Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IV454_RS14130 Genome accession   NZ_CP065053
Coordinates   3122147..3123874 (-) Length   575 a.a.
NCBI ID   WP_054266483.1    Uniprot ID   -
Organism   Massilia antarctica strain P8398     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3117147..3128874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IV454_RS14105 (IV454_14095) yacG 3118140..3118325 (-) 186 WP_126074530.1 DNA gyrase inhibitor YacG -
  IV454_RS14110 (IV454_14100) zapD 3118339..3119094 (-) 756 WP_054266479.1 cell division protein ZapD -
  IV454_RS14115 (IV454_14105) coaE 3119156..3119812 (-) 657 WP_206091951.1 dephospho-CoA kinase -
  IV454_RS14120 (IV454_14110) - 3119813..3120706 (-) 894 WP_206091952.1 A24 family peptidase -
  IV454_RS14125 (IV454_14115) pilC 3120909..3122135 (-) 1227 WP_206091953.1 type II secretion system F family protein Machinery gene
  IV454_RS14130 (IV454_14120) pilB 3122147..3123874 (-) 1728 WP_054266483.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IV454_RS14135 (IV454_14125) - 3124086..3125366 (-) 1281 WP_206091954.1 HlyC/CorC family transporter -
  IV454_RS14140 (IV454_14130) - 3125592..3126152 (-) 561 WP_054266485.1 ATP-dependent Clp protease proteolytic subunit -
  IV454_RS14145 (IV454_14135) uraH 3126220..3126573 (-) 354 WP_054266486.1 hydroxyisourate hydrolase -
  IV454_RS14150 (IV454_14140) puuE 3126696..3127628 (+) 933 WP_206091955.1 allantoinase PuuE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62512.32 Da        Isoelectric Point: 5.5798

>NTDB_id=506269 IV454_RS14130 WP_054266483.1 3122147..3123874(-) (pilB) [Massilia antarctica strain P8398]
MAAVLPNTTSGTPMPGLARALMQAGRLSAQQADTLHKKSLTDKSPFIDTLLLTGTFDARTLAAFCAETFGYPLMDLQSFN
IDSLPPNAIDGKLMQAQRVIALAKRGNKMSVAISDPTNIQALDQIKFQSEASVEPVIVLHDALLTLLASLGRNAEQNLNE
MVGDQEDIQFGEDDAPAVVDQAAAADIEDAPIVKFLNKILMDAINLGASDIHFEPFEKFYRIRLRVDGMLKEHAQPPITI
RDKLVSRIKVLSKLDISEKRVPQDGRMRLILSPTKAIDFRVSTCPTLFGEKTVMRILDATQAQMGIDALGYDPDQKELLL
EAIQRPYGMVLVTGPTGSGKTVSLYTCLNVINKPGINISTAEDPAEINLPGVNQVNINERSGLTFPVALKSFLRQDPDII
MVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVIMITAQRLARRLCTCKKPVETSREIL
LAAGFKEDELEGGWKPYGPVGCDRCLGGGYKGRVGIYQIMPITSAIEKIILAHGNSMEIAAQSELEGVKSLRRSGLMKVK
QGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=506269 IV454_RS14130 WP_054266483.1 3122147..3123874(-) (pilB) [Massilia antarctica strain P8398]
ATGGCAGCAGTCCTCCCCAACACGACCTCCGGAACGCCCATGCCGGGCCTGGCCCGCGCCTTGATGCAGGCTGGCCGCCT
CAGTGCGCAGCAAGCCGATACCTTGCACAAGAAATCGCTGACCGACAAGAGTCCGTTCATCGACACCTTGCTGCTGACCG
GCACCTTCGACGCGCGCACCCTGGCCGCCTTTTGCGCGGAAACCTTCGGCTACCCGCTGATGGATTTGCAGAGCTTTAAT
ATCGATTCCCTGCCGCCGAATGCCATCGACGGCAAGCTGATGCAGGCCCAGCGCGTGATCGCCCTGGCCAAGCGCGGCAA
CAAGATGTCGGTCGCCATTTCGGACCCCACCAATATCCAGGCGCTCGACCAGATCAAGTTCCAGAGCGAAGCGTCGGTCG
AGCCGGTGATCGTGCTGCACGACGCCCTGCTGACCCTGCTGGCCAGCTTGGGCCGCAACGCCGAACAGAATCTGAACGAG
ATGGTCGGCGACCAGGAAGATATCCAGTTCGGCGAAGACGATGCGCCCGCCGTGGTCGACCAGGCCGCCGCCGCCGACAT
CGAAGACGCGCCGATCGTCAAGTTCCTCAACAAGATCCTGATGGACGCGATCAACCTCGGCGCGTCCGATATCCACTTCG
AGCCCTTCGAAAAGTTTTACCGCATCCGCCTGCGCGTCGATGGCATGCTGAAAGAACATGCCCAGCCGCCGATCACCATC
CGCGACAAGCTGGTCTCGCGCATCAAGGTGCTCTCCAAGCTCGACATTTCCGAAAAGCGCGTGCCGCAGGATGGCCGCAT
GCGCCTGATCCTCTCGCCCACCAAGGCGATCGACTTCCGGGTCAGCACCTGCCCTACCCTGTTCGGCGAAAAGACCGTCA
TGCGGATCCTGGACGCCACCCAGGCCCAGATGGGCATCGACGCGCTCGGCTACGATCCGGACCAGAAGGAATTGCTGCTC
GAAGCGATCCAGCGTCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCCGGCAAGACGGTGTCGCTGTACACCTG
CCTGAACGTGATCAACAAGCCTGGCATCAACATTTCGACCGCGGAAGACCCGGCCGAGATCAACTTGCCGGGCGTGAACC
AGGTCAACATCAACGAACGCTCGGGCCTGACCTTCCCGGTCGCGCTCAAGTCCTTCCTGCGCCAGGATCCGGACATCATC
ATGGTCGGCGAGATCCGCGACCTTGAAACGGCCGACATCGCCATCAAGGCCGCCCAGACCGGCCACATGGTGTTCTCGAC
CCTGCACACCAACGATGCGCCCTCGACCCTGACCCGGTTGATGAACATGGGCGTGGCGCCGTTCAACATCGCCTCCTCGG
TCATCATGATCACGGCGCAGCGCCTGGCGCGGCGCTTGTGCACCTGCAAGAAGCCGGTGGAAACCAGCCGCGAAATCCTG
CTCGCGGCCGGCTTCAAGGAAGACGAACTGGAAGGCGGCTGGAAGCCTTACGGACCGGTCGGCTGCGACCGTTGCCTGGG
CGGCGGCTACAAGGGCCGGGTCGGCATTTACCAGATCATGCCGATTACCTCGGCGATCGAAAAAATCATTCTGGCGCACG
GCAATTCGATGGAAATTGCCGCGCAATCGGAGCTGGAAGGCGTCAAATCCCTGCGCCGTTCCGGCCTGATGAAAGTAAAA
CAAGGCTTGACCAGCCTTGAAGAAGTTCTTGGCTGCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-155)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.887

98.435

0.53
  pilB Acinetobacter baylyi ADP1

52.9

98.957

0.523
  pilF Neisseria gonorrhoeae MS11

52.931

97.913

0.518
  pilB Legionella pneumophila strain ERS1305867

48.932

97.739

0.478
  pilB Vibrio cholerae strain A1552

44.938

97.913

0.44
  pilB Vibrio parahaemolyticus RIMD 2210633

44.982

97.043

0.437
  pilB Vibrio campbellii strain DS40M4

44.346

98.435

0.437
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.163

98.435

0.376
  pilF Thermus thermophilus HB27

40

93.043

0.372