RS00165 (I6G71_00165) pilD 26206..27066 (-) 861 WP_002226036.1 prepilin peptidase Machinery gene
  I6G71_RS00170 (I6G71_00170) pilG 27140..28372 (-) 1233 WP_002248468.1 type 4 pilus assembly protein PilG Machinery gene

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61993.10 Da        Isoelectric Point: 5.4180

>NTDB_id=512662 I6G71_RS00150 WP_082308679.1 23609..25285(+) (pilF) [Neisseria meningitidis strain FDAARGOS_914]
MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGRLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=512662 I6G71_RS00150 WP_082308679.1 23609..25285(+) (pilF) [Neisseria meningitidis strain FDAARGOS_914]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGAGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAGACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATCCATTTCGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCGGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCCTACGGGATGGTGCTGGTAACCGGTCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGTCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGCTTCACCGATGAGGACCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(178-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

99.283

100

0.993
  pilB Acinetobacter baumannii D1279779

50.441

100

0.513
  pilB Acinetobacter baylyi ADP1

50.534

100

0.509
  pilB Legionella pneumophila strain ERS1305867

47.703

100

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

45.567

100

0.461
  pilB Vibrio cholerae strain A1552

45.552

100

0.459
  pilB Vibrio campbellii strain DS40M4

44.014

100

0.448
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.771

93.728

0.373
  pilF Thermus thermophilus HB27

36.949

97.491

0.36