Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I3K84_RS09055 Genome accession   NZ_CP065406
Coordinates   1895489..1897222 (+) Length   577 a.a.
NCBI ID   WP_012655751.1    Uniprot ID   A0A6M6IAK8
Organism   Diaphorobacter sp. JS3051     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1890489..1902222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3K84_RS09030 (I3K84_09030) - 1891137..1891445 (+) 309 WP_196995942.1 hypothetical protein -
  I3K84_RS09035 (I3K84_09035) - 1891473..1892981 (-) 1509 WP_196996092.1 IS66 family transposase -
  I3K84_RS09040 (I3K84_09040) tnpB 1893064..1893396 (-) 333 WP_044397668.1 IS66 family insertion sequence element accessory protein TnpB -
  I3K84_RS09045 (I3K84_09045) - 1893393..1893794 (-) 402 WP_044397671.1 transposase -
  I3K84_RS09050 (I3K84_09050) - 1894037..1895128 (-) 1092 WP_196995943.1 site-specific integrase -
  I3K84_RS09055 (I3K84_09055) pilB 1895489..1897222 (+) 1734 WP_012655751.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I3K84_RS09060 (I3K84_09060) pilC 1897242..1898459 (+) 1218 WP_088887536.1 type II secretion system F family protein Machinery gene
  I3K84_RS09065 (I3K84_09065) pilD 1898459..1899340 (+) 882 WP_196995944.1 A24 family peptidase Machinery gene
  I3K84_RS09070 (I3K84_09070) coaE 1899372..1899965 (+) 594 WP_231403448.1 dephospho-CoA kinase -
  I3K84_RS09075 (I3K84_09075) zapD 1900056..1900811 (+) 756 WP_011804250.1 cell division protein ZapD -
  I3K84_RS09080 (I3K84_09080) - 1900818..1901048 (+) 231 WP_011804249.1 DNA gyrase inhibitor YacG -
  I3K84_RS09085 (I3K84_09085) - 1901117..1901563 (-) 447 WP_011804248.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63629.02 Da        Isoelectric Point: 5.3559

>NTDB_id=509206 I3K84_RS09055 WP_012655751.1 1895489..1897222(+) (pilB) [Diaphorobacter sp. JS3051]
MAAIDTQKEAQPIALPGLARALMSVGKLDQKAAEEIYKKSQNSRSSFIAELTESKIITPSDLAHTVSTVFGAPLLDVDAI
DRQRIPKDLLDVKLCQSYKVIALSKRNNRLIVATADPTFQEAAEKIKFTTQMGVDWIIAEYDKLLRLIEASTKSGTETLD
GINSSNDFEFGDIPVDEAPDEDADNPAEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHHYRVRFRIDGELREIASPPI
AIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
LLQAINRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPNCKAPVDIPH
ETLLEAGYRDEEIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPVSEEIQRIILRDGSALEIAEQARLEGVRSLRESGLHK
AKMGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=509206 I3K84_RS09055 WP_012655751.1 1895489..1897222(+) (pilB) [Diaphorobacter sp. JS3051]
ATGGCTGCTATCGACACACAGAAAGAAGCTCAACCCATTGCCTTACCCGGACTGGCGCGCGCGTTGATGTCTGTGGGCAA
ATTGGATCAAAAAGCGGCGGAAGAGATTTACAAAAAGTCACAAAATAGCCGCAGTAGCTTTATTGCAGAGCTCACGGAAT
CGAAAATCATCACGCCAAGTGATCTAGCCCATACGGTATCCACCGTATTTGGTGCCCCCTTGCTAGATGTCGATGCGATC
GACCGGCAGCGCATCCCCAAAGATCTGTTGGACGTCAAACTGTGCCAGTCCTACAAAGTCATCGCTCTCAGTAAGCGCAA
TAACCGGCTCATAGTCGCCACCGCGGATCCGACATTTCAAGAGGCCGCAGAAAAGATCAAGTTCACCACACAAATGGGGG
TGGACTGGATCATCGCGGAATATGACAAACTGCTCCGCTTGATTGAGGCGAGCACAAAGAGCGGTACAGAAACGCTCGAC
GGCATTAACAGTAGTAATGATTTTGAGTTCGGCGACATTCCCGTTGATGAAGCTCCGGACGAGGATGCCGACAATCCCGC
GGAAGTCGAGGATGCTCCCGTTGTCAAATTTCTGCACAAGATGCTGCTGGATGCCTTCAATATGCGTGCATCCGATCTGC
ATTTTGAGCCCTACGAGCACCATTACCGTGTCCGCTTCCGCATAGATGGCGAATTGCGCGAAATCGCATCGCCGCCAATT
GCCATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCCCGCCTGGATATTTCGGAAAAACGCGTGCCCCAGGATGG
CCGCATGAAGCTCAAGGTTGGGCCGGACCGGGTTATCGATTTCCGCGTCAGCACCCTGCCTACGCTCTTTGGCGAGAAGA
TCGTGATCCGTATTCTCGATCCGAGCAGCGCCAAGCTGGGTATTGACGCACTGGGATATGAGCCCGAGGAAAAGGAGCGT
TTGCTGCAGGCCATCAACCGCCCTTATGGAATGATCCTGGTGACCGGACCCACGGGCTCCGGGAAGACCGTGTCGCTCTA
CACATGTCTCAATTTGTTGAACAAGCCCGGCGTCAATATTGCGACCGCTGAGGATCCCTCGGAAATCAACCTCCCGGGTG
TGAACCAAGTCAACGTCAACGAAAAAGCCGGCCTCACGTTTGCCGTCGCGCTCAAGTCATTCCTTCGCCAAGATCCGGAC
ATCATCATGGTGGGTGAAATCCGGGATCTGGAAACGGCAGACATTTCGATCAAAGCCGCGCAGACGGGTCACTTGGTGCT
GTCCACCCTGCACACCAATGACGCCCCCACGACATTGACGCGCATGCGCAATATGGGTATCGCGCCCTTCAATATCGCAT
CCAGCGTGATTCTTATTACGGCGCAGCGACTGGCGCGTCGCTTGTGCCCCAATTGCAAGGCGCCCGTGGACATTCCACAT
GAGACGCTGCTGGAGGCCGGCTACCGGGACGAGGAGATCGACGGCTCATGGGTCACCTACCGACCGGTGGGCTGCTCCGC
CTGCAATAACGGATACAAGGGGCGCGTGGGCATTTACCAGGTCATGCCGGTGTCCGAAGAAATCCAGCGCATCATCCTGC
GCGATGGAAGCGCACTGGAAATCGCCGAACAGGCACGCCTGGAAGGCGTCCGTTCTCTGCGTGAATCCGGGCTTCACAAG
GCAAAAATGGGATTGACCTCCTTGGAAGAGGTCTTGGCTGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M6IAK8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.181

99.48

0.539
  pilB Acinetobacter baumannii D1279779

53.723

97.747

0.525
  pilF Neisseria gonorrhoeae MS11

52.389

97.92

0.513
  pilB Legionella pneumophila strain ERS1305867

49.912

97.92

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

45.714

97.054

0.444
  pilB Vibrio campbellii strain DS40M4

45.455

97.227

0.442
  pilB Vibrio cholerae strain A1552

46.881

91.681

0.43
  pilF Thermus thermophilus HB27

39.897

100

0.404
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.121

92.721

0.381