Detailed information NTG0507519

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: I3J23_RS02730
• Genome accession: NZ_CP065159
• Coordinates: 517060..517674 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain GXU-1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 512060..522674

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I3J23_RS02700 (I3J23_02700)	rpsT	512360..512626 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
I3J23_RS02705 (I3J23_02705)	holA	512643..513684 (-)	1042	Protein_541	DNA polymerase III subunit delta	-
I3J23_RS02710 (I3J23_02710)	-	513724..513878 (-)	155	Protein_542	hypothetical protein	-
I3J23_RS02715 (I3J23_02715)	-	513919..514053 (+)	135	WP_003152870.1	YqzM family protein	-
I3J23_RS02720 (I3J23_02720)	comEC	514072..516423 (-)	2352	WP_041481887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
I3J23_RS02725 (I3J23_02725)	-	516424..516993 (-)	570	WP_003152868.1	ComE operon protein 2	-
I3J23_RS02730 (I3J23_02730)	comEA	517060..517674 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
I3J23_RS02735 (I3J23_02735)	comER	517733..518554 (+)	822	WP_014305449.1	late competence protein ComER	-
I3J23_RS02740 (I3J23_02740)	-	518623..519360 (-)	738	WP_032856885.1	class I SAM-dependent methyltransferase	-
I3J23_RS02745 (I3J23_02745)	rsfS	519357..519713 (-)	357	WP_003152864.1	ribosome silencing factor	-
I3J23_RS02750 (I3J23_02750)	yqeK	519731..520291 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
I3J23_RS02755 (I3J23_02755)	-	520281..520850 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
I3J23_RS02760 (I3J23_02760)	yhbY	520861..521151 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
I3J23_RS02765 (I3J23_02765)	aroE	521145..521987 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=507519 I3J23_RS02730 WP_003152867.1 517060..517674(-) (comEA) [Bacillus amyloliquefaciens strain GXU-1]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=507519 I3J23_RS02730 WP_003152867.1 517060..517674(-) (comEA) [Bacillus amyloliquefaciens strain GXU-1]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363