Detailed information NTG0512663

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: I6G71_RS00165
• Genome accession: NZ_CP065653
• Coordinates: 26206..27066 (-)
• Length: 286 a.a.
• NCBI ID: WP_002226036.1
• Uniprot ID: A0A112F5U3
• Organism: Neisseria meningitidis strain FDAARGOS_914
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 21206..32066

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G71_RS00125 (I6G71_00125)	rplU	21210..21518 (-)	309	WP_002216394.1	50S ribosomal protein L21	-
I6G71_RS00135 (I6G71_00135)	ispB	21742..22716 (+)	975	WP_002216396.1	octaprenyl diphosphate synthase	-
I6G71_RS00140 (I6G71_00140)	-	22721..23167 (+)	447	WP_002233503.1	DUF441 domain-containing protein	-
I6G71_RS00150 (I6G71_00150)	pilF	23609..25285 (+)	1677	WP_082308679.1	type IV-A pilus assembly ATPase PilB	Machinery gene
I6G71_RS00155 (I6G71_00155)	-	25370..25579 (-)	210	WP_002249247.1	DNA gyrase inhibitor YacG	-
I6G71_RS00160 (I6G71_00160)	coaE	25572..26204 (-)	633	WP_002226983.1	dephospho-CoA kinase	-
I6G71_RS00165 (I6G71_00165)	pilD	26206..27066 (-)	861	WP_002226036.1	prepilin peptidase	Machinery gene
I6G71_RS00170 (I6G71_00170)	pilG	27140..28372 (-)	1233	WP_002248468.1	type 4 pilus assembly protein PilG	Machinery gene
I6G71_RS00175 (I6G71_00175)	pgi	28882..30525 (+)	1644	WP_002249249.1	glucose-6-phosphate isomerase	-
I6G71_RS00180 (I6G71_00180)	-	30495..30725 (+)	231	WP_002212338.1	hypothetical protein	-
I6G71_RS00185 (I6G71_00185)	dapD	30735..31556 (+)	822	WP_002226039.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31389.43 Da
• Isoelectric Point: 8.2213

>NTDB_id=512663 I6G71_RS00165 WP_002226036.1 26206..27066(-) (pilD) [Neisseria meningitidis strain FDAARGOS_914]
MSDLSVLSPFAVPLTAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide

• Length: 861 bp

>NTDB_id=512663 I6G71_RS00165 WP_002226036.1 26206..27066(-) (pilD) [Neisseria meningitidis strain FDAARGOS_914]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGACAGCGGTGTTCGGGCTGCTGGTCGGAAGTTTCTTAAA
TGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAGAATATTTAAACCTGCCGCTGA
CCGAAGAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCCTCCTGCCAAACCAAAATCAGCATACGCTATCC
CTTAATCGAACTGCTGACCGGCGTATTGTTCGGGCTGGTTGCCTGGCAATACGGCTGGTCTTGGATTACATTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACATTACCC
TTGATTTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCGATATGGCTTTTATGCGCGGTATATAAACTGATTAAAGGCGAAATCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACAGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (134-242)

3D structure

• AlphaFold DB: A0A112F5U3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	95.789	99.65	0.955
pilD	Vibrio cholerae strain A1552	44.097	100	0.444
pilD	Acinetobacter baumannii D1279779	46.97	92.308	0.434
pilD	Acinetobacter nosocomialis M2	46.97	92.308	0.434
pilD	Vibrio campbellii strain DS40M4	44.565	96.503	0.43