Detailed information NTG0511775

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: I5367_RS12105
• Genome accession: NZ_CP065539
• Coordinates: 2506784..2507398 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus amyloliquefaciens strain DGL1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2501784..2512398

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I5367_RS12075 (I5367_12075)	rpsT	2502087..2502353 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
I5367_RS12080 (I5367_12080)	holA	2502370..2503411 (-)	1042	Protein_2346	DNA polymerase III subunit delta	-
I5367_RS12085 (I5367_12085)	-	2503451..2503602 (-)	152	Protein_2347	hypothetical protein	-
I5367_RS12090 (I5367_12090)	-	2503643..2503777 (+)	135	WP_003152870.1	YqzM family protein	-
I5367_RS12095 (I5367_12095)	comEC	2503796..2506147 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
I5367_RS12100 (I5367_12100)	-	2506148..2506717 (-)	570	WP_003152868.1	ComE operon protein 2	-
I5367_RS12105 (I5367_12105)	comEA	2506784..2507398 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
I5367_RS12110 (I5367_12110)	comER	2507457..2508278 (+)	822	WP_012118027.1	late competence protein ComER	-
I5367_RS12115 (I5367_12115)	-	2508347..2509084 (-)	738	WP_012118028.1	class I SAM-dependent DNA methyltransferase	-
I5367_RS12120 (I5367_12120)	rsfS	2509081..2509437 (-)	357	WP_007408260.1	ribosome silencing factor	-
I5367_RS12125 (I5367_12125)	yqeK	2509454..2510014 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
I5367_RS12130 (I5367_12130)	-	2510004..2510573 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
I5367_RS12135 (I5367_12135)	yhbY	2510584..2510874 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
I5367_RS12140 (I5367_12140)	aroE	2510868..2511710 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=511775 I5367_RS12105 WP_012118026.1 2506784..2507398(-) (comEA) [Bacillus amyloliquefaciens strain DGL1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=511775 I5367_RS12105 WP_012118026.1 2506784..2507398(-) (comEA) [Bacillus amyloliquefaciens strain DGL1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363