Detailed information NTG0051108

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: SPAF_RS01620
• Genome accession: NC_017905
• Coordinates: 305992..306444 (+)
• Length: 150 a.a.
• NCBI ID: WP_003019060.1
• Uniprot ID: I1ZJT7
• Organism: Streptococcus parasanguinis FW213
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 300992..311444

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPAF_RS01585 (Spaf_0282)	-	301329..302432 (+)	1104	WP_014712775.1	glycosyl hydrolase family 8	-
SPAF_RS01590 (Spaf_0283)	-	302658..303050 (+)	393	WP_041826517.1	DUF1033 family protein	-
SPAF_RS01595 (Spaf_0284)	comYA	303136..304077 (+)	942	WP_003019098.1	competence type IV pilus ATPase ComGA	Machinery gene
SPAF_RS01600 (Spaf_0286)	comYB	304010..305041 (+)	1032	WP_115276785.1	competence type IV pilus assembly protein ComGB	Machinery gene
SPAF_RS01605 (Spaf_0287)	comYC	305038..305355 (+)	318	WP_003019171.1	competence type IV pilus major pilin ComGC	Machinery gene
SPAF_RS01610 (Spaf_0288)	comYD	305345..305749 (+)	405	WP_003004513.1	competence type IV pilus minor pilin ComGD	Machinery gene
SPAF_RS01615 (Spaf_0289)	comGE	305715..306002 (+)	288	WP_041826198.1	competence type IV pilus minor pilin ComGE	-
SPAF_RS01620 (Spaf_0290)	comGF/cglF	305992..306444 (+)	453	WP_003019060.1	competence type IV pilus minor pilin ComGF	Machinery gene
SPAF_RS01625 (Spaf_0291)	comGG	306401..306868 (+)	468	WP_014712781.1	competence type IV pilus minor pilin ComGG	-
SPAF_RS01630 (Spaf_0292)	comYH	306899..307852 (+)	954	WP_014712782.1	class I SAM-dependent methyltransferase	Machinery gene
SPAF_RS01635 (Spaf_0293)	-	307904..309097 (+)	1194	WP_013904491.1	acetate kinase	-
SPAF_RS01640 (Spaf_0294)	-	309170..309901 (+)	732	WP_014712783.1	type II CAAX endopeptidase family protein	-
SPAF_RS01645 (Spaf_0295)	folP	310063..311013 (+)	951	WP_003019077.1	dihydropteroate synthase	-

Sequence

Protein

• Length: 150 a.a.
• Molecular weight: 17355.77 Da
• Isoelectric Point: 5.5711

>NTDB_id=51108 SPAF_RS01620 WP_003019060.1 305992..306444(+) (comGF/cglF) [Streptococcus parasanguinis FW213]
MWNKEKVKAFTLLEALVALLVLSGGVLVFQGLTKLLHAELDYQAHQKQEEWILFQQQLQVELDRSHFEKVADNHIYLVQD
QKPIAIGQSKGDDIRKTDDKGRGYQPMISGVRSSQIWQEGDLLHLRLDFEEGLEREYVYHVVPAIQNEKS

Nucleotide

• Length: 453 bp

>NTDB_id=51108 SPAF_RS01620 WP_003019060.1 305992..306444(+) (comGF/cglF) [Streptococcus parasanguinis FW213]
ATGTGGAATAAAGAAAAGGTCAAGGCCTTTACCCTTCTCGAAGCCTTGGTCGCTCTCTTAGTGCTTAGTGGGGGAGTGTT
GGTCTTTCAAGGCTTGACCAAGCTTCTGCATGCTGAATTGGACTACCAGGCGCATCAAAAGCAGGAAGAATGGATACTTT
TTCAGCAACAATTGCAGGTGGAATTGGACCGGAGTCATTTTGAAAAAGTAGCGGACAATCATATCTACTTGGTTCAGGAT
CAAAAACCAATTGCTATTGGCCAATCTAAAGGAGATGATATCCGAAAAACAGATGATAAGGGCAGGGGCTATCAGCCTAT
GATTTCAGGTGTTCGCTCTAGCCAAATTTGGCAGGAGGGAGATTTGCTTCATCTGCGGTTGGATTTTGAAGAAGGTCTAG
AAAGGGAGTATGTCTACCATGTGGTACCAGCGATACAAAATGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (43-134)

3D structure

• AlphaFold DB: I1ZJT7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus mitis NCTC 12261	55.396	92.667	0.513
comGF/cglF	Streptococcus mitis SK321	54.676	92.667	0.507
comGF/cglF	Streptococcus pneumoniae Rx1	55.882	90.667	0.507
comGF/cglF	Streptococcus pneumoniae D39	55.882	90.667	0.507
comGF/cglF	Streptococcus pneumoniae R6	55.882	90.667	0.507
comGF/cglF	Streptococcus pneumoniae TIGR4	55.882	90.667	0.507
comYF	Streptococcus mutans UA159	42.647	90.667	0.387
comYF	Streptococcus mutans UA140	42.647	90.667	0.387
comGF	Lactococcus lactis subsp. cremoris KW2	39.007	94	0.367