NTDB

Overview

Name	comGF/cglF	Type	Machinery gene
Locus tag	I5V48_RS00765	Genome accession	NZ_CP065430
Coordinates	130737..131171 (+)	Length	144 a.a.
NCBI ID	WP_043026797.1	Uniprot ID	A0A0Z8IB52
Organism	Streptococcus suis strain YZDH1
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 125737..136171

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I5V48_RS00735 (I5V48_00735)	-	126879..127829 (-)	951	WP_043026809.1	S66 family peptidase	-
I5V48_RS00740 (I5V48_00740)	comYA	127915..128865 (+)	951	WP_043026808.1	competence type IV pilus ATPase ComGA	Machinery gene
I5V48_RS00745 (I5V48_00745)	comYB	128777..129814 (+)	1038	WP_043026806.1	competence type IV pilus assembly protein ComGB	Machinery gene
I5V48_RS00750 (I5V48_00750)	comYC	129816..130097 (+)	282	WP_043026803.1	competence type IV pilus major pilin ComGC	Machinery gene
I5V48_RS00755 (I5V48_00755)	comGD	130078..130485 (+)	408	WP_043026801.1	competence type IV pilus minor pilin ComGD	-
I5V48_RS00760 (I5V48_00760)	comYE	130457..130750 (+)	294	WP_043026799.1	competence type IV pilus minor pilin ComGE	Machinery gene
I5V48_RS00765 (I5V48_00765)	comGF/cglF	130737..131171 (+)	435	WP_043026797.1	competence type IV pilus minor pilin ComGF	Machinery gene
I5V48_RS00770 (I5V48_00770)	comGG	131149..131676 (+)	528	WP_043026795.1	competence type IV pilus minor pilin ComGG	-
I5V48_RS00775 (I5V48_00775)	comYH	131733..132686 (+)	954	WP_043026794.1	class I SAM-dependent methyltransferase	Machinery gene
I5V48_RS00780 (I5V48_00780)	-	132737..133924 (+)	1188	WP_043026792.1	acetate kinase	-
I5V48_RS00785 (I5V48_00785)	-	134239..134790 (+)	552	WP_043026790.1	folate family ECF transporter S component	-
I5V48_RS00790 (I5V48_00790)	-	134846..136102 (+)	1257	WP_043026788.1	bifunctional folylpolyglutamate synthase/dihydrofolate synthase	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 16462.85 Da Isoelectric Point: 8.8126

                                >NTDB_id=509502 I5V48_RS00765 WP_043026797.1 130737..131171(+) (comGF/cglF) [Streptococcus suis strain YZDH1]

MLKSKLPAFTLLECLVALIILSGSLLVFESLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGE

QALAFGKSRSDDFRKTNDRGQGYQPMIYQVDSAAISQKNQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide

Download Length: 435 bp

                                >NTDB_id=509502 I5V48_RS00765 WP_043026797.1 130737..131171(+) (comGF/cglF) [Streptococcus suis strain YZDH1]

TTGTTAAAAAGTAAACTTCCTGCTTTTACTCTCTTGGAATGCTTGGTAGCTTTAATCATCCTATCAGGAAGTCTTCTGGT

CTTTGAAAGCTTGTCAAAACTGCTGGTCCAAGAAGCGCATTACCAAGGTCAGACTGTTCAAAAGGAATGGTTGGTCTTCT

CTAGTCAATTGCGGTCGGAGTGGGACCAGGCTGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGGCGAA

CAGGCTTTGGCCTTTGGCAAGTCACGGTCGGATGATTTTCGGAAAACAAATGACAGGGGACAGGGCTATCAGCCCATGAT

TTATCAAGTAGACAGCGCAGCTATTTCCCAAAAAAACCAGCTGGTGCGCATCGACTTTAGCTTTAAAAACGGAGAGGAGC

GGACGTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(37-134)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0Z8IB52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF/cglF	Streptococcus pneumoniae D39	53.901	97.917	0.528
comGF/cglF	Streptococcus pneumoniae R6	53.901	97.917	0.528
comGF/cglF	Streptococcus pneumoniae TIGR4	53.901	97.917	0.528
comGF/cglF	Streptococcus pneumoniae Rx1	53.901	97.917	0.528
comYF	Streptococcus mutans UA140	49.306	100	0.493
comGF/cglF	Streptococcus mitis NCTC 12261	52.985	93.056	0.493
comYF	Streptococcus mutans UA159	48.611	100	0.486
comGF/cglF	Streptococcus mitis SK321	51.493	93.056	0.479
comGF	Lactococcus lactis subsp. cremoris KW2	43.972	97.917	0.431

Detailed information NTG0509502